Difference between revisions of "Team:Fudan-TSI/Experiments"

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This html document is created by Tian Huang for Team Fudan iGEM 2018.
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We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2.
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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
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<h3>What should this page contain?</h3>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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<h3>Inspiration</h3>
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<ul>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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    <title>2019 Team:Fudan-TSI Experiments</title>
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                        <h1>Experiments</h1>
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                        <span>tba tba</span>
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                    <h1>Experiments</h1>
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                    <p><span>tba tba</span></p>
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                    <img alt="2018 team Fudan title attributions" src="https://static.igem.org/mediawiki/2018/5/5f/T--Fudan--title_attri.jpg">
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                <main style="margin: 0;">
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                    <div class="section container">
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                        <h2>title</h2>
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                        <p>
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                            content content
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                        </p>
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                        <h2>title</h2>
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                        <p>
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                            Fangfei Ye is responsible for all <a href="/Team:Fudan-TSI/Design_Intention" target="_blank">art design</a>, which includes our team logo, team flag, team uniform (Dr. Cai gave comments), team name card, brochures, our posters, as well as materials related to our human practice events.
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                        </p>
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                    </div>
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                    <div class="section container">
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                      <div class="figureHolder">
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                          <p>PCR and subcloning were performed using standard methods. Detailed primer sequences are <a href="/Team:Fudan-TSI/Primers">provided</a>. All constructs were verified by Sanger sequencing.
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                          </p>
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                          <p>Cells were cultured in DMEM supplemented with 10% FBS (HyClone), 100 U/ml penicillin, 100 μg/ml streptomycin and 1x GlutaMax (Gibco). Transient transfections were performed using Lipofectamine 2000 (Invitrogen) and Opti-MEM (Gibco). Viral packaging, infection and fluorescence-activated cell sorting were performed using standard methods.
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                          </p>
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                          <p>Images, unless otherwise indicated, were captured using an inverted epifluorescence microscope (IX-81, Olympus) and a sCMOS camera (pixel size = 0.3222 &mu;m; Zyla 5.5, Andor; 20x objective N.A.  0.75) and were controlled by <a href="http://www.micro-manager.org/" target=_blank>Micro-Manager software</a>.
 +
                          </p>
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                          <p>All statistical analysis was performed using Prism (Graphpad) and <a href="http://rsbweb.nih.gov/ij/developer/macro/macros.html" target=_blank>ImageJ</a>. All experiments were independently performed in triplicates; unless otherwise indicated. Images were combined and annotated in Powerpoint for presentation. Representative images are shown.
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                          </p>
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                      </div>
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                                  <th>Our protocols as PDF files</th><th>&nbsp;</th>
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                                  <th>Download</th>
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                              </tr>
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                              <tr>
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                                  <td>Be a Good Lab Member</td><td><i>GoodLabPractices.pdf</i></td>
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                                  <td><a href="https://2018.igem.org/File:T--Fudan--GoodLabPractices.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
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                              <tr>
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                                  <td>Molecular Cloning</td><td><i>MolecularCloning.pdf</i></td>
 +
                                  <td><a href="https://2018.igem.org/File:T--Fudan--MolecularCloning.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
 +
                              <tr>
 +
                                  <td>Tissue Culture</td><td><i>CellCulture.pdf</i></td>
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                                  <td><a href="https://2018.igem.org/File:T--Fudan--CellCulture.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
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                              <tr>
 +
                                  <td>Make a Stable Cell Line</td><td><i>MakeStableCellLine.pdf</i></td>
 +
                                  <td><a href="https://2018.igem.org/File:T--Fudan--MakeStableCellLine.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
 +
                              <tr>
 +
                                  <td>Cell Sorting</td><td><i>FACS.pdf</i></td>
 +
                                  <td><a href="https://2018.igem.org/File:T--Fudan--FACS.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
 +
                              <tr>
 +
                                  <td>Cell Staining</td><td><i>FixStain.pdf</i></td>
 +
                                  <td><a href="https://2018.igem.org/File:T--Fudan--FixStain.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
 +
                              <tr>
 +
                                  <td>Time-lapse Live-cell Imaging</td><td><i>TimeLapseImaging.pdf</i></td>
 +
                                  <td><a href="https://2018.igem.org/File:T--Fudan--TimeLapseImaging.pdf" target="_blank"><i class="fa fa-download"></i></a></td>
 +
                              </tr>
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                          </table>
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                      </div>
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                      <p style="color:grey">
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                          For practical reasons, all full-length protocols are in Chinese.
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                      </p>
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                  </div>
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                </main>
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            <!--Abstract on content page-->
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            <div id="abstractContent" class="z-depth-2">
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                <a href="#!"><img alt="project summary" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
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                <div class="container">
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                    <h2 style="margin: 0;padding: 10px 0;">Project Summary</h2>
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                    <p style="margin: 0">abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract abstract due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5 due sep 5
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                </a>
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                <a href="#FudanWrapper" class="btn">
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                    <i class="fa fa-angle-up" style="font-size: 48px;line-height: 45px"></i>
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            <!-- Footer with sponsors and contact methods -->
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                        <div id="sponsor" class="col m3 s12 row">
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                            <a href="https://2019.igem.org/Team:Fudan"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0.5em 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2019/7/7f/T--Fudan-TSI--Logo0-crop-grey.png">
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                            </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
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                        </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
 +
                        </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png">
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                        </a>
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                            <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size: 18px">Repeated Evolution in vivo</h3>
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                        </div>
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                        <div id="usefulLinks" class="col m9 s12 row">
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                            <div class="col s12 l6 row">
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                                <div class="col s12 m4">
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                                  <span><a href="/Team:Fudan-TSI/Description">Project</a></span>
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                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
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                                    </ul>
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                                </div>
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                                <div class="col s12 m4">
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                                    <span><a href="/Team:Fudan-TSI/Results">Results</a></span>
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                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
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                                    </ul>
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                                </div>
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                                    <span><a href="/Team:Fudan-TSI/Model">Model</a></span>
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                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
 +
                                    </ul>
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                                </div>
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Revision as of 14:10, 29 August 2019

<script src="https://code.jquery.com/jquery-1.11.3.min.js"></script> 2019 Team:Fudan-TSI Experiments

Experiments

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Experiments

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2018 team Fudan title attributions

title

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Fangfei Ye is responsible for all art design, which includes our team logo, team flag, team uniform (Dr. Cai gave comments), team name card, brochures, our posters, as well as materials related to our human practice events.

PCR and subcloning were performed using standard methods. Detailed primer sequences are provided. All constructs were verified by Sanger sequencing.

Cells were cultured in DMEM supplemented with 10% FBS (HyClone), 100 U/ml penicillin, 100 μg/ml streptomycin and 1x GlutaMax (Gibco). Transient transfections were performed using Lipofectamine 2000 (Invitrogen) and Opti-MEM (Gibco). Viral packaging, infection and fluorescence-activated cell sorting were performed using standard methods.

Images, unless otherwise indicated, were captured using an inverted epifluorescence microscope (IX-81, Olympus) and a sCMOS camera (pixel size = 0.3222 μm; Zyla 5.5, Andor; 20x objective N.A. 0.75) and were controlled by Micro-Manager software.

All statistical analysis was performed using Prism (Graphpad) and ImageJ. All experiments were independently performed in triplicates; unless otherwise indicated. Images were combined and annotated in Powerpoint for presentation. Representative images are shown.

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Our protocols as PDF files  Download
Be a Good Lab MemberGoodLabPractices.pdf
Molecular CloningMolecularCloning.pdf
Tissue CultureCellCulture.pdf
Make a Stable Cell LineMakeStableCellLine.pdf
Cell SortingFACS.pdf
Cell StainingFixStain.pdf
Time-lapse Live-cell ImagingTimeLapseImaging.pdf

For practical reasons, all full-length protocols are in Chinese.

project summary

Project Summary

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