Team:UCL/Experiments
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Cloning
Proteins
Bacterial Culturing
Mammalian Techniques
Protocols
Here are pdfs of the experimental protocols and methods we used in the lab over the summer.
Cloning
Agarose Gel Electrophoresis
Bacterial Heat Transformation
Calcium Chloride Solution for Making Competent Cells
Golden Gate Assembly
Hot Start Taq DNA polymerase Colony PCR
DNA Ligation
Miniprep DNA Plasmid Isolation
Q5 High Fidelity PCR
PCR Clean-up
Restriction Digestion
Proteins
Cell Free Protein Synthesis
Dynamic Light Scattering (DLS)
FLAG-Tag Purification
Preparation of Cleared Lysates – Sonication
SDS PAGE
Small-Scale Protein Expression from T7 Promoters in BL21(DE3) Cells
Strep-Tag Purification
Western Blot (for Strep-Tagged Proteins)
Quantification of fluorescent protein loading into proteinaceous nanocompartments
Bacterial Cell Culturing
Antibiotic Preparation
LB Broth Preparation
Scale-Up Culture Overnight Cultures/label>
Mammalian Techniques
Annexin V-FITC Assay Protocol for Annexin V-FITC Apoptosis Staining/Detection Kit
Mammalian Cell Passaging/Subculturing
Cell Counting
