Safe Project Design
To obtain the pyocyanin, we were able to rely on our secondary project advisor, Dr. Mark Pellegrino and his biology lab team.
In order to comply with iGEM Safety Standards, we made sure that pseudomonas aeruginosa was not directly handled by anyone on our iGEM team since it is not on the White List. They were able to obtain pyocyanin from pseudomonas aeruginosa by purifying it using chloroform.
We have made certain decisions about the design to reduce risks including:
choosing a non-pathogenic chassis
choosing parts that will not harm humans, animals, or plants
following the safety protocols for iGEM and our university at all times
