Team:Westminster UK/Basic Part
Parts
Mtr Pathway Components
MtrA
Part of a membrane-spanning protein complex (MtrABC) which is necessary for dissimilatory iron reduction through the electron transport pathway of S. oneidensis MR-1. MtrA is required for periplasmic stability of MtrB.
MtrB
Outer membrane component of extracellular iron oxide respiratory system. Required for Fe(III) and Mn (IV) reduction. Supports the composition of MtrC and OmcA.
Sequence and Features
MtrC/OmcA
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An outer membrane cytochrome which plays a critical role in iron reduction through a direct mechanism involving electron transport or in conjunction with MtrC. OmcA is directly involved in the reduction of Fe(III) and Mn (III/Iv) oxides via either direct contact with these metal oxides or other components of the electron transport network. OmcA and MtrC interact directly with each other to form a stable complex that has the capacity to function as part of the electron transport pathway of S. oneidensis MR-1.
BioSurfactant Production Vector
RhlA
Required for rhamnolipid surfactant production. Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. Rhamnolipid production plays an important role in swarming motility.
Aerobic Regulatory Transcription Factors
EtrA
Electron transport regulator A (EtrA) involved in the iron homeostasis. Regulates anaerobic growth on fumarate, nitrite, Fe3+, TMAO, DMSO, thiosulfate and sulfite, but not on nitrate nor Mn4.
RpoE
RNA polymerase, extracytoplasmic transcription factor E found in Shewanella oneidensis MR-1 strain. Involved as an aerobic response transcription factor.
CRP
cAMP receptor protein involved in iron homeostasis. The expression of many anaerobic respiratory genes in MR-1, including those for the reduction of fumarate, dimethyl sulfoxide, and metal oxides, is regulated by cyclic AMP receptor protein (CRP). CRP i coordinately regulates the expression of catabolic and respiratory pathways in MR-1, including d-lactate dehydrogenase and anaerobic terminal reductases. CRP directly binds to the upstream regions of omcA and mtrC in the presence of cAMP, and activates the transcription of these metal-reduction genes.
PET degradation BioBricks
TphP
Terephthalate permease, referred as TphC as well, allows the uptake of TPA inside the cells.
