Team:Pasteur Paris/Medals
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Bronze medal criteria
We have added quantitative experimental characterization data to existing parts from the Registry of Standard Biological Parts, as expected. The part BBa_K1073022 (Lien vers Registry) corresponds to the constitutively expressed chromoprotein eforRed designed by iGEM Team Braunschweig in 2013. We expressed the chromoprotein eforRed in Escherichia coli and characterised it in two ways: a flow cytometry analysis and by fluorescence microscopy.
| Explanation | Criteria achieved |
| Registration and Giant Jamboree Attendance | We registered for the iGEM Giant Jamboree and we can't wait to be there ! |
| Competition Deliverables | We met the deadlines for every required deliverable : in particular the Wiki and the Judging Form. We will exhibit our poster during the Giant Jamboree, and present our project DIANE on Saturday, November 2nd |
| Attributions | We completed the Attributions page on our wiki |
| Project Inspiration and Description | We completed the Project Description page on our wiki. We explained how and why we chose to work on our iGEM project : DIANE, a new bacterial diagnostic device. |
| Characterization | We have added quantitative experimental characterization data to existing parts from the Registry of Standard Biological Parts, as expected. The part BBa_K1073022 corresponds to the constitutively expressed chromoprotein eforRed designed by iGEM Team Braunschweig in 2013. We expressed the chromoprotein eforRed in Escherichia coli and characterised it in two ways: a standard growth curve of protein concentration vs OD458 was recorded and a flow cytometry study was led. The part BBa_K1033923 corresponds to the spisPink, a pink chromoprotein designed by iGEM13_Uppsala in 2013. We expressed the chromoprotein spisPink in Escherichia coli and characterised it with a standard growth curve of protein concentration vs OD458 . |
Silver medal criteria
| Explanation | Criteria achieved |
| Validated Part | We have created a new BioBrick, as expected : BBa_K3176020 (lien Registry). This part corresponds to a DNA aptamer selected against E. faecium strains specifically. After ten rounds of SELEX (Systematic Evolution of Ligands by EXponential enrichment), we obtained its sequence. We succeeded in coating carbon nanotube electrodes with our aptamer. We characterized it by studying its binding affinity and its specificity to E. faecium, through electrochemical measurements. |
| Collaboration | This year, in collaboration with three Parisian iGEM teams, we have organized the InParis European Meetup, during which 19 european teams came to Paris for presentations, workshops and poster sessions in a Jamboree-like state of mind. We have also collaborated with the Aptamer Hub to provide help and tips to the next iGEM teams that wish to use aptamers like we have. We answered several social media challenges and answered surveys from other teams. Finally, we collaborated with iGEM IISER Bhopal, in India: after exchanging our views on antibiotic resistance, they were able to spread our survey to city and hospital Indian doctors. You’ll find all the details here. (lien vers Collaborations) |
| Human Practices | During the conception of DIANE, we took advice from many professionals in very different fields to create a device best adapted to the need for fast diagnosis. Our jurists have worked with the Ethical Committee of Pasteur to try and obtain patentability for our machine. Because the issue of antibiotic resistance goes far beyond the creation of DIANE, it was important for us to raise awareness to a young public, as well as to non-scientists: you can read all about it here (lien vers la page Human Practice) |
Gold Medal criteria
| Explanation | Criteria achieved |
| Integrated Human Practices | We have interviewed several field doctors and specialists so that the conception of the device would meet the very demanding criteria of such an environment. |
| Demonstrate | We demonstrated a complete approach of product design incorporating a synthetic biology solution to a rapid bacterial identification. We succeeded in obtaining specific sequences of aptamers for E. faecium after a SELEX selection, and modeling their folding. We bound aptamers to carbon nanotube electrodes and detected a signal in the presence of bacteria. We proved that this signal was specific to the bacterial strain. We performed this demonstration in a millifluidic chip, designed and laser-etched out of PMMA, to get our own proof of concept. In order to develop a new diagnostic device suitable for users, we led numerous research and interviews. We created it as a waterproof and robust (to avoid false results) device, lightweight, small (easily transportable) and easy to use. We designed the whole millifluidic path inside the device and we chose, in collaboration with industrial automation experts, the different components to be added to the system to ensure its automatic function. We finally printed a 1:1 scale model with a 3D printer and photosensitive SLA resin. Learn more about what we demonstrated on this page : |
| Human Practices | During the conception of DIANE, we took advice from many professionals in very different fields to create a device best adapted to the need for fast diagnosis. Our jurists have worked with the Ethical Committee of Pasteur to try and obtain patentability for our machine. Because the issue of antibiotic resistance goes far beyond the creation of DIANE, it was important for us to raise awareness to a young public, as well as to non-scientists: you can read all about it here (lien vers la page Human Practice). |