The working laboratory began, and the tasks were scheduled.
Laboratory
In order to verify the protein-protein interaction between our two target proteins, we used laser energy polarization method. We fused the donor protein CFG and the receptor protein YFG with the two target proteins respectively, and determined whether the two proteins interacted by measuring the loss of fluorescence intensity of CFP. After replacing the medium with fluorescent imaging medium, the imaging begins about 2 hours, and then the image is acquired every 1 hour. We observed the change of SW1990 cells from blue to yellow under 440 nanometer excitation light, which demonstrated the interaction between our two target proteins.
Human Practice
The Human Practice Committee has been delineated, brainstorming has been carried out and the best ideas have been collected so that they can be realized smoothly in the near future.
June 2019
Abstract
Wiki page planning started and we contacted the graphic designer for help. We also contacted professors of oncology to gain insights into the project. The hardware and mathematic model are preliminarily designed.
Laboratory
In order to verify the role of ALKBH5 in migration and creeping of pancreatic cancer cells, scratch experiments were carried out to verify the effect. We altered ALKBH5 by siRNA knockdown and over-expression plasmid (wild-type/mutant) and then examined the migration and creeping ability of pancreatic cancer cells. The migration and crawling ability of siALKBH5 group was stronger than that of Ctrl group (blank control group), P < 0.01. The migration and crawling ability of ALKBH5-WT group was weaker than that of Ctrl group (blank control group), P < 0.01, which indicated that ALKBH5 inhibited the migration and crawling of pancreatic cancer cells. For the accuracy of the experimental results, we conducted Transwell test. AlKBH5 was also altered by siRNA knockdown and over-expression plasmid (wild-type/mutant) to detect the invasion and migration ability of pancreatic cancer cells. The experimental results were the same as scratch test.
Human Practice
Team leader organizes and plans, tasks have been distributed.
July 2019
Abstract
Poster design and Wiki page content are under way. The final determination of mathematical model and hardware.
Laboratory
ALKBH5 plays an inhibitory role in Wnt/beta catenin signaling pathway. In order to know how ALKBH5 inhibits the Wnt/beta catenin signaling pathway, four important proteins (beta-catenin, p-GSK-3beta, GSK-3beta, beta-tubulin) and ALKBH5 were Western blotted. ALKBH5 was used as independent variable, high expression and low expression were set as independent variables, and four important proteins were used as dependent variables to observe the changes of their respective expression levels. Our results showed that up-regulation of ALKBH5 expression in SW1990 pancreatic cancer cells significantly decreased the expression of beta-catenin, p-GSK-3 beta protein in Wnt/beta-catenin signaling pathway, and the total expression of GSK-3 beta protein remained unchanged. These results indicate that up-regulation of ALKBH5 can enhance the activity of GSK-3 beta from to promote the phosphorylation of beta-catenin, leading to the phosphorylation of beta-c-catenin in pancreatic cancer cells. Atenin decreased and inhibited Wnt/beta catenin signaling pathway.
Human Practice
Team members publicize projects in schools, governments, drug institutions and hospitals respectively, and popularize knowledge about synthetic drugs to the masses.
August 2019
Abstract
Wiki pages are constantly improving. The team participated in the 6th China International Genetically Engineered Machinery Competition (iGEM) Team Exchange Meeting CCiC, and communicated with the participating teams.
Laboratory
In order to understand the induction effect of Hif-1, we used CopGFP to test it. The experimental results show that the fluorescence intensity of CopGFP increases with the increase of CoCl (II) concentration. At 500 uM concentration, the fluorescence intensity decreased significantly. This is the result of high concentration of CoCl (II) inhibiting cell activity. Similar effects were observed in SW1990 cells.
September 2019
Abstract
The Wiki page has been preliminarily completed.
Laboratory
In order to demonstrate that Casp3 can cause death of pancreatic cancer cells by randomly cutting nucleosomes under hypoxia, we conducted a hypoxia-sensing system experiment.
October 2019
Abstract
All plans have been completed.
Laboratory
In order to observe this phenomenon, we use fluorescence microscopy for microscopic examination because different cells can produce different degrees of apoptosis under different conditions.
Lanzhou University
Academic Affair Office of LZU
School of Life Science , LZU
The First Hospital of LZU
Contact Us
E-mail：1746921258@qq.com 752786236@qq.com
Address：No. 222, Tianshui South Road, chengguan district, Lanzhou City, Gansu Province,730000, P. R. China.
