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(17 intermediate revisions by 2 users not shown) |
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| <!-- HEADLINE TEXT --> | | <!-- HEADLINE TEXT --> |
− | <h1 class="page-headline description" style="color:#ffffff">Basic Part</h1> | + | <h1 class="page-headline description" style="color:#ffffff">Basic Parts</h1> |
| </div> | | </div> |
| | | |
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| <div class="width-limit padding-container ptable" style="width: 60%; margin-left:auto;margin-right:auto"> | | <div class="width-limit padding-container ptable" style="width: 60%; margin-left:auto;margin-right:auto"> |
| <p><table> | | <p><table> |
| + | <!------------------------------------------ Basic L0 parts ----------------------------------------> |
| + | |
| | | |
| <tr> | | <tr> |
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| <tr> | | <tr> |
| <td>L0-Paro</td> | | <td>L0-Paro</td> |
− | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K29840006">BBa_K2984006 </a> </td> | + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984006">BBa_K2984006 </a> </td> |
| <td>Coding</td> | | <td>Coding</td> |
| <td>L0 backbone with Paromomycine resistance CDS </td> | | <td>L0 backbone with Paromomycine resistance CDS </td> |
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| <td>L0 backbone with PsaD promoter </td> | | <td>L0 backbone with PsaD promoter </td> |
| <td>A1-B1</td> | | <td>A1-B1</td> |
− | <td style="color:#0A2187;text-align:center">▇</td<!blau> | + | <td style="color:#1AA67A;text-align:center">▇</td<!grün> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with AR promoter </td> | | <td>L0 backbone with AR promoter </td> |
| <td>A1-A3</td> | | <td>A1-A3</td> |
− | <td style="color:#FFD185;text-align:center">▇</td><!gelb> | + | <td style="color:#1AA67A;text-align:center">▇</td><!grün> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone (plasmid with RFP insert) </td> | | <td>L0 backbone (plasmid with RFP insert) </td> |
| <td>-</td> | | <td>-</td> |
− | <td style="color:#1AA67A;text-align:center">▇</td><!gürn> | + | <td>-</td> |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
| <td>L0-PETase</td> | | <td>L0-PETase</td> |
− | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K29840011">BBa_K2984011 </a> </td> | + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">BBa_K2984011 </a> </td> |
| <td>Coding</td> | | <td>Coding</td> |
| <td>L0 backbone with PETase CDS </td> | | <td>L0 backbone with PETase CDS </td> |
| <td>B3-B4</td> | | <td>B3-B4</td> |
− | <td>-</td> | + | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
| <td>L0-Hyg</td> | | <td>L0-Hyg</td> |
− | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K29840012">BBa_K2984012 </a> </td> | + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984012">BBa_K2984012 </a> </td> |
| <td>Coding</td> | | <td>Coding</td> |
| <td>L0 backbone with Hygromycin B resistance CDS </td> | | <td>L0 backbone with Hygromycin B resistance CDS </td> |
| <td>B3-B4</td> | | <td>B3-B4</td> |
− | <td>-</td> | + | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with phosphite oxidoreductase CDS </td> | | <td>L0 backbone with phosphite oxidoreductase CDS </td> |
| <td>B3-B4</td> | | <td>B3-B4</td> |
− | <td>-</td> | + | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with 6x histidine tag</td> | | <td>L0 backbone with 6x histidine tag</td> |
| <td>B5-B5</td> | | <td>B5-B5</td> |
− | <td>-</td> | + | <td style="color:#A6C2ED;text-align:center">▇</td><!hellblau> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with 3x histidine tag</td> | | <td>L0 backbone with 3x histidine tag</td> |
| <td>B5-B5</td> | | <td>B5-B5</td> |
− | <td>-</td> | + | <td style="color:#A6C2ED;text-align:center">▇</td><!hellblau> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with yellow fluorescent protein CDS </td> | | <td>L0 backbone with yellow fluorescent protein CDS </td> |
| <td>B3-B4</td> | | <td>B3-B4</td> |
− | <td>-</td> | + | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with RuBisCo terminator </td> | | <td>L0 backbone with RuBisCo terminator </td> |
| <td>B5-C1</td> | | <td>B5-C1</td> |
− | <td>-</td> | + | <td style="color:#0A2187;text-align:center">▇</td><!dunkelblau> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with RuBisCo terminator </td> | | <td>L0 backbone with RuBisCo terminator </td> |
| <td>B6-C1</td> | | <td>B6-C1</td> |
− | <td>-</td> | + | <td style="color:#0A2187;text-align:center">▇</td><!dunkelblau> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with PsaD promoter </td> | | <td>L0 backbone with PsaD promoter </td> |
| <td>A1-A3</td> | | <td>A1-A3</td> |
− | <td style="color:#A6C2ED;text-align:center">▇</td><!hbl> | + | <td style="color:#1AA67A;text-align:center">▇</td><!grün> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with yellow fluorescent protein CDS </td> | | <td>L0 backbone with yellow fluorescent protein CDS </td> |
| <td>B4-B4</td> | | <td>B4-B4</td> |
− | <td>-</td> | + | <td style="color:#A6C2ED;text-align:center">▇</td><!hbl> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with AR promoter </td> | | <td>L0 backbone with AR promoter </td> |
| <td>A1-B1</td> | | <td>A1-B1</td> |
− | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> | + | <td style="color:#1AA67A;text-align:center">▇</td><!grün> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with phosphite oxidoreductase CDS </td> | | <td>L0 backbone with phosphite oxidoreductase CDS </td> |
| <td>B3-B3</td> | | <td>B3-B3</td> |
− | <td>-</td> | + | <td style="color:#FC4A1F;text-align:center">▇</td><!rot> |
| </tr> | | </tr> |
| | | |
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| <td>L0 backbone with Linker sequence </td> | | <td>L0 backbone with Linker sequence </td> |
| <td>B2-B2</td> | | <td>B2-B2</td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984044">BBa_K2984044</a> </td> | | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984044">BBa_K2984044</a> </td> |
| <td>Intermediate</td> | | <td>Intermediate</td> |
− | <td>L0 backbone with Bleomycin resistance </td> | + | <td>L0 backbone with self-cleaving resistance </td> |
| <td>B1-B1</td> | | <td>B1-B1</td> |
| <td>-</td> | | <td>-</td> |
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| <td>L0 backbone with PsaDIntron</td> | | <td>L0 backbone with PsaDIntron</td> |
| <td> A1-A3</td> | | <td> A1-A3</td> |
− | <td style="color:#1AA67A;text-align:center">▇</td> | + | <td style="color:#1AA67A;text-align:center">▇</td><!grün> |
− | </tr> <tr> | + | </tr> |
| + | <tr> |
| <td>L0-PETase</td> | | <td>L0-PETase</td> |
| <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">BBa_K2984049</a> </td> | | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">BBa_K2984049</a> </td> |
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| <td>Linker L1c -> L0</td> | | <td>Linker L1c -> L0</td> |
| <td> - </td> | | <td> - </td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td>Linker L0 -> L1a</td> | | <td>Linker L0 -> L1a</td> |
| <td> - </td> | | <td> - </td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td>Linker L0 -> L1b</td> | | <td>Linker L0 -> L1b</td> |
| <td> - </td> | | <td> - </td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td>Linker L2 -> L1b</td> | | <td>Linker L2 -> L1b</td> |
| <td> - </td> | | <td> - </td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| | | |
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| <td>Linker sequence</td> | | <td>Linker sequence</td> |
| <td> B2-B2 </td> | | <td> B2-B2 </td> |
− | <td>-</td> | + | <td style="color:#E4DEE7;text-align:center">▇</td><!grau> |
| </tr> | | </tr> |
| | | |
| </table> | | </table> |
− |
| |
| | | |
| </div> | | </div> |
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| <table> | | <table> |
| <tr> | | <tr> |
− | <th scope="col">Promoter</th>
| + | <th scope="col">Promoter</th> |
− | <th scope="col">5' UTR</th>
| + | <th scope="col">5' UTR</th> |
| <th scope="col">CDS</th> | | <th scope="col">CDS</th> |
| <th scope="col">CDS/Tag</th> | | <th scope="col">CDS/Tag</th> |
| <th scope="col">Terminator</th> | | <th scope="col">Terminator</th> |
| + | <th scope="col">Linker</th> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td style="text-align:center">B4/B4</td> | | <td style="text-align:center">B4/B4</td> |
| <td style="text-align:center">B5/C1</br>(B6/C1)</td> | | <td style="text-align:center">B5/C1</br>(B6/C1)</td> |
| + | <td style="text-align:center">-</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| <td style="color:#A6C2ED;text-align:center">▇</td> | | <td style="color:#A6C2ED;text-align:center">▇</td> |
| <td style="color:#0A2187;text-align:center">▇</td> | | <td style="color:#0A2187;text-align:center">▇</td> |
| + | <td style="color:#E4DEE7;text-align:center">▇</td> |
| </tr> | | </tr> |
| </table> | | </table> |
Line 457: |
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| </div> | | </div> |
| <!--------------------------------------- TWO COLUMN IMG LEFT END -----------------------------------------------> | | <!--------------------------------------- TWO COLUMN IMG LEFT END -----------------------------------------------> |
− |
| + | |
| + | |
| + | <!-------------------------------------- GHOST PARTS ------------------------------------------------------------> |
| + | |
| + | <div class="width-limit padding-container"style="width: 60%; margin-left:auto;margin-right:auto"> |
| + | <img src="https://static.igem.org/mediawiki/2019/0/06/T--Humboldt_Berlin--warning_not-moclo.png" style="max-width:50%; margin-left: auto; margin-right: auto;"/> |
| + | </div> |
| + | |
| + | <br><br> |
| + | <div class="width-limit padding-container ptable" style="width: 60%; margin-left:auto;margin-right:auto"> |
| + | <h3 class="headline3">Our ghost (not moclo compatible) parts 👻</h3> |
| + | <table> |
| + | |
| + | |
| + | <tr> |
| + | <th scope="col">Part Name</th> |
| + | <th scope="col">Registry Name </th> |
| + | <th scope="col">Registry Type</th> |
| + | <th scope="col">Description </th> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>L0-ARS1</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984066">BBa_K2984066 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with 3'UTR Arylsulfatase1 secretion signal</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-GLE</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984067">BBa_K2984067 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with 3'UTR gametolysin secretion signal</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-AR</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984068">BBa_K2984068 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with AR promoter</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-PETase</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984069">BBa_K2984069 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with PETase</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-Hyg</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984070">BBa_K2984070 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with Hygromycin B resistance CDS</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-ptxD</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984071">BBa_K2984071 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with phosphite oxidoreductase CDS</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-6xHistag</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984072">BBa_K2984072 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with 6x histidine tag</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-3xHis tag</td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984073">BBa_K2984073 </a> </td> |
| + | <td>Coding</td> |
| + | <td> L0 backbone with 3x histidine tag</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-SP<sub>20</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984074">BBa_K2984074 </a> </td> |
| + | <td>Coding</td> |
| + | <td> L0 backbone with glycomodule for secretion enhancement</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-YFP</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984075">BBa_K2984075 </a> </td> |
| + | <td>Coding</td> |
| + | <td> L0 backbone with yellow fluorescent protein CDS</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-RbcS2</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984076">BBa_K2984076 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with RuBisCo terminator</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-PsaD</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984077">BBa_K2984077 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with PsaD promoter</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-PsaDIntron</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984078">BBa_K2984078 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with PsaDIntron</td> |
| + | </tr> |
| + | <tr> |
| + | <td>L0-Cah</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984079">BBa_K2984079 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with Carboanhyydrase</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td>L0-ble+scp</sub></td> |
| + | <td> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2984090">BBa_K2984090 </a> </td> |
| + | <td>Coding</td> |
| + | <td>L0 backbone with Bleomycin resistance</td> |
| + | </tr> |
| + | </table> |
| + | </div> |
| + | <!--------------------------------------- GHOST PART END -----------------------------------------------> |
| + | |
| + | |
| <!--------------------------------------- TO TOP LINK -----------------------------------------------> | | <!--------------------------------------- TO TOP LINK -----------------------------------------------> |
| <a href="#" class="to-top-link"> | | <a href="#" class="to-top-link"> |
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| </a> | | </a> |
| <!--------------------------------------- TO TOP LINK END -------------------------------------------> | | <!--------------------------------------- TO TOP LINK END -------------------------------------------> |
− | | + | </section> |
| <!------------------------------------------ FOOTER ----------------------------------------> | | <!------------------------------------------ FOOTER ----------------------------------------> |
| <div class="footer-container"> | | <div class="footer-container"> |