Team:NYMU-Taipei/Basic Part
Basic Parts
Part: BBa_K3268000
mOR103-15
This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004). We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
- The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
- Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.
