Parts
Mtr Pathway Components
MtrA
![](https://static.igem.org/mediawiki/2019/5/5c/T--Westminster_UK--P-CP-1.png)
Part of a membrane-spanning protein complex (MtrABC) which is necessary for dissimilatory iron reduction through the electron transport pathway of S. oneidensis MR-1. MtrA is required for periplasmic stability of MtrB.
MtrB
![](https://static.igem.org/mediawiki/2019/e/e9/T--Westminster_UK--P-CP-2.png)
Outer membrane component of extracellular iron oxide respiratory system. Required for Fe(III) and Mn (IV) reduction. Supports the composition of MtrC and OmcA.
Sequence and Features
MtrC/OmcA
.![](https://static.igem.org/mediawiki/2019/0/0b/T--Westminster_UK--P-CP-3.png)
An outer membrane cytochrome which plays a critical role in iron reduction through a direct mechanism involving electron transport or in conjunction with MtrC. OmcA is directly involved in the reduction of Fe(III) and Mn (III/Iv) oxides via either direct contact with these metal oxides or other components of the electron transport network. OmcA and MtrC interact directly with each other to form a stable complex that has the capacity to function as part of the electron transport pathway of S. oneidensis MR-1.
BioSurfactant Production Vector
RhlA
![](https://static.igem.org/mediawiki/2019/5/56/T--Westminster_UK--P-CP-4.png)
Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. Rhamnolipid production plays an important role in swarming motility.
Aerobic Regulatory Transcription Factors
EtrA
![](https://static.igem.org/mediawiki/2019/c/cc/T--Westminster_UK--P-CP-5.png)
Electron transport regulator A (EtrA) involved in the iron homeostasis. Regulates anaerobic growth on fumarate, nitrite, Fe3+, TMAO, DMSO, thiosulfate and sulfite, but not on nitrate nor Mn4.
RpoE
![](https://static.igem.org/mediawiki/2019/1/17/T--Westminster_UK--P-CP-6.png)
RNA polymerase, extracytoplasmic transcription factor E found in Shewanella oneidensis MR-1 strain. Involved as an aerobic response transcription factor.
CRP
![](https://static.igem.org/mediawiki/2019/1/1e/T--Westminster_UK--P-CP-7.png)
cAMP receptor protein involved in iron homeostasis. The expression of many anaerobic respiratory genes in MR-1, including those for the reduction of fumarate, dimethyl sulfoxide, and metal oxides, is regulated by cyclic AMP receptor protein (CRP). CRP i coordinately regulates the expression of catabolic and respiratory pathways in MR-1, including d-lactate dehydrogenase and anaerobic terminal reductases. CRP directly binds to the upstream regions of omcA and mtrC in the presence of cAMP, and activates the transcription of these metal-reduction genes.
PET degradation BioBricks
TphP
![](https://static.igem.org/mediawiki/2019/7/75/T--Westminster_UK--P-CP-8.png)
Terephthalate permease, referred as TphC as well, allows the uptake of TPA inside the cells.