Team:SZTA Szeged HU/Measurement

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Measurement

Our project’s main aim is to provide a bioindication-based device, which is able to detect the production of microcystin, the flagship-toxin during algal blooming. The bacteria containing our Biobricks, are able to forecast the transcription of those genes that play a role in the production of microcystins. The tentative bidirectional promoter of the above mentioned gene cluster was used to produce GFP in three different ways, the amount of which was measured by its fluorescence. The hypothesis is that cyanobacteria (Microcystis aeruginosa) produce cytoplasmic or exported activators of the bidirectional promoter when blooming. We grow up algal cultures under non-blooming (control) and blooming conditions for ten days. We measured the OD600/OD750 of these cultures and took out samples of them each day. The effect of the medium and the lysated algal cells on GFP production was investigated. The fluorescence data were graphed as a fuction of the population density.