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− | <a href="#!"><img | + | <a href="#!"><img alt="project summary" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> |
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<h2 style="margin: 0;padding: 10px 0;">Project Summary</h2> | <h2 style="margin: 0;padding: 10px 0;">Project Summary</h2> | ||
− | <p style="margin: 0"> | + | <p style="margin: 0">abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 |
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<div id="sponsor" class="col m3 s12 row"> | <div id="sponsor" class="col m3 s12 row"> | ||
− | <a href="https:// | + | <a href="https://2019.igem.org/Team:Fudan"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0.5em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/7/7f/T--Fudan-TSI--Logo0-crop-grey.png"> |
</a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | ||
</a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点,排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点,排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | ||
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<li><a href="/Team:Fudan-TSI/Description">Background</a></li> | <li><a href="/Team:Fudan-TSI/Description">Background</a></li> | ||
<li><a href="/Team:Fudan-TSI/Design">Design</a></li> | <li><a href="/Team:Fudan-TSI/Design">Design</a></li> | ||
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li> |
− | <li><a href="/Team:Fudan-TSI/Judging"> | + | <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li> |
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− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li> |
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− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li> |
− | <li><a href="/Team:Fudan-TSI/ | + | <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li> |
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+ | <li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li> | ||
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<li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li> | <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li> | ||
<li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li> | <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li> | ||
− | <li><a href="/Team:Fudan-TSI | + | <li><a href="/Team:Fudan-TSI">© 2019</a></li> |
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− | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg | + | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg, 2005 Songhu Rd, Shanghai |
</div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | ||
</div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn |
Revision as of 14:13, 29 August 2019
Project design
Zijie Yang and Dr. Cai, through discussions, came up with the first draft of 3-layer transmembrane logic gates with mutual effort, Ziyan Yu, through literal reading, designed the experiments to optimize the Notch core. Everyone on the team contributed to the improvement of the project.
Experiment
Based on previous experience designing genetic circuits response to different input densities, Zijie Yang and Ziyan Yu came up with the genetic circuits for the project. Dr. Cai gave comments and provided several references aiding the improvement.
Ziyan Yu, Chuyue Zhang, Yuyan Zeng, and Rongrong Du were responsible for the majority of molecular cloning. Fangfei Ye, Mitchell Wan, and Jingbo Fu assisted in some molecular cloning.
Zijie and Ziyan conducted cell culture, transient transfection, and flow cytometry analysis. Zijie constructed the stable cell lines using retroviral infection and cell sorting.
Rongrong purified EGFP protein, attached the protein to beads, which were used in our shaking test for SynNotch activation.
Dr. Cai provided numerous suggestions and advice on experimental design and data analysis.
Mitchell conducted the Addon thought experiments (ribo, TALE, and T2) with advice from Dr. Cai.
InterLab
Mitchell Wan completed the experiments with help from Zijie Yang and people from iGEM team Fudan-CHINA.
Model
Yiheng Wang came up with the model on transcriptional amplifier.
Lingwei Zhang simulated multi-cell interactions and evolution with a population perspective. Lingwei also built our software based on his modeling results.
Zijie Yang and Yijie Pan provided suggestions to different details in our modeling.
Software
Lingwei Zhang wrote up our software.
Integrated HP
Jingbo Fu, Yuyan Zeng, Fangfei Ye, and Rongrong Du made huge efforts in advertising our project and human practice activities to the public.
Jingbo led the surveys, organized the interviews with researchers and doctors. Everyone on the team heartily helped and participated in various HP events.
Zijie Yang and Ziyan Yu invited Prof. Blacklow to Fudan University, and accompany his visit.
Jingbo and Yuyan kept in contact with high schools, organizers responsible for the summer camp, and other undergraduate students. They led our presentations for them, and everyone on the team participated actively.
Jingbo designed and trained high school students from Qibao Dwight High School during this summer, also organized the multi-disciplinary debate on project safety and ethical topics.
Fangfei planned and assembled the Bio-Art Display and everyone on the team took part in making the Display come true.
Art design
Fangfei Ye is responsible for all art design, which includes our team logo, team flag, team uniform (Dr. Cai gave comments), team name card, brochures, our posters, as well as materials related to our human practice events.
Wiki
Tian Huang built the wiki template with Materialize framework. Fangfei Ye and Tian coded the site, while rest of the team worked on content write-up. Dr. Cai gave comments on the coding and writeup.
Support
Dr. Lu and Dr. Cai secured the primary funding for the team. Dr. Cai provided the lab space. All team members participated in the crowdfunding campaign.