Apple Lee - full-time summer team member, wetlab work for FASyn Operon, characterized BBa_J61100, helped write wiki content. Marti Gendel - full-time summer team member, wetlab work for ParB and FASyn Operon, collaboration and human practices point of contact, helped write wiki content. Nathan Sattah - full-time summer team member, wetlab work for Exporter Operon, helped write wiki content. Jacob Wolf - full-time summer team member, wetlab work for Alk Operon, coded model, coded wiki, helped write wiki content. Cian Colgan - Copresident of GeneHackers, project idea, planning, summer-team organization and oversight. Rachael Filzen - Copresident of GeneHackers, summer-team organization and oversight.
GeneHackers members completed all of the wetlab work, research designs, and project documentation with supervision, feedback and advice from Professor Ben Glick (our PI) and Professor Laurens Mets; Michael Disare, Simone Rauch, Haneul Yoo, DJ Speed, William Grubbe, and Jessy Morgan(our graduate student advisors); Cian Colgan and Rachael Filzen (Co-Presidents of GeneHackers, assisted the summer team); GeneHackers members also built our wiki page and assembled our Jamboree poster and presentation with assistance from those mentioned above. Nathan Tague, PhD candidate Boston University who works in biofuel synthesis, provided integrated human practices advice including a warning that continuous production could lead to genetic drift because our production scheme has a disadvantage. Professor Laurens Mets also provdided design advice suggesting that the buildup of alkanes would lead to increased toxicity and that perhaps an exporting channel would solve this issue. Furthermore, Professor Mets suggested that in order to overcome the relative inefficiency of photosynthesis, we could optimize the Fatty acid synthesis pathway which led to the creation of our mathematical model and the FASyn Operon. Chris Kuffner, PhD candidate Boston University, provided advice on wiki design, experimental design, project feedback, general suggestions and navigation of the iGEM tournament. We used laboratory protocols developed previous GeneHackers teams as well as by iGEM, the Drummond laboratory and the Glick laboratory. The University of Chicago Office of Research Safety provided us with online biosafety training. DNA sequencing was performed for us by UChicago’s Comprehensive Cancer Center DNA Sequencing Facility. DNA was synthesized for us by IDT and Twist Biosciences. Much thanks to the Glick lab, Drummond Lab and the Dickinson Lab for allowing us to use their laboratory space and equipment during the summer. Generous stipend funding was provided by the Biological Sciences Collegiate Division of the University of Chicago.