Difference between revisions of "Team:Tsinghua"
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| − | {{Tsinghua | + | {{Tsinghua}} |
| − | <html lang="en | + | <html lang="en-US"> |
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| − | <meta name="viewport" content="width=device-width, initial-scale=1"> | + | <meta name="viewport" content="width=device-width, initial-scale=1"> |
| − | < | + | <!-- --> |
| − | < | + | <!-- Document Title--> |
| − | < | + | <!-- =============================================--> |
| − | + | <title>iGEM Tsinghua</title> | |
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| − | + | <!-- <link rel="apple-touch-icon" sizes="180x180" href="assets/images/favicons/apple-touch-icon.png"> | |
| − | + | <link rel="icon" type="image/png" sizes="32x32" href="assets/images/favicons/favicon-32x32.png"> | |
| − | + | <link rel="icon" type="image/png" sizes="16x16" href="assets/images/favicons/favicon-16x16.png"> | |
| − | + | <link rel="shortcut icon" type="image/x-icon" href="assets/images/favicons/favicon.ico"> | |
| + | <link rel="manifest" href="assets/images/favicons/manifest.json"> | ||
| + | <link rel="mask-icon" href="assets/images/favicons/safari-pinned-tab.svg" color="#5bbad5"> | ||
| + | <meta name="msapplication-TileImage" content="assets/images/favicons/mstile-150x150.png"> | ||
| + | <meta name="theme-color" content="#ffffff"> | ||
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| − | <link href="assets/lib/bootstrap/dist/css/bootstrap.min.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/bootstrap/dist/css/bootstrap.min.css" rel="stylesheet"> --> |
| + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/bootstrap.min.css&action=raw&ctype=text/css" > | ||
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<!-- Template specific stylesheets--> | <!-- Template specific stylesheets--> | ||
| − | <link href="assets/lib/loaders.css/loaders.min.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/loaders.css/loaders.min.css" rel="stylesheet"> --> |
<link href="https://fonts.googleapis.com/css?family=Hind+Madurai:300,400,500,600,700|Playfair+Display:400,400i,700,700i,900,900i" rel="stylesheet"> | <link href="https://fonts.googleapis.com/css?family=Hind+Madurai:300,400,500,600,700|Playfair+Display:400,400i,700,700i,900,900i" rel="stylesheet"> | ||
| − | <link href="assets/lib/hamburgers/dist/hamburgers.min.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/hamburgers/dist/hamburgers.min.css" rel="stylesheet"> --> |
| − | <link href="assets/lib/font-awesome/css/font-awesome.min.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/font-awesome/css/font-awesome.min.css" rel="stylesheet"> --> |
| − | <link href="assets/lib/lightbox2/dist/css/lightbox.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/lightbox2/dist/css/lightbox.css" rel="stylesheet"> --> |
| − | <link href="assets/lib/flexslider/flexslider.css" rel="stylesheet"> | + | <!-- <link href="assets/lib/flexslider/flexslider.css" rel="stylesheet"> --> |
| + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/flexslider2.css&action=raw&ctype=text/css" > | ||
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| − | <link href="assets/css/style.css" rel="stylesheet"> | + | <!-- <link href="assets/css/style.css" rel="stylesheet"> --> |
| − | <link href="assets/css/custom.css" rel="stylesheet"> | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/font-awesome.min.css&action=raw&ctype=text/css"> |
| − | </head> | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/style.css&action=raw&ctype=text/css" > |
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| − | <body data-spy="scroll" data-target=".inner-link" data-offset="60"> | + | <!-- <link href="assets/css/custom.css" rel="stylesheet"> </head> --> |
| − | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/custom.css&action=raw&ctype=text/css" > | |
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| − | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/remodal.css&action=raw&ctype=text/css" > | |
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| − | + | <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Team:Tsinghua/css/remodal-default-theme.css&action=raw&ctype=text/css" > | |
| − | + | <style> | |
| − | + | *{margin:0;padding:0;} | |
| + | .box{height:1px;width:1%;background:#ccc;} | ||
| + | .progress{position:fixed;top:1rem; z-index:9999;height:5px;background-image: linear-gradient(to right, rgb(252, 141, 15),rgb(14, 178, 243));} | ||
| + | </style> | ||
| + | </head> | ||
| + | <body data-spy="scroll" data-target=".inner-link" data-offset="60"> | ||
| + | <main> | ||
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| + | <div class="loading" id="preloader"> | ||
| + | <div class="h-100vh d-flex align-items-center justify-content-center background-white"> | ||
| + | <div> | ||
| + | <img src="https://static.igem.org/mediawiki/2019/1/15/T--Tsinghua--loading.gif" style="width:5%; position:absolute; left: 46%; top: 43%"> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="znav-container znav-transparent | + | </div> |
| + | <div class="sticky-lg-top pt-4" style="z-index: 1000;"> | ||
| + | <div style="background: rgba(30, 30, 30, 0.936);position: absolute; width: 100%; height: 10rem;top:-3.2rem;"></div> | ||
| + | <div class="znav-container znav-transparent sticky-lg-top pt-4 mt-0" id="znav-container" style="left:10%"> | ||
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<div class="container"> | <div class="container"> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2019/b/be/T--Tsinghua--logo.png" style="width: 8%;position: absolute;left: -6%;"> | |
<nav class="navbar navbar-expand-lg"> | <nav class="navbar navbar-expand-lg"> | ||
| − | <a class="navbar-brand overflow-hidden pr-3 fs-3" href=" | + | |
| + | <a class="navbar-brand overflow-hidden pr-3 fs-3" href="https://2019.igem.org/Team:Tsinghua"> | ||
| + | |||
| + | iGEM | ||
<span class="d-none d-lg-inline-block">Tsinghua</span> | <span class="d-none d-lg-inline-block">Tsinghua</span> | ||
</a> | </a> | ||
| − | <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarNavDropdown" aria-controls="navbarNavDropdown" aria-expanded="false" aria-label="Toggle navigation"> | + | <!-- <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarNavDropdown" aria-controls="navbarNavDropdown" aria-expanded="false" aria-label="Toggle navigation"> |
<div class="hamburger hamburger--emphatic"> | <div class="hamburger hamburger--emphatic"> | ||
<div class="hamburger-box"> | <div class="hamburger-box"> | ||
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</div> | </div> | ||
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| − | </button> | + | </button> --> |
<div class="collapse navbar-collapse" id="navbarNavDropdown"> | <div class="collapse navbar-collapse" id="navbarNavDropdown"> | ||
<ul class="navbar-nav font-2 fs-1"> | <ul class="navbar-nav font-2 fs-1"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua"><span class="fa fa-home"></span> Home</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | + | <a href="JavaScript:void(0)"><span class="fa fa-group"></span> Team</a> | |
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| − | <a href="JavaScript:void(0)"> | + | |
<ul class="dropdown fs-0"> | <ul class="dropdown fs-0"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Team">Members</a> |
| − | </li> | + | </li> |
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Collaborations">Collaborations</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Notebook">Notebook</a> |
</li> | </li> | ||
| + | |||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Attributions">Attributions</a> |
</li> | </li> | ||
| − | |||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Design">Design</a> |
</li> | </li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="JavaScript:void(0)"> | + | <a href="JavaScript:void(0)"><span class="fa fa-flask"></span> Project</a> |
<ul class="dropdown fs-0"> | <ul class="dropdown fs-0"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Description">Description</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Experiments">Experiments</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Demonstrate">Results</a> |
</li> | </li> | ||
| + | <li> | ||
| + | <a href="https://2019.igem.org/Team:Tsinghua/Measurement">Measurement</a> | ||
| + | </li> | ||
| + | |||
</ul> | </ul> | ||
</li> | </li> | ||
<li> | <li> | ||
| − | <a href="JavaScript:void(0)"> | + | <a href="JavaScript:void(0)"><span class="fa fa-cube"></span> Model</a> |
<ul class="dropdown fs-0"> | <ul class="dropdown fs-0"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Model">Part I</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Model2">Part II</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Model3">Part III</a> |
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</li> | </li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | |||
<li> | <li> | ||
| − | <a href="JavaScript:void(0)"> | + | <a href="JavaScript:void(0)"><span class="fa fa-gears"></span> Parts</a> |
<ul class="dropdown fs-0"> | <ul class="dropdown fs-0"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Improvement">Improvement</a> |
</li> | </li> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Characterization">Characterization</a> |
</li> | </li> | ||
| + | |||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Chart">Chart Form</a> |
</li> | </li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | |||
| + | <li> | ||
| + | <a href="JavaScript:void(0)"><span class="fa fa-male"></span> Human Practices</a> | ||
| + | <ul class="dropdown fs-0"> | ||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Human_Practices">General</a> |
</li> | </li> | ||
| + | |||
<li> | <li> | ||
| − | <a href=" | + | <a href="https://2019.igem.org/Team:Tsinghua/Integrated">Integrated</a> |
</li> | </li> | ||
| + | <li> | ||
| + | <a href="https://2019.igem.org/Team:Tsinghua/Safety">Safety</a> | ||
| + | </li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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| + | <img src="https://static.igem.org/mediawiki/2019/1/11/T--Tsinghua--whitemask.png"></a> | ||
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| + | </div> | ||
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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</div> | </div> | ||
| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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</div> | </div> | ||
| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
| − | + | ||
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</div> | </div> | ||
| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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</div> | </div> | ||
| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| − | <!--/. | + | <!--/.row--> |
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| − | + | <div class="background-holder overlay" style="background-image:url(https://static.igem.org/mediawiki/2019/3/34/T--Tsinghua--indexSlider6.jpg);"> </div> | |
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| − | + | <a class="down-indicator" href="#target-down-indicator"></a> | |
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| − | + | <div class="overflow-hidden"> | |
| − | + | <h4 class="color-white fs-4 fs-lg-7" data-zanim='{"delay":0.1}'>PhASE | Photo-Activated Switch in <i>E.coli</i></h4> | |
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</div> | </div> | ||
| + | <div class="overflow-hidden"> | ||
| + | <h6 class="text-uppercase color-white fs-1 fs-lg-0 font-2 ls" data-zanim='{"delay":0.2}'>Tsinghua 2019</h6> | ||
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| + | <div class="index_para" style="width: 90%;"><h1>Phase separation system</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm; text-align: justify;">We constructed the first artificial phase separation system ever in prokaryotes. These droplets in E.coli exhibit similar spherical shape and high fluidity as they were in eukaryotes. By creating membraneless organelles in a cell, phases could realize the redistribution of various targets, including proteins and small organic molecules.</p> | ||
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| + | <div class="index_para" style="width: 100%;"><h1>Photo-activated switch</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">We use laser as a switch to control our system in a high spatial-temporal resolution. Within a few seconds, target proteins would redistribute into existing phases. After incubated in dark for several minutes, targets can reverse to previous smear state.</p> | ||
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| − | + | <div class="index_para" style="width: 85%;"><h1>Modelling our system</h1> | |
| − | + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">We modelled the phase separation system and coupled enzymatic reactions using a differential equation model and a collision model of chemical reactions. The model conceptually explained how phase separation would regulate downstream reactions, and provided insights for enzyme concentration and phase separation system selection. In addition, an interactive interface based on the model is presented on our wiki.</p> | |
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| − | + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Enzymes of proper size could be recruited into phases by using photo-activated switch. Selecting substrates of proper solubility according to our model, we realized the regulation of enzymatic reactions.</p> | |
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| + | <div class="index_para" style="width: 84%;"><h1>Improvement</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Enrichment of CRY2 in phase include both homo-aggregation of itself and hetero-aggregation of CRY2 and CIB1. By introducing point mutation to C terminal of CRY2, we attenuate homo-aggregation of CRY2 in dark, increasing the threshold of light induction. This improvement may also be helpful for teams who want to modify the sensitivity of their light-controlled information-processing system.</p> | ||
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| + | <div class="index_para" style="width: 90%;"><h1>More possibilities</h1> | ||
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Upon introducing this powerful tool in E.coli, there are much more prosperous applications waiting to be developed. We propose that by isolating specific material in phase, a uniform E.coli colony could grow into a society with cell differentiation.</p> | ||
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| − | + | <div class="index_para" style="width: 84%;"><h1>Hardware</h1> | |
| + | <p class="fs-3" style="margin: 1cm 0 1cm 0; text-indent: 1cm;text-align: justify;">Depending on the manner of reversible amplification in phase, specific proteins can be used to create a biological register. Responding to laser stimulation, the distribution of fluorescence proteins would change, resulting in the variation in FRET signal, which can be detected by fluorescence reader.</p> | ||
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Latest revision as of 01:51, 22 October 2019
Phase separation system
We constructed the first artificial phase separation system ever in prokaryotes. These droplets in E.coli exhibit similar spherical shape and high fluidity as they were in eukaryotes. By creating membraneless organelles in a cell, phases could realize the redistribution of various targets, including proteins and small organic molecules.
Photo-activated switch
We use laser as a switch to control our system in a high spatial-temporal resolution. Within a few seconds, target proteins would redistribute into existing phases. After incubated in dark for several minutes, targets can reverse to previous smear state.
Modelling our system
We modelled the phase separation system and coupled enzymatic reactions using a differential equation model and a collision model of chemical reactions. The model conceptually explained how phase separation would regulate downstream reactions, and provided insights for enzyme concentration and phase separation system selection. In addition, an interactive interface based on the model is presented on our wiki.
Downstream reactions
Enzymes of proper size could be recruited into phases by using photo-activated switch. Selecting substrates of proper solubility according to our model, we realized the regulation of enzymatic reactions.
Improvement
Enrichment of CRY2 in phase include both homo-aggregation of itself and hetero-aggregation of CRY2 and CIB1. By introducing point mutation to C terminal of CRY2, we attenuate homo-aggregation of CRY2 in dark, increasing the threshold of light induction. This improvement may also be helpful for teams who want to modify the sensitivity of their light-controlled information-processing system.
More possibilities
Upon introducing this powerful tool in E.coli, there are much more prosperous applications waiting to be developed. We propose that by isolating specific material in phase, a uniform E.coli colony could grow into a society with cell differentiation.
Hardware
Depending on the manner of reversible amplification in phase, specific proteins can be used to create a biological register. Responding to laser stimulation, the distribution of fluorescence proteins would change, resulting in the variation in FRET signal, which can be detected by fluorescence reader.
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