![](https://static.igem.org/mediawiki/2019/6/6f/T--Manchester--Medals--Bronze1.png) |
Registration and Giant Jamboree Attendance |
![](https://static.igem.org/mediawiki/2019/7/75/T--Manchester--Medals--Humans.png) |
![](https://static.igem.org/mediawiki/2019/7/7a/T--Manchester--Medals--Bronze2.png) |
Competition deliverables |
![](https://static.igem.org/mediawiki/2019/e/e4/T--Manchester--Medals--Check.png) |
![](https://static.igem.org/mediawiki/2019/3/31/T--Manchester--Medals--Bronze3.png) |
Attributions |
Attributions |
- Clearly distinguished between student’s work and what we got help with.
- Mentioned every person that has helped us along the way
- Noted training offered
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![](https://static.igem.org/mediawiki/2019/7/7a/T--Manchester--Medals--B4.png) |
Project Inspiration and Description |
Project Description |
- Clearly showed a concise description of Cutiful
- Detailed explanation of our inspiration and why it is important
- Appropriate references
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![](https://static.igem.org/mediawiki/2019/7/7b/T--Manchester--Medals--B5.png) |
Characterisation |
Contribution |
- Characterised 3 parts: BBa_J23102, BBa_J23106, and BBa_K199118 by measuring RFU and normalising it with Optical Density at 600 and 660 nm.
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![](https://static.igem.org/mediawiki/2019/e/eb/T--Manchester--Medals--S1.png) |
Validated Part |
Fragrance
Colours
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- Expression and characterisation of codon optimised and His-Tagged TAL, C3H and COMT enzymes,the first three enzymes in the vanillin biosynthesis pathway.
- Prove of their functionality by production of p-coumaric acid, caffeic acid and ferulic acid, the first three intermediates in the vanillin pathway, detected and quantified using UPLC-MS/MS.
- Characterised mRFP1 containing a C-terminal secretion signal (HylA) and proved it through SDS-PAGE and qualitative data.
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![](https://static.igem.org/mediawiki/2019/5/50/T--Manchester--Medals--S2.png) |
Collaboration |
Collaboration |
- Collaborated with six different projects, providing expertise in website coding, modelling, and sharing results.
- Gained a broader, more accessible audience through our Edinburgh overgrad collaboration of our questionnaire.
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![](https://static.igem.org/mediawiki/2019/4/4a/T--Manchester--Medals--S3.png) |
Human Practices |
Human Practices |
- Attended the community science festival at Manchester museum where we interacted with the public sharing ideas and gaining feedback
- Contacted several stakeholders which gave us industrial insight into our product
- Discussed the science behind our project with scientists at the synthetic biology symposium in Manchester.
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![](https://static.igem.org/mediawiki/2019/1/18/T--Manchester--Medals--G1.png) |
Integrated Human Practices |
Human Practices |
- Integrated feedback from the public by modelling a kill switch.
- Integrated feedback from stakeholders by testing our bacteria in different Salt and Shampoo conditions
- Integrated feedback from the scientists at MIB by implementing the fragrance branch of Cutiful
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![](https://static.igem.org/mediawiki/2019/3/36/T--Manchester--Medals--G2.png) |
Improve a Previous Part |
Human Practices
Colour
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- Achieved improved expression of sfGFP by changing the promoter from T7 (BBa_I746909) to pTet (BBa_K2906000). This allows sfGFP to be more tightly controlled and to express higher levels of visible fluorescence, especially in the first few hours.
- Transferred BBa_I746909 iGEM provided construct into a different backbone in order to provide a fair quantitative comparison.
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![](https://static.igem.org/mediawiki/2019/2/25/T--Manchester--Medals--G3.png) |
Model your project |
Modelling
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- Chromoproteins chosen based on modelling of molar extinction coefficients
- Fragrance molecule vanillin chosen based on modelling of a static isochoric sphere as the headspace, concentrations of various odorants were calculated at detection level. Vanillin held the lowest concentrations of all.
- tested fragrances.
- Implemented a previous model to test for allergenicity of our compounds
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![](https://static.igem.org/mediawiki/2019/3/31/T--Manchester--Medals--G4.png) |
Demonstrate your work |
Demonstration
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- We demonstrated that E. coli DH5⍺ and BL21(DE3) attach to hair
- We proved that our system works through coating natural hair samples with our coloured bacteria
- We proved that bacteria do not die under realistic conditions: swimming in chlorinated or saline water, or with the use of shampoo
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