The main goal of our project, E.Phoenix, was to develop a brand new biosystem that could detect mutagens surrounding us. We hoped to mutate genes by exposing E. coli to mutagenic surroundings and detected the mutation by toxin gene.
We summited five toxic genes as basic parts and also their composite parts with lac promoter and RBS. What’s more, we provided composite parts with Quorum Sensing system containing red fluorescent protein. We conduct a functional test to quorum sensing system. All BioBricks applied in our project are sequence-confirmed and the following table shows all of them:
