Team:Pasteur Paris

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    Picto Sciences



    Pasteur Paris 2019


    Nowadays, worldwide, infectious diseases account for 40% of the total 50 million annual estimated deaths. 700,000 of these deaths are due to infections caused by resistant bacteria, with up to 25,000 in Europe alone [1]. Concerning sepsis, someone dies every 5 seconds in the world.



    Sepsis occurs when the immune system develops a very strong response against a bacterial infection, causing widespread (systemic) inflammation throughout the entire body that can lead to tissue damage, organ failure, and death. When sepsis occurs, the body undergoes a cascade of changes, including the formation of blood clots and greater permeability of blood vessels that impede blood flow to the organs. Blood pressure decreases, several organs may be insufficient, the heart is affected, and death may result.


    In the United States, there are approximately 750,000 sepsis cases per year and 75,000 cases in Canada. Mortality varies between 18% and 30% depending on the study, or 215,000 deaths per year, while it is in the order of 40% to 70% in patients with septic shock[1]. In France, there are about 70,000 cases of sepsis per year and nearly 30,000 deaths, Inserm reported in 2015. Mortality in people with sepsis is 27%, but mortality in the most severe form (septic shock) can reach 50%. Sepsis causes as many deaths as myocardial infarction[2].


    One minute.
    A single minute could truly make a difference in the management of patients with sepsis in the Emergency Department (ED). The management of the patients affected by an infection is impacted by a time factor. In that way, diagnostic tests could help to identify high-risk patients, isolate patients as needed, and rapidly initiate treatment. In the most serious cases, particularly in sepsis cases, the need for a rapid and accurate diagnosis then becomes absolute to provide first aid : each second being crucial.

    1 MN


    In such a context, the urgency of the treatment and its vital nature may lead to the prescription of non-targeted treatments. This practice contributes to antibiotic resistance. Antimicrobial resistance is a major concern as the excessive use of antimicrobial drugs is not only making the microorganisms resistant, but also causing severe infections, which are becoming harder to treat.


    No new antibiotics with a new mechanism of action have been developed for 20 years

    158,000 multi-resistant bacterial infections in France

    12,500 deaths are linked to multi-resistant bacterial infection in France

    Between 71 and 441 million euros of overconsumption of antibiotics in France

    If providing quick and safe treatment in the short term is necessary, providing the right treatment is life-saving in the long term is better

    Then we should act on both.

    How ?

    DIANEDiagnosis is Now Easier

    Our Solution

    Our project aims to create a portable, precise and rapid device for diagnosis, in order to detect pathogens through the use of an aptamer-based sensing electrode. Current diagnostic methods often require bacterial culture, implying at least 24 hours of incubation, which is not ideal for time sensitive clinical cases. In this context, improving the speed, sensitivity, and specificity of bacterial detection is crucial.

    Given the emergency of the antibiotic resistance issues, research is dense and diverse in the field of infectious diseases diagnosis. Existing and future techniques can be focused on pathogen identification (ID) and antibiotic susceptibility testing (AST). Today, a lot of methods are still culture based. They are manual and highly time-consuming since the first bacterial identification results are revealed in at least 24 hours. Molecular based techniques, using DNA amplification by PCR, require knowledge of the primers in advance. Mass spectrometry methods and flow cytometry are certainly faster since they allow a diagnostic in about 10 hours, but not cheaper than the previous methods. When we look at the techniques in development and the future techniques, we can observe that they are more and more sophisticated. For example, imaging-based methods will use microscopy to scan a population of bacterial cells and to analyze a multitude of parameters to discriminate resistance from susceptible strains. On another side, microfluidics techniques will allow the isolation of each cell to measure its metabolically activity in the presence of antibiotics. Other futuristic methods involve next generation sequencing, or e-noses to detect volatile compounds as an electronic aroma signature to identify bacteria. These latest technologies, in the research or development phase use state-of-the-art technologies and require a large package of scientific expertise. They often allow the analysis of a large mass of samples and they reduce the diagnostic time to few hours. But they are expensive and applied in machines that are imposing in size and that require expertise and material to manage. Therefore, they cannot be transportable on more "uneven" terrain, in humanitarian missions for example.

    For more information about our project click here!


    Integrating product design into our project has helped us create a real, working, simple and efficient solution for customers to use in bedside medicine and in humanitarian context. Using the methodology of product design, by performing user research, sketches, model making and prototyping, we have assessed the different needs and expectations of potential users. Working with a professional from industrial design has helped us achieve our main goal : design a working product from scratch, using interviews and experiments to get answers on hypotheses we have made on our way to our final device. Working with a professional business in medical devices automation has allowed us to step up our device and fully automate the use of DIANE using pumps and valves. Finally, designing a scenario for our users has helped us understand all the challenges users might face during their daily work, allowing us to try and solve those problems to facilitate the use of DIANE.

    For more information about the design of the device click here!


    DIANE was led by a team of ten students from different Parisian schools and universities. Among them, Sup’Biotech (biology), ESPCI Paris (physics and chemistry), AgroParisTech (engineering), ENS Cachan (chemistry), Chimie ParisTech (chemistry), and Faculté Jean Monnet (intellectual property law). The team was accompanied by a project director, Dr. Deshmukh Gopaul (Director of Research at the Institut Pasteur) and three coaches (PhD students at the Institut Pasteur). For more information about our team click here!

    For more information about our team click here!

    References :

    [1] INSERM, “Résistance aux antibiotiques”, INSERM, online since the 22/03/18, available on :, visited the 11/04/19

    [2] Martin GS, Mannino DM, Eaton S et al. The Epidemiology of Sepsis in the United States from 1979 through. NEJM 2003 ; 348 (16): 1546-1554; O’Brien JM, Ali NA, Aberegg SK, Abraham Edward. Sepsis. The American Journal of Medicine. 2007; 120 (12): 1012-1022.