Difference between revisions of "Team:Humboldt Berlin/Notebook"

 
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         <div class="fixed-header-container">
 
         <div class="fixed-header-container">
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            <section class="fixed-image-header">
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          <section class="fixed-image-header">
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                <img src="https://static.igem.org/mediawiki/2019/8/81/T--Humboldt_Berlin--Notebook_Header.jpeg" alt="notebook" />
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              <img src="https://static.igem.org/mediawiki/2019/8/81/T--Humboldt_Berlin--Notebook_Header.jpeg" alt="notebook" />
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            </section>
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          </section>
  
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            <h1 class="page-headline description">Notebook</h1>
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          <h1 class="page-headline description">Notebook</h1>
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        </div>
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      </div>
  
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        <div class="full-height fixed-header-content notebook">
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      <div class="full-height fixed-header-content notebook">
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            <section class="width-limit">
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<div class="width-limit padding-container" style="margin: 50px 0;">
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                <div class="category-container">
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<img src="https://static.igem.org/mediawiki/2019/e/e3/T--Humboldt_Berlin--notebook_final.png" alt="our stuffed calendar"/></br>
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                    <h3>Select category</h3>
+
</div>
 +
          <section class="width-limit">
 +
              <div class="category-container">
 +
                  <h3>Select category</h3>
 
                     <div class="category" data-category="synthesis">
 
                     <div class="category" data-category="synthesis">
 
                         <img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" />
 
                         <img src="https://static.igem.org/mediawiki/2019/6/6f/T--Humboldt_Berlin--label_notebook_synthesis.png" alt="Synthesis: Parts and Vectors" />
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                         - Research on PETase degradation <br>
 
                         - Research on PETase degradation <br>
 
                         - Planning construction design for Golden Gate cloning using 10 different fusion sites according to Patron <br>
 
                         - Planning construction design for Golden Gate cloning using 10 different fusion sites according to Patron <br>
−
                         - Designing Primers for C. reinhardtii specified promotor PsaD containing BpiI recognition site with MoClo fusion sites and fusion sites compatible for L0 backbone. Primers containing mutation to delete BpiI site (Primer 5,6,11-13)."
+
                         - Designing Primers for <i>C. reinhardtii</i> specified promotor PsaD containing BpiI recognition site with MoClo fusion sites and fusion sites compatible for L0 backbone. Primers containing mutation to delete BpiI site (Primer 5,6,11-13)."
 
                         data-date="10/08 - 10/14"
 
                         data-date="10/08 - 10/14"
 
                         data-category="synthesis">
 
                         data-category="synthesis">
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                     </div>
 
                     </div>
 
                     <div class="timeline-dot"
 
                     <div class="timeline-dot"
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                         data-text="- Research on C. reinhardtii cultivation <br>
+
                         data-text="- Research on <i>C. reinhardtii</i>  cultivation <br>
 
                         <h2>YFP</h2>
 
                         <h2>YFP</h2>
 
                         - Designing Primers for YFP with B5-B5 fusion sites taken from AG Hegemann plasmid p135 (Primer 7+8)"
 
                         - Designing Primers for YFP with B5-B5 fusion sites taken from AG Hegemann plasmid p135 (Primer 7+8)"
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                         - overnight cultures of DH10B with L0-PsaD A1/A3 and L0-PsaD A1/B1
 
                         - overnight cultures of DH10B with L0-PsaD A1/A3 and L0-PsaD A1/B1
 
                         <div style='text-indent:20px;'>- plasmid prep of ON-culture</div>
 
                         <div style='text-indent:20px;'>- plasmid prep of ON-culture</div>
−
                         <div style='text-indent:20px;'>- Nano-Drob concentration</div>
+
                         <div style='text-indent:20px;'>- Nano-Drop concentration</div>
 
                         - sequencing result: positive sequencing result of 1 clone each"
 
                         - sequencing result: positive sequencing result of 1 clone each"
 
                         data-date="11/26 - 12/02"
 
                         data-date="11/26 - 12/02"
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                         data-text="<div class='two-columns'><div>
 
                         data-text="<div class='two-columns'><div>
 
                             <h2>Paromomycin</h2>
 
                             <h2>Paromomycin</h2>
−
                             - Preparation of 3 overnight cultures of clone 1,2,3 (colony PCR 22.02.) <br>
+
                             - Preparation of 3 overnight cultures of clones 1, 2, 3 (colony PCR 22.02.) <br>
−
                             -- Plasmid prep the next day<br>
+
                             - Plasmid prep the next day<br>
−
                             -- Sending plasmids for sequencing <br>
+
                             - Sending plasmids for sequencing <br>
 
                             - Corrected sequencing results of L0-Paro B3-B4
 
                             - Corrected sequencing results of L0-Paro B3-B4
 
                             <h2>Hygromycin</h2>
 
                             <h2>Hygromycin</h2>
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                         <div>
 
                         <div>
 
                             <h2>L1a,b,c - RFP</h2>
 
                             <h2>L1a,b,c - RFP</h2>
−
                             - mistake: it was noticed that primer to amplify 3 different RFP versions were designed wrong → buffer bases were missing, so that the restriction enzymes did not attached to the DNA <br>
+
                             - mistake: it was noticed that primer to amplify 3 different RFP versions were designed wrong → buffer bases were missing, so that the restriction enzymes did not attach to the DNA <br>
 
                             - designing and ordering new primers containing buffer bases (primer 84-89)
 
                             - designing and ordering new primers containing buffer bases (primer 84-89)
 
                         </div></div>"
 
                         </div></div>"
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                         May
 
                         May
 
                     </div>
 
                     </div>
 +
<div class="timeline-month"
 +
                        data-text="<h2>YFP</h2>
 +
                        - PCR of YFP B4-B4; size: 749 bp <br>
 +
                        - Ligation YFP B4-B4 with L0-RFP"
 +
                        data-image="https://static.igem.org/mediawiki/2019/f/f7/T--Humboldt_Berlin--notebook_s_0527.png"
 +
                        data-category="synthesis"
 +
                        data-date="05/27 - 06/02">
 +
                        June
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>YFP</h2>
 +
                        - Transformation L0-YFP B4-B4 into <i>E. coli </i><br>
 +
                        - Colony PCR YFP-B4-B4 in <i>E. coli</i>"
 +
                        data-category="synthesis"
 +
                        data-date="06/03 - 06/09">
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>YFP</h2>
 +
                        - sequencing result of YFP B4-B4 clone 2 was positive"
 +
                        data-category="synthesis"
 +
                        data-date="06/17 - 06/23">
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="<h2>B2 Linker</h2>
 +
                        - creating new primers"
 +
                        data-category="synthesis"
 +
                        data-date="07/01 - 07/07">
 +
                        July
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>B2 Linker</h2>
 +
                        - Oligo Annealing B2 Linker<br>
 +
                        - succesfull"
 +
                        data-category="synthesis"
 +
                        data-image="https://static.igem.org/mediawiki/2019/e/e8/T--Humboldt_Berlin--notebook_s_0708.png"
 +
                        data-date="06/17 - 06/23">
 +
                    </div>
 +
 +
  
 
                     <!-- human practice -->
 
                     <!-- human practice -->
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                         - participants: Fabienne, Juli, Andrej, Dimitri"
 
                         - participants: Fabienne, Juli, Andrej, Dimitri"
 
                         data-date="May 2019"
 
                         data-date="May 2019"
−
                         data-category="human-practice">
+
                         data-category="human-practice"
 +
                        data-image="https://static.igem.org/mediawiki/2019/2/24/T--Humboldt_Berlin--notebook_h_May19.png">
 
                         May
 
                         May
 
                     </div>
 
                     </div>
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                         data-category="chlamy">
 
                         data-category="chlamy">
 
                         January
 
                         January
 +
                    </div>
 +
<div class="timeline-month"
 +
                        data-text="- First Toxicity-tests in a multicultivator, which measures algal growth under different conditions: Inhibition of C. reinhardtii by ethylene glycol at a concentration of 6 % was confirmed<br>
 +
                        - Cultivation of strain CC-125 in high salt medium shows inhibited growth <br><br>
 +
                        - participants: Paul Herrmann, Andrej Andjelic"
 +
                        data-date="May 2019"
 +
                        data-category="chlamy">
 +
                        May
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- started the transformations of L1-constructs into Chlamy<br>
 +
                        - checked the functionality of our paromomycin and hygromycin selection cassettes<br>
 +
                        - Cultivation tests of strain SAG 11-32b in high salt medium with added PET in concentrations of 10-800 mg/L. Wastewater contains 250 mg/L PET before treatment. <br><br>
 +
                        - participants: Simon Kelterborn, Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann"
 +
                        data-date="June 2019"
 +
                        data-image="https://static.igem.org/mediawiki/2019/4/40/T--Humboldt_Berlin--HSM_PET.jpeg"
 +
                        data-category="chlamy">
 +
                        June
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- First Chlamy transformations are discarded, since they were contaminated<br>
 +
                        - Transformation of further L1-constructs<br>
 +
                        - constructs containing YFP: colony-PCR showed integration of the plasmid into Chlamy’s genome, but no fluorescence could be measured by the plate reader <br>
 +
                        - PETase-YFP-containing constructs displayed fluorescence visible under the confocal microscopy<br><br>
 +
                        - participants: Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann"
 +
                        data-date="July 2019"
 +
                        data-category="chlamy">
 +
                        July
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- Started with the construction of our own bioreactor. Conceptualization of the functionalities and necessary features had before been experimentally determined during our multicultivation tests<br>"
 +
                        data-date="August 2019"
 +
                        data-category="chlamy">
 +
                        August
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- Wiring and programming the sensors and LEDs on the DIY-bioreactor<br>
 +
                        - laser-cutting of the shell and cultivation compartment of our bioreactor"
 +
                        data-date="September 2019"
 +
                        data-category="chlamy">
 +
                        September
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
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Latest revision as of 21:47, 21 October 2019

notebook

Notebook

our stuffed calendar

Select category

Synthesis: Parts and Vectors
human practice
c. reinhardtii cultivation and transformations
October
2018
November
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January
February
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June
July
January 2019
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June
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November 2018
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