Team:Victoria Wellington/Collaborations
Team
Victoria
Collaborations
Opentrons OT2 Robot Collaboration
We collaborated with the UC Davis Team who also received an Opentrons OT2 Robot. The goal of the collaboration was to collect data to determine the accuracy of the OT2 robot relative to pipetting by hand. This involved carrying out three sets of serial dilutions both by hand and with the OT2 robot. There were a total of six teams involved in this data analysis. We anticipated that the OT2 pipetting would be more accurate overall that pipetting by hand, as there is less chance of experimental error.
Overall results
Particle Data:
iGEM Team:
1
2
3
4
5
6
Combined
r^2
OT2
0.7188
0.9518
0.9898
0.9980
0.9956
0.9207
0.8152
Team
Member
0.9470
0.9570
0.9953
0.9982
0.9895
0.8220
0.9027
Multiple
r^2
values determined through linear regression model, reported with p-values < 2.2e-16.
Fluorescein Data:
iGEM Team:
1
2
3
4
5
6
7
Combined
r^2
OT2
0.9818
0.9411
0.9977
0.9943
0.9956
0.9207
0.9804
0.9435
Team
Member
0.9526
0.9939
0.9940
0.9981
0.9895
0.8220
0.9828
0.9034
Multiple
r^2
values determined through linear regression model, reported with p-values < 2.2e-16.
What this means
The overall results showed that the OT2 overall performed better with the Particle Data Protocol, however, performed worse with the Fluorescein Data Protocol. This poor performance looks to be a result of one of the teams' data skewing the combined R
If the unusual value from Team 1 were to be omitted we would find that the Opentrons OT2 robot does indeed outperform the human pipetting by hand. If this experiment were to be carried out again in future it would be optimal if we could obtain a larger sample size.
We also have some reservations regarding the repeated values between protocols for Teams 5 and 6. Although it is possible they managed to obtain these results, it seems unlikely. Next time it would also be advisable to ensure everyone involved carried out the protocols as instructed, perhaps by double-checking with the team if the data looked unusual.
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