Difference between revisions of "Team:ECUST China/Notebook"
| Line 48: | Line 48: | ||
<p>We firstly attempted to construct the cex and cenA on the pET-28b separately, since the dual inverter plasmid has not been constructed and the pET-28b is a reliable plasmid to express heterologous protein.</p> | <p>We firstly attempted to construct the cex and cenA on the pET-28b separately, since the dual inverter plasmid has not been constructed and the pET-28b is a reliable plasmid to express heterologous protein.</p> | ||
<p>At the beginning, we got desired parts from kit by PCR very easily. And through Gibson-assembly method, we ligated the parts we purified. When we tested the ligation section by test primer and first generation sequencing, nonetheless, there were not a single clone comprise all the fragments we expected to be ligated.(More details please click the botton below)</p> | <p>At the beginning, we got desired parts from kit by PCR very easily. And through Gibson-assembly method, we ligated the parts we purified. When we tested the ligation section by test primer and first generation sequencing, nonetheless, there were not a single clone comprise all the fragments we expected to be ligated.(More details please click the botton below)</p> | ||
| − | <div class="btn"><a href="https://static.igem.org/mediawiki/2019/8/8d/T--ECUST_China--experiments_protocols.pdf"> <b style="color: white;">Protocols</b></a></div> | + | <div class="btn"><a href="https://static.igem.org/mediawiki/2019/8/8d/T--ECUST_China--experiments_protocols.pdf" style="padding-left:16px;"> <b style="color: white;">Protocols</b></a></div> |
</li> | </li> | ||
<li> | <li> | ||
| Line 98: | Line 98: | ||
</ul> | </ul> | ||
<p>More details about our notebook please click</p> | <p>More details about our notebook please click</p> | ||
| − | <div class="btn"><a href="https://static.igem.org/mediawiki/2019/a/ac/T--ECUST_China--notebook.pdf"> <b>Notebook</b></a></div> | + | <div class="btn"><a href="https://static.igem.org/mediawiki/2019/a/ac/T--ECUST_China--notebook.pdf" style="padding-left:16px;"> <b style="color:white;">Notebook</b></a></div> |
</div></div></div> | </div></div></div> | ||
<br><br><br><br> | <br><br><br><br> | ||
Revision as of 19:33, 21 October 2019
- 01 Introduction
- 02 Progress
Introduction
Paper Transformer is a superhero we made to change the paper world, and several of team members devoted most of their time and energy this season.
We assigned four iGEMers to achieve three abilities of our Paper Transformer. In detail, we assigned Hu Shijia for cellulose degrading module, Xiang Lan for cellulose producing module, Miao Zongjie and Jin Qianwei for inverter module.
Progress
—January 2019
The senior students publicized the iGEM and recruited candidates aspiring to participate iGEM representing ECUST-2019 iGEM team.
—February 2019
We had a brain storm within the iGEM club of ECUST.
—April 2019
We participated a meetup held at New York University Shanghai.
—May 2019
We decided to focus on Paper Transformer as our project out of three others.
We were invited to Jiangnan University in Wuxi, Jiangsu province to discuss our project. We communicated with three teams of Jiangnan University on human practices and gene circuit design.
—June 2019
We started to plan our wet lab arrangement and construction work.
—July 2019
Our experiment didn’t start until this month, but we quickly assigned the tasks into several team member. In detail, shows as follows:
- Cellulose degrading ability
This part was mostly undertaken by Hushijia, and later assisted by Xianglan. The results could be seen at here
. We firstly attempted to construct the cex and cenA on the pET-28b separately, since the dual inverter plasmid has not been constructed and the pET-28b is a reliable plasmid to express heterologous protein.
At the beginning, we got desired parts from kit by PCR very easily. And through Gibson-assembly method, we ligated the parts we purified. When we tested the ligation section by test primer and first generation sequencing, nonetheless, there were not a single clone comprise all the fragments we expected to be ligated.(More details please click the botton below)
-
Cellulose producing ability
This part was firstly undertaken by Xianglan, and then taken over by Jinqianwei and Miaozongjie. The results could be seen at here
.We tried to purchase Gluconacetobacter xylinus from
-
Inversion-control ability
This part was mostly undertaken by Jinqianwei and Miaozongjie. The results could be seen at here
.
—August 2019
Cellulose degrading ability
that
Cellulose producing ability
that
Inversion-control ability
that
—September 2019
Cellulose degrading ability
that
Cellulose producing ability
that
Inversion-control ability
that
—October 2019
Cellulose degrading ability
that
Cellulose producing ability
that
Inversion-control ability
that
More details about our notebook please click
ECUST_China
EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Shanghai, China
GET IN TOUCH
+86 021-64253306
ecust_igem_2019@163.com
