Improved part
BBa_K2946003– Inhibitory CAR
We chose to improve an ITIM (immune tyrosine-based inhibitory motif) which is an intracellular domain of KIR3DL1. Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer (NK) cells and subsets of T cells that differs by their inner and extra cellular sub-domains.
Long cytoplasmic chain transmits inhibitory signals via ITIM while short ones transmit activation signals via TYRO protein (tyrosine kinase binding protein).
The expression of KIR on natural killer cell membrane has a major effect on their regulation. Therefore, a well expressed KIR outcome as a better controlled NK cell activity. By replacing the scFv attached to the original PART with an α-HLA scFv and using an RNA-based expression system we ensure higher expression rate of the KIR protein hence, NK and T cells native activities are meticulous and precise. the FACS results the expression of our improved part was higher than UCSF 2010 similar part as you can see in the following graph:
**further information and elaborated results can be seen in BBa_K2946003