Team:XHD-WS-Wuhan-A/experiment/future work/
Future Work
In furture We will use microfluidic technology to separate gastric cancer cells. In order to facilitate our detection, we separated gastric cancer cells, and achieved the proliferation of gastric cancer cells by immobilized culture technology. Finally, the results were obtained by detecting the fixed cancer cells.
Early gastric cancer tissue is limited to gastric cancer in gastric mucosa and submucosa, and can be divided into uplift type, flat type and concave type according to the macroscopic morphology.Advanced gastric cancer refers to gastric cancer whose infiltration depth of cancer tissue exceeds the submucosal layer, and can be divided into four types: polyp type, localized ulcer type, infiltrating ulcer type and diffuse infiltration type.
Gastric cancer cells are difficult to obtain and cause great pain to patients, so it is most appropriate to use circulating tumor cells for detection.
Therefore, it is of great significance to use microfluidic technique to separate circulating tumor cells and examine them in situ
Due to the different adhesion ability of tumor cells and normal cells to the surface of glass, nano-rough surface can be formed by reactive ion etching (RIE) to successfully capture different types of tumor cells.
Just like this, the blood captures circulating tumor cells through the capture plate, and then changes the conditions of the channel medium, induces the expression of promoters with inducers, and USES microRNA profiles in gastric cancer cells at different stages to achieve the detection of gastric cancer cells at different stages.
As shown in the figure above, first, the patient's blood adheres to the cavity through the cells, and the circulating tumor cells will adhere to the cavity wall, while the blood cells will not. After the cells were obtained, the culture medium was replaced by the liquid, and the proliferation of tumor cells was realized in the attachment cavity.
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XHD-WS-Wuhan-A
Wuhan High School
No.538,Gongxiu Road,Xiaoshan District,
Wuhan,Hubei Province,China