Team:Newcastle/Parts
Parts Overview
New Parts
For our project, we developed a range of basic and composite parts that could be used for the detection of biomarkers associated with Parkinson's disease. New basic parts were used with other basic parts found on the registry to build a host of new composite parts. The new basic and composite can be found below:
| Name | Type | Description | Design | Build | Test |
|---|---|---|---|---|---|
| BBa_K3206000 | Composite | Glycine biosensor | ✓ | ✓ | ✓ |
| BBa_K3206001 | Basic | gcvBp promoter | ✓ | ✓ | ✓ |
| BBa_K3206002 | Basic | ChaC CDS | ✓ | - | - |
| BBa_K3206003 | Basic | PepD CDS | ✓ | ✓ | - |
| BBa_K3206004 | Basic | GcvA CDS | ✓ | ✓ | - |
| BBa_K3206005 | Basic | GcvR CDS | ✓ | ✓ | - |
| BBa_K3206006 | Composite | ChaC constitutive expression | ✓ | - | - |
| BBa_K3206007 | Composite | PepD constitutive expression | ✓ | ✓ | - |
| BBa_K3206008 | Composite | GcvA constitutive expression | ✓ | ✓ | ✓ |
| BBa_K3206009 | Composite | GcvR constitutive expression | ✓ | ✓ | ✓ |
| BBa_K3206010 | Composite | 1-eicosanol biosensor | ✓ | ✓ | ✓ |
| BBa_K3206011 | Basic | adhEp2 promoter | ✓ | ✓ | ✓ |
| BBa_K3206012 | Basic | His-tagged eGFP | ✓ | ✓ | ✓ |
| BBa_K3206013 | Basic | pET RBS | ✓ | ✓ | ✓ |
| BBa_K3206014 | Composite | His-tagged eGFP under Bba_J23100 Promoter | ✓ | ✓ | ✓ |
| BBa_K3206015 | Composite | PepD gene under BBa_I1051 promoter | ✓ | - | - |
Used Parts
A variety of basic parts found on the iGEM Registry of Standard Biological Parts were used in conjunction with new parts submitted into the registry to create new composite parts in the form of transcriptional units. The used parts can be found below:
| Name | Type | Description | Design | Build | Test |
|---|---|---|---|---|---|
| Bba_J23100 | Basic | Constitutive promoter | - | ✓ | ✓ |
| BBa_B0034 | Basic | Constitutive ribosome binding site | - | ✓ | ✓ |
| Bba_E1010 | Basic | Highly engineered red fluorescent protein from Discosoma striata coral | - | ✓ | ✓ |
| Bba_B0015 | Basic | Terminator | - | ✓ | ✓ |
| Bba_J04450 | Composite | mCherry Fluorescent Protein | - | ✓ | ✓ |
| Bba_K515105 | Composite | sfGFP with J231000 Promoter | - | ✓ | ✓ |
| Bba_K1033907 | Composite | fwYellow Chromoprotein | - | ✓ | ✓ |
| Bba_K592011 | Composite | cjBlue Chromoprotein | - | ✓ | ✓ |
| Bba_I1051 | Basic | Lux cassette right promoter | - | - | - |
