Team:SNU India/Design


CLONING STRATEGY

Composite Part Assembly

Step 1

Digestion of RBS + TAL

Figure 1
Figure 1
Schematic representation of double digestions of RBS+TAL
Step 2

Linearisation of RFP in pSB1C3

Figure 2
Figure 1
Schematic representation of linearisation of RFP part
Step 3

Ligation of linearised product and insert

Figure 3
Figure 1
Schematic representation of ligation product of RFP+RBS+TAL
Step 4

Digestion of ligated product with Xba-I and Pst-I

Figure 4
Figure 1
Double digestions of RPF+RBS+TAL
Step 5

Linearisation of T7+RBS part using Spe-I and Pst-I

Figure 5
Figure 1
Linearisation of T7+RBS part
Step 6

Ligation of linearised product obtained in Step-5 and digested product in Step-4

Figure 6
Figure 1
Schematic representation of final Construct

Sub-Cloning into pSB3C5 vector

Step 1

Double Digestion of part from pSB1C3 with Xba-I and Pst-I

Figure 7
Figure 1
Double Digestion of composite part in pSB1C3 with Xba-I and Pst-I
Step 2

Ligation of digested product and Xba-I and Pst-I digested plasmid backbone of pSB3C5

Figure 8
Figure 1
Schematic representation of composite part in pSB3C5