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{{Humboldt_Berlin}} | {{Humboldt_Berlin}} | ||
<html> | <html> | ||
+ | |||
<div class="has-animations"> | <div class="has-animations"> | ||
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<div class="submenu"> | <div class="submenu"> | ||
<a href="/Team:Humboldt_Berlin/Parts">Parts overview</a> | <a href="/Team:Humboldt_Berlin/Parts">Parts overview</a> | ||
+ | <a href="/Team:Humboldt_Berlin/Basic_Part">Basic parts</a> | ||
+ | <a href="/Team:Humboldt_Berlin/Composite_Part">Composite parts</a> | ||
<a href="/Team:Humboldt_Berlin/Part_Collection">Parts collection</a> | <a href="/Team:Humboldt_Berlin/Part_Collection">Parts collection</a> | ||
</div> | </div> | ||
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<div class="fixed-header-container"> | <div class="fixed-header-container"> | ||
− | + | <section class="fixed-image-header"> | |
− | + | <img src="https://static.igem.org/mediawiki/2019/8/81/T--Humboldt_Berlin--Notebook_Header.jpeg" alt="notebook" /> | |
− | + | </section> | |
− | + | <h1 class="page-headline description">Notebook</h1> | |
− | + | </div> | |
− | + | <div class="full-height fixed-header-content notebook"> | |
− | + | <div class="width-limit padding-container" style="margin: 50px 0;"> | |
− | + | <img src="https://static.igem.org/mediawiki/2019/e/e3/T--Humboldt_Berlin--notebook_final.png" alt="our stuffed calendar"/></br> | |
− | + | </div> | |
+ | <section class="width-limit"> | ||
+ | <div class="category-container"> | ||
+ | <h3>Select category</h3> | ||
<div class="category" data-category="synthesis"> | <div class="category" data-category="synthesis"> | ||
<img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" /> | <img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" /> | ||
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May | May | ||
</div> | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - PCR of YFP B4-B4; size: 749 bp <br> | ||
+ | - Ligation YFP B4-B4 with L0-RFP" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/f/f7/T--Humboldt_Berlin--notebook_s_0527.png" | ||
+ | data-category="synthesis" | ||
+ | data-date="05/27 - 06/02"> | ||
+ | June | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - Transformation L0-YFP B4-B4 into <i>E. coli </i><br> | ||
+ | - Colony PCR YFP-B4-B4 in <i>E. coli</i>" | ||
+ | data-category="synthesis" | ||
+ | data-date="06/03 - 06/09"> | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>YFP</h2> | ||
+ | - sequencing result of YFP B4-B4 clone 2 was positive" | ||
+ | data-category="synthesis" | ||
+ | data-date="06/17 - 06/23"> | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="<h2>B2 Linker</h2> | ||
+ | - creating new primers" | ||
+ | data-category="synthesis" | ||
+ | data-date="07/01 - 07/07"> | ||
+ | July | ||
+ | </div> | ||
+ | <div class="timeline-dot" | ||
+ | data-text="<h2>B2 Linker</h2> | ||
+ | - Oligo Annealing B2 Linker<br> | ||
+ | - succesfull" | ||
+ | data-category="synthesis" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/e/e8/T--Humboldt_Berlin--notebook_s_0708.png" | ||
+ | data-date="06/17 - 06/23"> | ||
+ | </div> | ||
+ | |||
+ | |||
<!-- human practice --> | <!-- human practice --> | ||
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data-category="chlamy"> | data-category="chlamy"> | ||
January | January | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- First Toxicity-tests in a multicultivator, which measures algal growth under different conditions: Inhibition of C. reinhardtii by ethylene glycol at a concentration of 6 % was confirmed<br> | ||
+ | - Cultivation of strain CC-125 in high salt medium shows inhibited growth <br><br> | ||
+ | - participants: Paul Herrmann, Andrej Andjelic" | ||
+ | data-date="May 2019" | ||
+ | data-category="chlamy"> | ||
+ | May | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- started the transformations of L1-constructs into Chlamy<br> | ||
+ | - checked the functionality of our paromomycin and hygromycin selection cassettes<br> | ||
+ | - Cultivation tests of strain SAG 11-32b in high salt medium with added PET in concentrations of 10-800 mg/L. Wastewater contains 250 mg/L PET before treatment. <br><br> | ||
+ | - participants: Simon Kelterborn, Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann" | ||
+ | data-date="June 2019" | ||
+ | data-image="https://static.igem.org/mediawiki/2019/4/40/T--Humboldt_Berlin--HSM_PET.jpeg" | ||
+ | data-category="chlamy"> | ||
+ | June | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- First Chlamy transformations are discarded, since they were contaminated<br> | ||
+ | - Transformation of further L1-constructs<br> | ||
+ | - constructs containing YFP: colony-PCR showed integration of the plasmid into Chlamy’s genome, but no fluorescence could be measured by the plate reader <br> | ||
+ | - PETase-YFP-containing constructs displayed fluorescence visible under the confocal microscopy<br><br> | ||
+ | - participants: Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann" | ||
+ | data-date="July 2019" | ||
+ | data-category="chlamy"> | ||
+ | July | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- Started with the construction of our own bioreactor. Conceptualization of the functionalities and necessary features had before been experimentally determined during our multicultivation tests<br>" | ||
+ | data-date="August 2019" | ||
+ | data-category="chlamy"> | ||
+ | August | ||
+ | </div> | ||
+ | <div class="timeline-month" | ||
+ | data-text="- Wiring and programming the sensors and LEDs on the DIY-bioreactor<br> | ||
+ | - laser-cutting of the shell and cultivation compartment of our bioreactor" | ||
+ | data-date="September 2019" | ||
+ | data-category="chlamy"> | ||
+ | September | ||
</div> | </div> | ||
</div> | </div> | ||
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function setFixedElements() { | function setFixedElements() { | ||
− | + | var timelineOffset = document.querySelector('.timeline').getBoundingClientRect().top; | |
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+ | if (timelineOffset < 150) { | ||
infobox.classList.add('fixed') | infobox.classList.add('fixed') | ||
} else { | } else { | ||
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const activeElements = document.querySelectorAll('.active-timeline-el'); | const activeElements = document.querySelectorAll('.active-timeline-el'); | ||
− | + | var lastActive = activeElements[activeElements.length - 1]; | |
+ | if (lastActive === undefined) { | ||
+ | lastActive = document.querySelectorAll('.timeline > div.visible')[0]; | ||
+ | } | ||
if (lastActive !== undefined) { | if (lastActive !== undefined) { |
Latest revision as of 21:47, 21 October 2019
Notebook
Select category
October
2018
2018
November
December
January
February
March
May
June
July
January 2019
May
June
July
October
November 2018
December
January
May
June
July
August
September