Team:Vilnius-Lithuania/Parts
Parts
Overview
Until this moment, bacterial optogenetic regulation was only possible at the transcriptional level. However, the existing optogenetic controllers, even with reasonable control, all the transcriptional regulators have an imperfect design.
Vilnius-Lithuania iGEM 2019 team created a collection that consists of parts that allow three-level modular control with light in bacteria.
Modular transcription regulators CarH and Q-Pas1 are chimeric proteins that open up a possibility for researchers to control bacteria function at the transcriptional level with green and near-infrared (NIR) light.
Light-inducible regulation system for the plasmid copy number enables to maintain a low plasmid copy number at the growth stage and increase it for the production stage.
Finally, the post-translational control system works by increasing the POI's degradation rate and thus allows new possibilities to control bacterial dynamics at the post-translational level with rapid kinetics.
Basic Parts
| Part Name | Type | Short Description |
|---|---|---|
| BBa_K3032000 | Tag | Light-inducible protein degradation tag (159AtLOV2) |
| BBa_K3032001 | Tag | Light-inducible protein degradation tag (160AtLOV2) |
| BBa_K3032002 | Tag | Light-inducible protein degradation tag (161AtLOV2) |
| BBa_K3032003 | Tag | Light-inducible protein degradation tag (162AtLOV2) |
| BBa_K3032004 | Tag | Light-inducible protein degradation tag (163AtLOV2) |
| BBa_K3032005 | Tag | Light-inducible protein degradation tag (164AtLOV2) |
| BBa_K3032006 | Tag | Light-inducible protein degradation tag (165AtLOV2) |
| BBa_K3032007 | Tag | Light-inducible protein degradation tag (166AtLOV2) |
| BBa_K3032008 | Tag | Light-inducible protein degradation tag (167AtLOV2) |
| BBa_K3032009 | Tag | Light-inducible protein degradation tag (168AtLOV2) |
| BBa_K3032010 | Tag | Light-inducible protein degradation tag (169AtLOV2) |
| BBa_K3032011 | Tag | Light-inducible protein degradation tag (144AsLOV2) |
| BBa_K3032012 | Tag | Light-inducible protein degradation tag (139AsLOV2) |
| BBa_K3032013 | Coding | CarH repressor |
| BBa_K3032014 | Regulatory | CarH promoter |
| BBa_K3032015 | Coding | ho1 (heme oxygenase 1) |
| BBa_K3032016 | Coding | BphP1 (bacterial phytochrome photoreceptor) |
| BBa_K3032017 | Coding | Gal4_Q-PAS1 repressor |
| BBa_K3032018 | Coding | LexA75_Q-PAS1 repressor |
| BBa_K3032019 | Coding | LexA90_Q-PAS1 repressor |
| BBa_K3032020 | Coding | LexA91_Q-PAS1 repressor |
| BBa_K3032021 | Coding | LexA92_Q-PAS1 repressor |
| BBa_K3032022 | Coding | LexA93_Q-PAS1 repressor |
| BBa_K3032023 | Coding | LexA94_Q-PAS1 repressor |
| BBa_K3032024 | Coding | PpsR2 repressor |
| BBa_K3032025 | Regulatory | consensus LexA promoter |
| BBa_K3032026 | Regulatory | strong LexA promoter |
| BBa_K3032027 | Regulatory | cUAS Gal4 promoter |
| BBa_K3032028 | Regulatory | cUAS 3A Gal4 promoter |
| BBa_K3032029 | Regulatory | ColE based strong LexA promoter |
| BBa_K3032030 | Regulatory | ColE based cUAS Gal4 promoter |
| BBa_K3032031 | Regulatory | T7 based strong LexA promoter |
| BBa_K3032032 | Regulatory | T7 based cUAS Gal4 promoter |
| BBa_K3032033 | Regulatory | ColE based PpsR2 promoter (1) |
| BBa_K3032034 | Regulatory | ColE based PpsR2 promoter (2) |
| BBa_K3032035 | Regulatory | pBR_crtE promoter |
| BBa_K3032036 | Coding | BLUF-cI repressor |
| BBa_K3032110 | Regulatory | EL222 regulated promoter based on Anderson (0.86) |
| BBa_K3032111 | Regulatory | EL222 regulated promoter based on Anderson (0.47) |
| BBa_K3032112 | Regulatory | EL222 regulated promoter based on Anderson (0.16) |
Composite Parts
| Part Name | Type | Short Description |
|---|---|---|
| BBa_K3032050 | Composite | sfGFP tagged with light-inducible protein degradation tag (159AtLOV2, Ready for Expression) |
| BBa_K3032051 | Composite | sfGFP tagged with light-inducible protein degradation tag (160AtLOV2, Ready for Expression) |
| BBa_K3032052 | Composite | sfGFP tagged with light-inducible protein degradation tag (161AtLOV2, Ready for Expression) |
| BBa_K3032053 | Composite | sfGFP tagged with light-inducible protein degradation tag (162AtLOV2, Ready for Expression) |
| BBa_K3032054 | Composite | sfGFP tagged with light-inducible protein degradation tag (163AtLOV2, Ready for Expression) |
| BBa_K3032055 | Composite | sfGFP tagged with light-inducible protein degradation tag (164AtLOV2, Ready for Expression) |
| BBa_K3032056 | Composite | sfGFP tagged with light-inducible protein degradation tag (165AtLOV2, Ready for Expression) |
| BBa_K3032057 | Composite | sfGFP tagged with light-inducible protein degradation tag (166AtLOV2, Ready for Expression) |
| BBa_K3032058 | Composite | sfGFP tagged with light-inducible protein degradation tag (167AtLOV2, Ready for Expression) |
| BBa_K3032059 | Composite | sfGFP tagged with light-inducible protein degradation tag (168AtLOV2, Ready for Expression) |
| BBa_K3032060 | Composite | sfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression) |
| BBa_K3032061 | Composite | sfGFP tagged with light-inducible protein degradation tag (144AsLOV2, Ready for Expression) |
| BBa_K3032062 | Composite | sfGFP tagged with light-inducible protein degradation tag (139AsLOV2, Ready for Expression) |
| BBa_K3032070 | Composite | mRFP1 reporter under light-inducible CarH promoter (Ready for Expression) |
| BBa_K3032071 | Composite | CarH repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032072 | Composite | CarH repressor under medium Anderson (0.33) promoter (Ready for Expression) |
| BBa_K3032073 | Composite | CarH repressor under weak Anderson (0.1) promoter (Ready for Expression) |
| BBa_K3032074 | Device | Ready to use mRFP1-CarH device (strong) |
| BBa_K3032075 | Device | Ready to use mRFP1-CarH device (medium) |
| BBa_K3032076 | Device | Ready to use mRFP1-CarH device (weak) |
| BBa_K3032080 | Composite | ho1 under medium Anderson (0.33) promoter (Ready for Expression) |
| BBa_K3032081 | Composite | BphP1 under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032082 | Composite | Gal4_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032083 | Composite | LexA75_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032084 | Composite | LexA90_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032085 | Composite | LexA91_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032086 | Composite | LexA92_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032087 | Composite | LexA93_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032088 | Composite | LexA94_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032089 | Composite | PpsR2 repressor under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032090 | Composite | sfGFP reporter under light-inducible consensus LexA promoter (Ready for Expression) |
| BBa_K3032091 | Composite | sfGFP reporter under light-inducible strong LexA promoter (Ready for Expression) |
| BBa_K3032092 | Composite | sfGFP reporter under light-inducible cUAS Gal4 promoter (Ready for Expression) |
| BBa_K3032093 | Composite | sfGFP reporter under light-inducible cUAS 3A Gal4 promoter (Ready for Expression) |
| BBa_K3032094 | Composite | sfGFP reporter under light-inducible ColE-LexA promoter (Ready for Expression) |
| BBa_K3032095 | Composite | sfGFP reporter under light-inducible ColE-Gal4 promoter (Ready for Expression) |
| BBa_K3032096 | Composite | sfGFP reporter under light-inducible T7-LexA promoter (Ready for Expression) |
| BBa_K3032097 | Composite | sfGFP reporter under light-inducible T7-Gal4 promoter (Ready for Expression) |
| BBa_K3032098 | Composite | sfGFP reporter under light-inducible ColE-PpsR2 promoter (1) (Ready for Expression) |
| BBa_K3032099 | Composite | sfGFP reporter under light-inducible ColE-PpsR2 promoter (2) (Ready for Expression) |
| BBa_K3032100 | Composite | sfGFP reporter under light-inducible pBR_crtE promoter (Ready for Expression) |
| BBa_K3032113 | Composite | EL222 protein under strong Anderson (1) promoter (Ready for Expression) |
| BBa_K3032114 | Composite | EL222 protein under medium Anderson (0.47) promoter (Ready for Expression) |
| BBa_K3032115 | Composite | EL222 protein under weak Anderson (0.16) promoter (Ready for Expression) |
| BBa_K3032116 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.16) |
| BBa_K3032117 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.24) |
| BBa_K3032118 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.33) |
| BBa_K3032119 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.16) |
| BBa_K3032120 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.24) |
| BBa_K3032121 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33) |
| BBa_K3032122 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.16) |
| BBa_K3032123 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.24) |
| BBa_K3032124 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.33) |
| BBa_K3032125 | Composite | Light-regulated plasmid copy number device (0.16) |
| BBa_K3032126 | Composite | Light-regulated plasmid copy number device (0.47) |
| BBa_K3032127 | Composite | Light-regulated plasmid copy number device (0.86) |
| BBa_K3032128 | Intermediate | Light-regulated plasmid copy number device intermediate (0.86) |
| BBa_K3032129 | Intermediate | Light-regulated plasmid copy number device intermediate (0.47) |
| BBa_K3032130 | Intermediate | Light-regulated plasmid copy number device intermediate (0.16) |
Part Collection
| Part Name | Type | Short Description |
|---|---|---|
| BBa_K3032010 | Tag | Light-inducible protein degradation tag (169AtLOV2) |
| BBa_K3032060 | Composite | sfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression) |
| BBa_K3032013 | Coding | CarH repressor |
| BBa_K3032014 | Regulatory | CarH promoter |
| BBa_K3032128 | Intermediate | Light-regulated plasmid copy number device intermediate (0.86) |
| BBa_K3032129 | Intermediate | Light-regulated plasmid copy number device intermediate (0.47) |
| BBa_K3032130 | Intermediate | Light-regulated plasmid copy number device intermediate (0.16) |
| BBa_K3032116 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.16) |
| BBa_K3032117 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.24) |
| BBa_K3032118 | Composite | Light-regulated plasmid copy number device (0.16) with mRFP1 (0.33) |
| BBa_K3032119 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.16) |
| BBa_K3032120 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.24) |
| BBa_K3032121 | Composite | Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33) |
| BBa_K3032122 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.16) |
| BBa_K3032123 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.24) |
| BBa_K3032124 | Composite | Light-regulated plasmid copy number device (0.86) with mRFP1 (0.33) |
| BBa_K3032074 | Device | Ready to use mRFP1-CarH device (strong) |
| BBa_K3032076 | Device | Ready to use mRFP1-CarH device (weak) |