<!DOCTYPE html> Attributions

ATTRIBUTIONS

Overview

During this time, we accomplished a lot of work, including but not limited to: design of our project, wet lab experiments, modelling, human practice, wiki, parts, art design and presentation. The work is mainly done by the following team members, which is shown in this table.

	Wet Lab	Modelling	Human Practice	Wiki	Parts	Art Design	Presentation
Zibin Huang		√	√				√
Xiaohang Liang		√	√	√			√
Xingyu Li	√		√	√		√	√
Xiangxue Li			√	√		√
Lizhi Guan	√		√	√
Yuantao Liu	√		√	√		√
Shijian Song	√		√	√	√	√	√
Fengmin Yang	√			√	√
Weisheng Lu		√		√
Jiayi Zeng	√		√	√		√
Shanshan Chen	√		√	√		√
Yihui Wang	√			√	√
Shunjie Hu	√		√	√	√
Xi Wang	√		√	√

Attributions in detail

Zibin Huang

-Modelling: mainly responsible for modelling and finished the modeling part of our work.

-Human Practice: participated in the 6th CCiC in Shenzhen and helped modify the modeling part of the public account, as well as discussing the current social reaction of the new therapy represented by the oncolytic virus therapy.

Xiaohang Liang

-Modeling: model establishment, parameter estimation, data analysis, paper writing.

-Human Practice: attend 2019 CCiC, scientific article (When Synthetic Biology meet with Mathematics) writing.

-Wiki: wiki writing(modeling).

-Presentation: modeling part.

Xingyu Li

-Wet lab: cell culture, molecular cloning, plasmid extraction, flow cytometry, virus harvest, reverse transcription and identification.

-Human Practice: interview with professors, questionnaire design and analysis, writing for official accounts.

-Art Design: homepage design and advise.

-Wiki: outline for project description, manuscript for homepage, background and problem definition.

-Presentation: introduction.

Xiangxue Li

-Art Design: In charge of art design including logo, bookmark, name cards as well as our poster and presentation slides.

-Wiki: designing, painting illustrations and making animation.

-Human practice: making stickers.

Lizhi Guan

-Wet lab: cell culture (293T, HCT-116, HCT-8, Bxpc, A549, B16 tumor cell lines etc. ), measurement of the infection rate and lethality effect of M1 virus on tumor cells. Molecular cloning like extraction of plasmids, DNA purification and so on. Pre-experiment of directed evolution that was mentioned in the initial ideas, including serial passaging of M1 virus.

-Human Practice: participated in one of the interviews with professors as well as wrote an article in our official accounts.

-Wiki: future perspectives, inspiration and initial ideas.

Yuantao Liu

- Wet lab: construction of M1 virus. Molecular cloning, plasmid extraction, PCR, reverse transcription and in vitro transcription

-Wiki: assistant person in charge of webpage production including website visual design, coding, testing.

-Human Practice: interview with professors

-Art Design: give some suggestions to the web page

Shijian Song

-Wet lab: construction of M1 virus. Molecular cloning such as DNA purification, DNA gel extraction, extraction of plasmids, PCR, RTPCR and so on.

-Human practice: managing our Wechat Official Account, participated in all of the interviews with professors and the dinner party with local virologists.

-Wiki: the writing of the Collaboration part.

-Parts: Molecular cloning such as extraction of plasmids.

-Art Design: The dubbing actor of our team video.

-Presentation: sharing the lab results part.

Fengmin Yang

-Wet Lab: General manager of the experimental group. Conducting molecular cloning experiments including PCR, restriction enzyme digestion of plasmid and PCR product, gel extraction purification and construction of recombinant plasmids. Cell culture, flow cytometry and cell viability analysis (Bxpc-3, Bt474, J82, A549, Hct116, Hct8, Mcf-7, HepG2, etc.). Pre-experiment of directed evolution of M1 virus on several cell lines (Bt474, A549, J82, Bxpc-3). Data analysis.

-Parts: gel extraction purification.

-Wiki: results.

Weisheng Lu

-Modeling: data visualization.

-Wiki: main person in charge of webpage production including website visual design, coding, testing.

Jiayi Zeng

-Wet lab: cell culture (Vero, BHK-21, 293T), measurement of the titer of M1 virus on tumor cells. Amplification of M1 virus used for cells and animal experiment. Measurement of the infection rate of M1 virus on tumor cells. Pre-experiment of directed evolution that was mentioned in the initial ideas, including serial passaging of M1 virus.

-Human Practice: Participated in several interviews with professors. Wrote the first article in our official accounts. Participated in the visit to GCP office in SYSU Cancer Center and had consultation with the director. Had the supporting education in Meizhou city. Participated in the public survey, making and spreading the questionnaire.

-Wiki: Human Practice, Education and Public Engagement.

-Art Design: The dubbing actor of our team video

Shanshan Chen

Student leader of our team.

-Wet lab: digestion, gel extraction purification, construction of recombinant plasmids, PCR, in vitro transcription, transformation, virus harvest, virus purification.

-Human Practice: interview with professors

-Wiki: writing

-Art Design: giving advice

Yihui Wang

-Wet lab: cell culture (cell thawing/subculture/cryopreservation),as well as testing M1 virus infection rate in different cells with different treatments via flow cytometry.

-Parts: building and characterizing the basic and composite parts, also characterizing some existing parts.

-Human practice: interviewing with the professor.

-Wiki: writing for the safety and parts chapter.

Shunjie Hu

-Wet lab: mainly involved in molecular experiments, including the construction of the synthetic virus. Offering assistance to teammates who carried out cell experiments is also included.

-Human Practices: conducting interviews with several professors with teammates as well as recording and collating the contents of those interviews. Following the support team to the local high school to promote the knowledge of synthetic biology and responsible for the writing of some of the contents of the education.

-Parts: construction of components.

-Wiki: assisting in writing the introduction of parts.

Xi Wang

-Wet lab: cell culture (cell thawing/subculture/cryopreservation), operating the cell viability assays (MTT assay) and testing M1 virus infection rate in different cells with different treatments via flow cytometry.

-Human practice: heading for MeiZhou to give lessons about synthetic biology to students in Jiaying middle school and introduction of iGEM and the project we have been working in. Writing an article about the relationship between food and synthetic biology and published on our team’s official account.

-Wiki: results part.

Aknowledgement

Prof. Guangmei Yan

Primary PI of SYSU-Medicine team. Professor Yan made constructive suggestions on our project, provided us with all the resources needed to implement the project, and allowed us to complete all the experiments in his lab.

Prof. Yuan Lin

Secondary PI of our team. Professor Lin gave guidance on all aspects of our project, including wet experiments, human practice, etc. He evaluated all our whimsical ideas and guided us to implement them bit by bit.

Zhiqian Zhang

Instructor of our team. He followed up the whole process of our project, from the initial recruitment to the pre-competition preparation, and provided us with a lot of valuable guidance.

Guangen Wu

Instructor of our team. He mainly guided us to finish the wet experiment, and his meticulous and patient attitude gave us a lot of help.

PhD Li Guo

Advisor of our team. She gave us many useful suggestions about the nature of oncolytic virus M1.

Longyuan Zhou

Advisor of our team. He inspired us in the design of our experiment.

Xiaojun Su

Advisor of our team. He made helpful suggestions for our experiment.

Ziqing Lin

He gave us advice and guidance on purifying and concentrating the virus.

Xiaozhi Yang

He gave us advice and help for the experiments.

Youyu Wu

She advised us on experiments and provided reagents.

Deli Song

He gave us advice and help for the experiments.

Xingwen Su

He assisted us in ordering reagent materials.

Liumei Wu

She provided reagents for us.

Jiayu Zhang

She provided us with the space to do experiment.

Prof. Penghui Zhou

We consulted with him about the prospect of tumor immunology and oncolytic virus therapy which inspired us a lot.

Prof. Xinwen Chen, Prof. Musheng Zeng, Prof. Ren Sun, Prof. Yerong Su, Prof. Pei Xu, Prof. Miao Xu and Prof. Qian Zhong

The virologists shared their own research experiences and inspirations at the dinner talk, and discussed with our team the current situation and prospects of oncolytic virus.

PhD Zhenhua Huang, Yang Liu, Jia Dan, Bingzheng Lu, Jingyi Tan and other researchers in Prof. Guangmei Yan’s Laborotary

They provided advice and help for our experiment.

Prof. Yanhui Guan

The advisor Yanhui Guan suggested us to use Possion distribution for the mutation, for the convenience of calculation.

Prof. Na You

Our biostatistics teacher Na You in the school of mathematics suggested us to test the relationship of mutation, and enhance us to find the functional form of mutation for every site.

Prof. Jia Li

Our math experiment teacher Jia Li in the school of mathematics guided us to try to use hsv for image intensification.

Junyu Lu

Junyu Lu from the College of Life Science told us the probable value of virus death rate.

Haiyang Zhang

He assisted us to improve the structure of ODE model, and guide us to do t-test based on data, which really help us a lot. We really love him.

Dong

Dong from South China Statistical Science Research Center have guide us in cancer data analysis.

Kailiang Liu

Kailiang Liu from the Likelihood Lab suggested us to solve the problem of out of memory on the server.

Funding

Professor Guangmei Yan's laboratory
Guangzhou Virotech Pharmaceutical Technology CO., LTD
National Students' platform for innovation and entrepreneurship training program

Team:SYSU-Medicine/Attributions