Team:Purdue/Safety

Safe Project Design
     RICE was designed using only BSL 1 chasses Escherichia coli and Pseudomonas fluoresence using parts not harmful to humans/animals/plants. Although the idea of testing the functionality of the NodC in live p.fluorescens is convenient for the purpose of screening transformed colonies in the intended chassis, we decided to keep the vector in the pGEM backbone.
Safe Lab Work
     Prior to conducting any laboratory work, all Purdue iGEM team members underwent several training procedures in accordance with Purdue’s Radiological and Environmental Management (REM)* policies and requirements.
Biological Safety Training
     The topics covered included a discussion about the National Institute of Health Biohazard Risk Groups, personal protective equipment, proper handling of waste and biohazard spills, and security measures.
Laboratory Safety Awareness Training
     We learned how to identify chemical hazards, read safety data sheets and chemical labels, minimize personal hazards, use safety equipment, and understand emergency response procedures and individual safety responsibilities.
Bloodborne Pathogen Training
     We discussed what bloodborne pathogens are, how they are transmitted, and how to prevent and respond to exposure.
Standard Operating Procedures
    We reviewed the procedures for storing and handling specific chemicals including the different cells. Additionally, we reviewed the protocols and procedures for the growth and storage of Escherichia coli st. DH5α and BL21 along with Pseudomonas fluorescens st. F113.
     Foot Notes and References
  1. *REM governs the safety of biological labs at Purdue University (1)
  2. “Purdue University – Radiological And Environmental Management”. Purdue.edu N.p., 2019. Web 30 August 2018.