Team:Stony Brook/Notebook
Notebook
Here are the experiments that we worked on for each week. Click on each week's header for more details.
Preparatory Work
- Received seeds to start growing plants
- Practiced growing plants
- Planned for lab work in the summer
Week 1 (6/23-6/29)
- Started streaking plates of bacteria
- Troubleshooted gel electrophoresis
Week 2 (6/30-7/6)
- Got materials for TMV infection and agroinfiltration
- Miniprepped bacteria for backbone
- Started PCR of construct
Week 3 (7/7-7/13)
- Started infecting leaves with TMV
- Continued PCR and miniprepping of
parts - Started using HiFi assembly to
create plasmid
Week 4 (7/14-7/20)
- Began work for characterization
- Troubleshooted HiFi plasmid
- Troubleshooted PreLim plasmid
Week 5 (7/21-7/27)
- Continued with characterization experiments
- Made competent cells
- Continued inoculating leaves
with TMV
Week 6 (7/28-8/3)
- Sequenced HiFi plasmid
- Continued with characterization experiments
- Began prep for agroinfiltration
Week 7 (8/4-8/10)
- Began agroinfiltration
- Began work to improve a previous
biobrick - Religated plasmids for
characterization
Week 8 (8/11-8/17)
- Continued agroinfiltration
- Started imaging plants
Week 9 (8/18-8/24)
- Continued plating bacteria
- Continued imaging plants
Fall Work (8/25-10/1)
- Finishing up experiments from the summer in the fall
- Finishing up imaging plants
- Finishing up characterization experiments
Preparatory Work
- Received seeds to start growing plants
- Practiced growing plants
- Planned for lab work in the summer
Week 2
6/30 - 7/6
- Got materials for TMV infection and agroinfiltration
- Miniprepped bacteria for backbone
- Started PCR of construct
Week 3
7/7 - 7/13
- Started infecting leaves with TMV
- Continued PCR and miniprepping of parts
- Started using HiFi assembly to create plasmid
Week 4
7/14 - 7/20
- Began work for characterization
- Troubleshooted HiFi plasmid
- Troubleshooted PreLim plasmid
Week 5
7/21 - 7/27
- Continued with characterization experiments
- Made competent cells
- Continued inoculating leaves with TMV
Week 6
7/28 - 8/3
- Sequenced HiFi plasmid
- Continued with characterization experiments
- Began prep for agroinfiltration
Week 7
8/4 - 8/10
- Began agroinfiltration
- Began work to improve a previous biobrick
- Religated plasmids for characterization
Fall Work
8/25 - 10/1
- Finishing up experiments from the summer in the fall
- Finishing up imaging plants
- Finishing up characterization experiments
