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{{Humboldt_Berlin}} | {{Humboldt_Berlin}} | ||
<html> | <html> | ||
+ | |||
<div class="has-animations"> | <div class="has-animations"> | ||
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<div class="fixed-header-container"> | <div class="fixed-header-container"> | ||
− | + | <section class="fixed-image-header"> | |
− | + | <img src="https://static.igem.org/mediawiki/2019/8/81/T--Humboldt_Berlin--Notebook_Header.jpeg" alt="notebook" /> | |
− | + | </section> | |
− | + | <h1 class="page-headline description">Notebook</h1> | |
− | + | </div> | |
− | + | <div class="full-height fixed-header-content notebook"> | |
− | + | <div class="width-limit padding-container" style="margin: 50px 0;"> | |
− | + | <img src="https://static.igem.org/mediawiki/2019/e/e3/T--Humboldt_Berlin--notebook_final.png" alt="our stuffed calendar"/></br> | |
− | + | </div> | |
+ | <section class="width-limit"> | ||
+ | <div class="category-container"> | ||
+ | <h3>Select category</h3> | ||
<div class="category" data-category="synthesis"> | <div class="category" data-category="synthesis"> | ||
<img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" /> | <img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" /> | ||
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May | May | ||
</div> | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - PCR of YFP B4-B4; size: 749 bp <br> | ||
+ | - Ligation YFP B4-B4 with L0-RFP" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/f/f7/T--Humboldt_Berlin--notebook_s_0527.png" | ||
+ | data-category="synthesis" | ||
+ | data-date="05/27 - 06/02"> | ||
+ | June | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - Transformation L0-YFP B4-B4 into <i>E. coli </i><br> | ||
+ | - Colony PCR YFP-B4-B4 in <i>E. coli</i>" | ||
+ | data-category="synthesis" | ||
+ | data-date="06/03 - 06/09"> | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - sequencing result of YFP B4-B4 clone 2 was positive" | ||
+ | data-category="synthesis" | ||
+ | data-date="06/17 - 06/23"> | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="<h2>B2 Linker</h2> | ||
+ | - creating new primers" | ||
+ | data-category="synthesis" | ||
+ | data-date="07/01 - 07/07"> | ||
+ | July | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>B2 Linker</h2> | ||
+ | - Oligo Annealing B2 Linker<br> | ||
+ | - succesfull" | ||
+ | data-category="synthesis" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/e/e8/T--Humboldt_Berlin--notebook_s_0708.png" | ||
+ | data-date="06/17 - 06/23"> | ||
+ | </div> | ||
+ | |||
+ | |||
<!-- human practice --> | <!-- human practice --> | ||
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data-category="chlamy"> | data-category="chlamy"> | ||
January | January | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- First Toxicity-tests in a multicultivator, which measures algal growth under different conditions: Inhibition of C. reinhardtii by ethylene glycol at a concentration of 6 % was confirmed<br> | ||
+ | - Cultivation of strain CC-125 in high salt medium shows inhibited growth <br><br> | ||
+ | - participants: Paul Herrmann, Andrej Andjelic" | ||
+ | data-date="May 2019" | ||
+ | data-category="chlamy"> | ||
+ | May | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- started the transformations of L1-constructs into Chlamy<br> | ||
+ | - checked the functionality of our paromomycin and hygromycin selection cassettes<br> | ||
+ | - Cultivation tests of strain SAG 11-32b in high salt medium with added PET in concentrations of 10-800 mg/L. Wastewater contains 250 mg/L PET before treatment. <br><br> | ||
+ | - participants: Simon Kelterborn, Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann" | ||
+ | data-date="June 2019" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/4/40/T--Humboldt_Berlin--HSM_PET.jpeg" | ||
+ | data-category="chlamy"> | ||
+ | June | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- First Chlamy transformations are discarded, since they were contaminated<br> | ||
+ | - Transformation of further L1-constructs<br> | ||
+ | - constructs containing YFP: colony-PCR showed integration of the plasmid into Chlamy’s genome, but no fluorescence could be measured by the plate reader <br> | ||
+ | - PETase-YFP-containing constructs displayed fluorescence visible under the confocal microscopy<br><br> | ||
+ | - participants: Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann" | ||
+ | data-date="July 2019" | ||
+ | data-category="chlamy"> | ||
+ | July | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- Started with the construction of our own bioreactor. Conceptualization of the functionalities and necessary features had before been experimentally determined during our multicultivation tests<br>" | ||
+ | data-date="August 2019" | ||
+ | data-category="chlamy"> | ||
+ | August | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- Wiring and programming the sensors and LEDs on the DIY-bioreactor<br> | ||
+ | - laser-cutting of the shell and cultivation compartment of our bioreactor" | ||
+ | data-date="September 2019" | ||
+ | data-category="chlamy"> | ||
+ | September | ||
</div> | </div> | ||
</div> | </div> | ||
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function setFixedElements() { | function setFixedElements() { | ||
− | + | var timelineOffset = document.querySelector('.timeline').getBoundingClientRect().top; | |
+ | |||
+ | if (timelineOffset < 150) { | ||
infobox.classList.add('fixed') | infobox.classList.add('fixed') | ||
} else { | } else { | ||
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const activeElements = document.querySelectorAll('.active-timeline-el'); | const activeElements = document.querySelectorAll('.active-timeline-el'); | ||
− | + | var lastActive = activeElements[activeElements.length - 1]; | |
+ | if (lastActive === undefined) { | ||
+ | lastActive = document.querySelectorAll('.timeline > div.visible')[0]; | ||
+ | } | ||
if (lastActive !== undefined) { | if (lastActive !== undefined) { |
Latest revision as of 21:47, 21 October 2019
![notebook](https://static.igem.org/mediawiki/2019/8/81/T--Humboldt_Berlin--Notebook_Header.jpeg)
Notebook
![our stuffed calendar](https://static.igem.org/mediawiki/2019/e/e3/T--Humboldt_Berlin--notebook_final.png)
Select category
![Synthesis: Parts and Vectors](https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png)
![human practice](https://static.igem.org/mediawiki/2019/e/e6/T--Humboldt_Berlin--label_notebook_human_practise.png)
![c. reinhardtii cultivation and transformations](https://static.igem.org/mediawiki/2019/a/ac/T--Humboldt_Berlin--label_notebook_chlamy.png)
October
2018
2018
November
December
January
February
March
May
June
July
January 2019
May
June
July
October
November 2018
December
January
May
June
July
August
September