Team:Victoria Wellington/Protocols

Protocols

Restriction Digest

The process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases

Gibson assembly

A molecular cloning method which allows for the joining of multiple DNA fragments in a single, isothermal reaction

Transformation

The process by which foreign DNA is introduced into a cell

Competent cells

E. coli cells are more likely to incorporate foreign DNA if their cell walls are altered so that DNA can pass through more easily

Expression

Express the gene of interest.

Purification

Purification of proteins