Difference between revisions of "Team:OUC-China/Safety"

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            <div style="display:inline;height:45px;color: white;padding:0%;text-decoration:none;position:relative;line-height:33px">PROJECT
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                <div class="nav-sub-item"><a href="https://2019.igem.org/Team:OUC-China/Description" style="color: #969696;text-decoration:none;padding:0;left:20px">Description</a></div>
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                <div class="nav-sub-item"><a href="https://2019.igem.org/Team:OUC-China/Design" style="color: #b1b5b9;text-decoration:none;padding:0;">Design</a></div>
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                <div class="nav-sub-item"><a href="https://2019.igem.org/Team:OUC-China/Results" style="color: #b1b5b9;text-decoration:none;padding:0;">Results</a></div>
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            <div style="display:inline;height:45px;color: white;padding:0%;text-decoration:none;position:relative">LAB WORK
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                <div class="nav-sub-item"><a href="https://2019.igem.org/Team:OUC-China/Basic_Part" style="color: #b1b5b9;text-decoration:none;padding:0;">Basic Parts</a></div>
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            <div style="display:inline;height:45px;color: white;padding:0%;color: white;white-space:nowrap;text-decoration:none;position:relative;" >HP
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                <div class="nav-sub-item"><a href="https://2019.igem.org/Team:OUC-China/Attributions" style="color: #b1b5b9;padding:0;text-decoration:none">Attributions</a></div>
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    <div class="side2" style="line-height:67px;text-decoration:none;color:black;"  href="#kly">Experiment</div>
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<div class='text'>1. Safe project design</div></br>
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<div class='text'>Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5AlphaZ1 is employed as the main chassis. As a matter of fact, when using modular riboswitch to tune the expression of genes, it is harmless for the growth of bacteria and relatively safe for industrialized applications. For the sake of environments, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.</div></br>
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<div class='text'></div></br>
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<div class='text'>2. Safe lab work</div></br>
 +
<div class='text'>We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. </div></br>
 +
<div class='text'> </div></br>
 +
<div class='text'>图 Fig.1 Our laboratory and personal safety precautions</div></br>
 +
<div class='text'>There is no zero risk to the experimenter in any experiment,  hence we are properly equipped with facilities, such as latex gloves, nitrile gloves，goggles, lab coats to protect us from biotic and abiotic hazards.</div></br>
 +
<div class='text'> </div></br>
 +
<div class='text'>图Fig.2 Table for routine inspection</div></br>
 +
<div class='text'>Furthermore, several operational zones are divided scientifically for special experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone where is the recording experimental note and organizing experimental data area, C zone where is a molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis area. For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab. </div></br>
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<div class='text'> </div></br>
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<div class='text'> </div></br>
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<div class='text'>图Fig.3 Safety operation</div></br>
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<div class='text'></div></br>
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<div class='text'>Practical lab safety manual</div></br>
 +
<div class='text'></div></br>
 +
<div class='text'>?  Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.</div></br>
 +
<div class='text'>?  Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.</div></br>
 +
<div class='text'>?  Well prepared experiment plans should be written before you start.</div></br>
 +
<div class='text'>?  Lab coats and gloves must be worn when conducting experiments.</div></br>
 +
<div class='text'>?  Short skirts, shorts, and open shoes must not be worn.</div></br>
 +
<div class='text'>?  If you see a colleague doing something dangerous, point it out to him or her.</div></br>
 +
<div class='text'>?  Lab coats must not be worn outside laboratories and in public areas.</div></br>
 +
<div class='text'>?  Know where safety equipment (eyewash, shower and extinguisher) is located.</div></br>
 +
<div class='text'>?  Food/drink is not allowed in laboratories where chemicals are used stored.</div></br>
 +
<div class='text'></div></br>
 +
<div class='text'>3. Safety Education</div></br>
 +
<div class='text'>OUC-China has built up a WeChat public platform, a worldwide platform with billions of users for documentation, communication and popularization. To engage with the public and help them attach importance to bio-safety,we published some articles. Fortunately, all these articles are rather approachable and most of them received positive feedbacks.</div></br>
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<div class='text'></div></br>
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Revision as of 18:36, 15 October 2019

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1. Safe project design

Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5AlphaZ1 is employed as the main chassis. As a matter of fact, when using modular riboswitch to tune the expression of genes, it is harmless for the growth of bacteria and relatively safe for industrialized applications. For the sake of environments, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.


2. Safe lab work

We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage.


图 Fig.1 Our laboratory and personal safety precautions

There is no zero risk to the experimenter in any experiment, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves，goggles, lab coats to protect us from biotic and abiotic hazards.


图Fig.2 Table for routine inspection

Furthermore, several operational zones are divided scientifically for special experiments like A zone where is weighing the drugs, preparing the medium and sterilizing, B zone where is the recording experimental note and organizing experimental data area, C zone where is a molecular operation area, D zone where is RNA electrophoresis and a polyacrylamide gel electrophoresis area. For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.



图Fig.3 Safety operation


Practical lab safety manual


? Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.

? Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.

? Well prepared experiment plans should be written before you start.

? Lab coats and gloves must be worn when conducting experiments.

? Short skirts, shorts, and open shoes must not be worn.

? If you see a colleague doing something dangerous, point it out to him or her.

? Lab coats must not be worn outside laboratories and in public areas.

? Know where safety equipment (eyewash, shower and extinguisher) is located.

? Food/drink is not allowed in laboratories where chemicals are used stored.


3. Safety Education

OUC-China has built up a WeChat public platform, a worldwide platform with billions of users for documentation, communication and popularization. To engage with the public and help them attach importance to bio-safety,we published some articles. Fortunately, all these articles are rather approachable and most of them received positive feedbacks.