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| </div> | | </div> |
| <div class="devider"></div> | | <div class="devider"></div> |
− | <a href="https://igem.org/2019_Judging_Form?team=Humboldt_Berlin"> | + | <a href="/Team:Humboldt_Berlin/Achievements"> |
| For Judges | | For Judges |
| </a> | | </a> |
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| <h3 class="headline3">Supporters</h3> | | <h3 class="headline3">Supporters</h3> |
| <p class="block-text medium-sized"> | | <p class="block-text medium-sized"> |
− | Our team accomplished great things in the project <i>Chlamylicious</i> over the course of this year’s iGEM competition: We adapted a toolkit for the green algae <i>C. reinhardtii</i> with parts for successful and easy cloning of any transcription unit, which we implemented for the expression of the PET-degrading enzyme PETase by the algae and designed a bioreactor for its cultivation under constant monitoring of all parameters. These were our greatest supporters: | + | Our team accomplished great things in the project <i>Chlamylicious</i> over the course of this year’s iGEM competition: We adapted a toolkit for the green alga <i>C. reinhardtii</i> with parts for successful and easy cloning of any transcription unit, which we implemented for the expression of the PET-degrading enzyme PETase by the alga and designed a bioreactor for its cultivation under constant monitoring of all parameters. These were our greatest supporters: |
| </br> | | </br> |
| <ul style="font-size:18px"> | | <ul style="font-size:18px"> |
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| <LI>our supervisors <b>Prof. Dr. Schmitz-Linneweber and Prof. Dr. Hegemann</b></li> | | <LI>our supervisors <b>Prof. Dr. Schmitz-Linneweber and Prof. Dr. Hegemann</b></li> |
| <LI>the principal investigator <b>Simon Kelterborn</b>, who guided us with his expertise in transformations of <i>Chlamydomonas</i> and CRISPR-aided genetic engineering</li> | | <LI>the principal investigator <b>Simon Kelterborn</b>, who guided us with his expertise in transformations of <i>Chlamydomonas</i> and CRISPR-aided genetic engineering</li> |
− | <LI>the <b>biological technical assistants</b> Heide (Biophysics lab), Conny, Thea and Irina (all Genetics lab), who enabled us to launch the wet-lab</li> | + | <LI>the <b>biological technical assistants</b> Irina, Heide (Biophysics lab), Conny, Thea and Irina (all Genetics lab), who enabled us to launch the wet-lab</li> |
| <LI>computer scientist <b>Vincent Will</b>, who implemented the design for our website</li> | | <LI>computer scientist <b>Vincent Will</b>, who implemented the design for our website</li> |
− | <LI><b>Dr. Ralf Steuer</b>, who helped us developing the bioreactor</li> | + | <LI><b>Dr. Ralf Steuer</b>, who helped us developing the bioreactor</li> |
| <LI><b>Prof. Dr. Ringrose</b>, who coached us on how to create a good scientific poster</li> | | <LI><b>Prof. Dr. Ringrose</b>, who coached us on how to create a good scientific poster</li> |
− | <LI>electrical engineer <b>Markus Schulz</b>, who helped designing and wiring the electronics for our bioreactor</li> | + | <LI>electronic engineer <b>Markus Schulz</b>, who helped designing and wiring the electronics for our bioreactor</li> |
| <LI>technical engineering student <b>Alejandro Gómez Jiménez</b> from the Technical University of Berlin, who made the CAD-models for all parts of our bioreactor</li> | | <LI>technical engineering student <b>Alejandro Gómez Jiménez</b> from the Technical University of Berlin, who made the CAD-models for all parts of our bioreactor</li> |
| <LI>the Interdisciplinary Workshop <b>„Matters of Activity“</b> of Humboldt University, who laser-cut our hardware</li> | | <LI>the Interdisciplinary Workshop <b>„Matters of Activity“</b> of Humboldt University, who laser-cut our hardware</li> |
| <LI>the <b>Mechanical Workshop</b> of the Physics Department of our university</li> | | <LI>the <b>Mechanical Workshop</b> of the Physics Department of our university</li> |
| + | <LI><b>Dr. Nina Siragusa</b> from Merck KGaA, who presented us to her broad scientific network and supported our outreach</li> |
| + | <LI><b>Arne Kuczmera</b> from the Berliner Wasserbetriebe, who supported our research at the waste water treatment plant</li> |
| </ul> | | </ul> |
| </p> | | </p> |
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| <!--------------------------------------- TWO COLUMN IMG LEFT END -----------------------------------------------></br></br> | | <!--------------------------------------- TWO COLUMN IMG LEFT END -----------------------------------------------></br></br> |
| <div class="width-limit padding-container" style="width: 70%; margin-left:auto;margin-right:auto"> | | <div class="width-limit padding-container" style="width: 70%; margin-left:auto;margin-right:auto"> |
− | <p><b>Re-used parts from different sources</b></p> | + | <h3 class="headline3">Re-used parts from different sources</h3> |
| <table> | | <table> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">AR</td> | | <td style="padding-left: 15px">AR</td> |
− | <td>Plasmid p114, Hegemann lab (Biophysics Department, Humboldt University of Berlin)</td> | + | <td>Plasmid p114, Hegemann lab (Biophysics Department, Humboldt Universität zu Berlin)</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td style="padding-left: 15px">ARS</td> | | <td style="padding-left: 15px">ARS</td> |
− | <td>Ordered oligos from IDT</br>Sequence:https://www.uniprot.org/blast/?about=A8ISJ6[1-26]&key=Signal%20peptide </td> | + | <td>Ordered oligos from IDT</br>Sequence: A8ISJ6[1-26] <i>(Uniprot)</i> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td style="padding-left: 15px">AmpOri </td> | | <td style="padding-left: 15px">AmpOri </td> |
− | <td>Amplified from MoClo plasmid <a href="https://www.addgene.org/48000/">pICH47732</a></td> | + | <td>Amplified from MoClo plasmid pICH47732 <i>(Addgene)</i></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">GLE </td> | | <td style="padding-left: 15px">GLE </td> |
− | <td><a href="https://www.uniprot.org/blast/?about=Q93WN9[1-28]&key=Signal%20peptide">Uniprot Q93WN9[1 - 28]</a></td> | + | <td>Sequence: Q93WN9[1 - 28] <i>(Uniprot)</i> </td> |
| </tr> | | </tr> |
− | <tr> | + | <tr> |
| <td style="padding-left: 15px">3xHA-Tag</td> | | <td style="padding-left: 15px">3xHA-Tag</td> |
| <td>Ordered oligos from IDT</td> | | <td>Ordered oligos from IDT</td> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">Hyg</td> | | <td style="padding-left: 15px">Hyg</td> |
− | <td>Amplified from p360, Hegemann lab (Biophysics Department, Humboldt University of Berlin)</td> | + | <td>Amplified from p360, Hegemann lab (Biophysics Department, Humboldt Universität zu Berlin)</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">Paro</td> | | <td style="padding-left: 15px">Paro</td> |
− | <td> Amplified from p114, Hegemann lab (Biophysics Department, Humboldt University of Berlin) | + | <td> Amplified from p114, Hegemann lab (Biophysics Department, Humboldt Universität zu Berlin) |
| </td> | | </td> |
| </tr> | | </tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">PETase (<i>E. coli</i>)</td> | | <td style="padding-left: 15px">PETase (<i>E. coli</i>)</td> |
− | <td> <a href="https://www.addgene.org/112203/ | + | <td> Adgene's plasmid: plasmid #112203</td> |
− | ">Adgene's plasmid: plasmid #112203</a></td>
| + | |
| </td> | | </td> |
| </tr> | | </tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">PtxD</td> | | <td style="padding-left: 15px">PtxD</td> |
− | <td> Sequence: <a href="https://www.ncbi.nlm.nih.gov/nuccore/AF061070">AF061070.1</a></br>Ordered from GeneCust | + | <td> Sequence: AF061070.1 <i>(ncbi)</i></br>Ordered from GeneCust |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td style="padding-left: 15px">PsaD</td> | | <td style="padding-left: 15px">PsaD</td> |
− | <td>Plasmid 51, Hegemann lab (Biophysics Department, Humboldt University of Berlin)</td> | + | <td>Plasmid 51, Hegemann lab (Biophysics Department, Humboldt Universität zu Berlin)</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td style="padding-left: 15px">RFP</td> | | <td style="padding-left: 15px">RFP</td> |
− | <td>p42, Hegemann lab (Biophysics Department, Humboldt University of Berlin)</td> | + | <td>p42, Hegemann lab (Biophysics Department, Humboldt Universität zu Berlin)</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td style="padding-left: 15px">Spec</td> | | <td style="padding-left: 15px">Spec</td> |
− | <td>Amplified from an Addgene MoClo Plasmid <a href="https://www.addgene.org/51833/">pAGM9121</a></td> | + | <td>Amplified from the MoClo Plasmid pAGM9121 <i>(Addgene)</i></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td style="padding-left: 15px">YFP</td> | | <td style="padding-left: 15px">YFP</td> |
| <td>Amplified from YFP Sequenz of p135 pCr3-Ble2-YFP-Sp-APH plasmid | | <td>Amplified from YFP Sequenz of p135 pCr3-Ble2-YFP-Sp-APH plasmid |
− | >pAGM9121</br>Overhang PCR: Amplification</a></td>
| + | pAGM9121</br>Overhang PCR: Amplification</a></td> |
| </tr> | | </tr> |
| </table> | | </table> |
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| <LI>Research more on the possibilities of <i>C. reinhardtii</i> as a SynBio tool and familiarize ourselves with project management conducts from <b>July-October 2018</b>. </li> | | <LI>Research more on the possibilities of <i>C. reinhardtii</i> as a SynBio tool and familiarize ourselves with project management conducts from <b>July-October 2018</b>. </li> |
| <LI>Start fundraising initiatives in <b>July 2018</b>. </li> | | <LI>Start fundraising initiatives in <b>July 2018</b>. </li> |
− | <LI>Get part of the team started with working in the wet lab in <b>November 2018</b>.</li> | + | <LI>Get part of the team started with working in the wet lab in <b>October 2018</b>.</li> |
| <LI>Organize Human Practice events and possibilities of outreach to the broader public from <b>November 2018 - October 2019</b>.</li> | | <LI>Organize Human Practice events and possibilities of outreach to the broader public from <b>November 2018 - October 2019</b>.</li> |
| <LI>Get a second lab team to finish the wet lab work from <b>April-October 2019</b>.</li> | | <LI>Get a second lab team to finish the wet lab work from <b>April-October 2019</b>.</li> |
| + | <LI>Conceptualize a bioreactor for optimal cultivation and build it from <b>June-October 2019</b>.</li> |
| </UL> | | </UL> |
| </p> | | </p> |
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| <!--------------------------------------- TWO COLUMN IMG RIGHT END ------------------------------------------------> | | <!--------------------------------------- TWO COLUMN IMG RIGHT END ------------------------------------------------> |
| + | |
| + | <!--------------------------------------- TO TOP LINK -----------------------------------------------> |
| + | </br> |
| + | <a href="#" class="to-top-link"> |
| + | <img src="https://static.igem.org/mediawiki/2019/3/3e/T--Humboldt_Berlin--ArrowDown.jpg" /> Go to top |
| + | </a> |
| + | <!--------------------------------------- TO TOP LINK END -------------------------------------------> |
| + | |
| | | |
| </section> | | </section> |