Team:UIUC Illinois/Experiments

UIUC iGEM Notebook and Experiments

HOME TEAM PROJECTS PARTS SAFETY HUMAN PRACTICES JUDGING FORMS

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Weekly Lab Notebook

Ally

Jenny

Pauline

Pauline

Pauline

Ally

Jenny

Pauline

Pauline

Pauline

Ally

Jenny

Pauline

Pauline

Pauline

Protocols

Listed below are the protocols we used in our lab to clone glyphosate resistant genes into E. Coli. Remember to also check out the Safety page to how to perform these protocols safely.

Preparing and Running an Agarose Gel

Qiagen Spin Miniprep

Restriction Digestion

Restriction Digest and Ligation

Gibson Reaction

IPTG Induction Experiments

E. Coli Competent Cells

Overnight Growth of Colony

Competent Cell Efficiency Kit

Transformation of Competent Cells

Transformation of Cells

LB Broth and SOB Media

Growth Curve Protocol

Fluorescence Calibration Curve

Silica Bead Particle Absorbance Standard Curve

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