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| <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/7/73/T--Humboldt_Berlin--YFP_fluorescence_intensity.png" alt="fluorescence intensity" /> | | <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/7/73/T--Humboldt_Berlin--YFP_fluorescence_intensity.png" alt="fluorescence intensity" /> |
− | <caption>Fig. - Fluorescence intensity of C. reinhardtii WT and a YFP carrying clone, in decreasing optical density of the cell culture. Excitation at 490 nm and emission measurement at 528 nm.</caption> | + | <caption>Fig. - Fluorescence intensity of C. reinhardtii WT and a YFP carrying clone, in decreasing optical density of the cell culture. Excitation at 490 nm and emission measurement at 528 nm. The results clearly show that the fluorescence of the YFP expressing clone is higher than the autofluorescence of the WT algae.</caption> |
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| <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/5/50/T--Humboldt_Berlin--YFP_fluorescence_difference_spectrum.png" alt="fluorescence difference spectrum" /> | | <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/5/50/T--Humboldt_Berlin--YFP_fluorescence_difference_spectrum.png" alt="fluorescence difference spectrum" /> |
− | <caption>Fig. - YFP emission spectrum of a C. reinhardtii clone with YFP. Difference spectrum of WT and YFP spectra</caption> | + | <caption>Fig. - YFP emission spectrum of a C. reinhardtii clone with YFP with an emission maximum at approximately 530 nm. Difference spectrum of WT and YFP spectra</caption> |
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| <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/e/e5/T--Humboldt_Berlin--Psad-YFP-syn%28FL_OD%29-1day.png" alt="fluorescence intensity time resolved" /> | | <img class="is-revealing" src="https://static.igem.org/mediawiki/2019/e/e5/T--Humboldt_Berlin--Psad-YFP-syn%28FL_OD%29-1day.png" alt="fluorescence intensity time resolved" /> |
− | <caption>Fig. - Time resolved measurement of the YFP fluorescence intensity. Light induction of the PsaD promoter. </caption> | + | <caption>Fig. - Time resolved measurement of the YFP fluorescence intensity. Light induction of the PsaD promoter (white area). It can clearly be seen that at the time of illumination the fluorescence intensity of YFP increases, indicating a light induced expression of the protein. </caption> |
| </div> | | </div> |
| </div> | | </div> |