1. Beijing regional meetup
On July 6th, we gladly accepted the invitation from BNU-China and took part in the Beijing regional meetup hosted by BNU-China in Beijing. BNU-China, Jiangnan_China, JNU_China and Peking participated in the meeting. We gave a presentation about our project. During discussion, we shared our opinion of other teams’ projects and also got practical advice from them.
Fig.1: BRM Meeting
2. the International Genetically Engineered Machine Competition Symposium in Beijing
On August 6th, we attended the International Genetically Engineered Machine Competition Symposium in Beijing hosted by BIT_China. In total eight teams attended the symposium and each team gave presentation about their projects. We benefitted a lot from the discussion during the symposium.
Fig.2: BIT Meeting
3. the Conference of China iGEMer Community (CCiC)
We took part in the Conference of China iGEMer Community (CCiC) from August 20th to August 23rd 2019 in Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences. Three teammates of Peking 2019 attended the meeting and our advisor Cheng Li volunteered as a judge. There are 70 teams in total that joined in CCiC 2019.
Fig.3: CCiC Family
Fig.4: CCiC Modeling
Our teammates gave a presentation on August 21st and we were asked several insightful questions which shed light on our experiments and reminded us of some potential new applications. Through the four-day communication with judges and other teams, we got a lot of excellent suggestions about our project, and also shared our opinion, experience and ideas with other teams. During CCiC, we also hosted a modelling session and had a discussion about modelling with modelers from other teams.
Collaborations with other teams
We shared the quorum sensing part, which had been well characterized and proved to be reliable by us, with BIT-China. We are pleased to share our experience of using it.
We shared our previous part BBa_K2601007 with SCU_China . Furthermore, during the 6th CCiC, we talked with SCU_China about our plan to test the metabolic capacity, and they generously offered a plasmid with FMO (an essential enzyme related to indigo production) , which made it possible to test the metabolic capacity of our system.
Fig.5: The indigo blue productivity experiment did with the FMO sequence provided from SCU-China
During the meeting in BNU in July 6th, we came to know that BNU-China was in need of integrase-related parts. By coincidence, we developed these parts in 2017 and had experience about using it. We gladly shared our previous part BBa_K2243006, BBa_K2243007, BBa_K2243010, BBa_K2243011 with BNU-China.
We shared our previous part BBa_K1689009 and BBa_K1689010 with SJTU_BioX_Shanghai. We are glad that our previous part contributed to the report system of their project.
Our project is promising serving as a safety control system in microbiome therapy, which is also focused on by UCAS-China. We are glad that UCAS-China generously provided us with temperature-control switch they developed and a kind of E. coli strain Nissle, which is used widely in microbiome therapy. We also had a discussion about human practice about microbiome therapy.
Tsinghua iGEM 2019 is developing a light-induce phase separation system in E. coli. In 2018, Peking developed a synthetic phase separation in S. cerevisae. We shared basic parts SUMO (BBa_K2601002) and SIM (BBa_K2601003) with Tsinghua and made several discussions in June and July about characterization and simulation of phase separation system. We are pleased our experience is helpful to them. During CCiC, Peking and Tsinghua had some discussion and found that we were both interested in observing cell-division. We shared our knowledge and insights on cell division with each other and used our microfluidic system to observe asymmetric division of cells which Tsinghua took interested in.
Peking is glad to share our experience with new teams for iGEM. This year we helped a new team CAU_China with team management and part improvement issues. During the meeting on August 6th, we shared our opinion about their project and gave somesuggestions on pathway construction, which was helpful for upgrade of their project.
Mentoring high-school teams: QHFZ-China and BNDS_China
Peking is enthusiastic about mentoring high-school teams. This year We instructed BNDS_China and QHFZ-China in experiment. We shared some reliable reagents with BNDS_China and helped them in system characterization and trouble-shooting. We helped QHFZ-China in molecular cloning, measuring and modelling. We also shared our experience about wiki construction. Thankfully, QHFZ-China gave use some practical and insightful advice to beautify our logo.
Fig.6: Collaboration with QHFZ-China