Team:SYSU-Medicine/Parts
<!DOCTYPE html>
PARTS
Experience
Our team has validated some characterization of existing parts.
| Part number | Name | Type | Despription |
| BBa_K1806005 | T7+ ibPB RNA Thermometer + RFP | Composite | Regulateing the expression of RFP through temperature and ITPG |
| BBa_I712004 | CMV promoter | regulatory | starting the expression of gene in eukaryon |
| BBa_I714891 | EGFP | coding | Encoding the protein eGFP |
Basic parts
Our team has built these basic parts, and you can click on the part number to see the details.
| Part number | Name | Type | Despription |
| BBa_K2942701 | Riboswitches | rbs | Regulateing transcription, translation, splicing and the stability of RNA |
| BBa_K2942704 | Carboxypeptidase G2 (CPG2) | coding | Encoding the protein CPG2 with flag tag |
| BBa_K2942703 | Nitroreductase | coding | Encoding the protein nitroreductase with flag tag |
Composite Parts
We combined the basic part including promoter and protein coding sequences, which are presented in the following table. You can click on the part number to see the details.
| Part number | Name | Type | Despription |
| BBa_K2942705 | CMV promoter+eGFP | Composite | starting the expression of eGFP in eukaryon |
| BBa_K2942706 | CMV promoter+ Riboswitches+eGFP | Composite | Starting and regulating the expression of eGFP in eukaryon |
| BBa_K2942708 | CMV promoter+ Riboswitches+ CPG2+eGFP | Composite | Starting and regulating the expression of CPG2 in eukaryon |
| BBa_K2942707 | CMV promoter+ Riboswitches+Nitroreductase+eGFP | Composite | Starting and regulating the expression of Nitroreductase in eukaryon |
Improved Parts
Our team have improved nitroreductase sequences by Codon humanization and add a flag on it. The improved parts (in blue) and original existing parts (in red) are presented below. You can click on the part number to see the details.
| Part number | Name | Type | Despription |
| BBa_K917004 BBa_K2942707 |
Nitroreductase | coding | human codon optimization on the original sequence and flag added |