Our general project is based on a degradation pathway found in Pseudomonas pseudomallei 22, a pathogenic bacterium naturally found in the soil. Two genes, glpA and glpB, were identified as increasing tolerance to glyphosate and enabling the breakage of the C-N bond, resulting in the formation of AMPA. We plan to clone these genes into E. coli to enable it to degrade glyphosate. In E. coli AMPA can be further broken down into phosphorus and methylamine by a native C-P lyase. Through this, we hope to create E. coli that can break down glyphosate in different environments such as water.