Team:Queens Canada/Experiments

Overview

Laboratory experiments were conducted in tandem with the hardware and software design. The progression can be summed up as follows:
1) Designing a one-step membrane assay for THC detection, using lipophilic membranes and a commercial anti-THC antibody
2) Modeling of single chain variable fragments (ScFv) and antigen binding fragments (Fab), to link them to fluorescent proteins
3) Using the models to design a library of ScFv and Fab, linked to fluorescent proteins
4) Cloning into pET vectors for high protein expression
5) Testing proteins for THC binding ability on novel one-step membrane assay
6) Testing antibody binding limit using one-step membrane assay

Links to Protocols

Novel Immunoassay for lipophilic antigen detection

Diagnostic Restriction Digest

gBlock Gibson Assembly

Glycerol Stocks

His-Tag Nickel Chromatography

LB Broth and Agar Plates

Liquid Culture

Lysis by Sonication

MiniPrep

Resuspension of gBlocks Gene Fragment

Streaking Plates