Colony PCR	DNA Purification from Enzymatic Reactions
LB Media	PCR Amplification
PCR Product Purification	Plasmid Miniprep
Chemically Competent Cells	Protein Overexpression
Restriction Digest	Transformation
Ethanol Precipitation	RNase Assay
RNase Inhibitor Test	His-tag Purification of Cas13a
Size Exclusion Chromatography Purification of Cas13a	Cas13a Activity Assay
In vivo Fluorescence Assay	RNA Mango Purification
In vitro transcription	Flourescent assay

Team:Lethbridge HS/Experiments

PROTOCOLS