Team:Hong Kong UCCKE/Notebook
Notebook
Week 0 (8/10 - 1/11)
Briefing and Division of Work on the events of the upcoming year,
we are briefed the project criteria and guideline from IGEM.
Lecture by senior form students on scientific knowledge,
e.g. What is ribosome? How to use pipette? How to grow bacteria?
Week 1 (12/11-18/11)
Original members of the team Brainstorm on the theme of IGEM Project by exploring current events of Hong Kong, and concerned about the severe food waste problem.
Week 4-7 (3/12-30/12)
After Discussion, we chose our project focus: Food Waste Problem. We Discussed the goal of the Project, studied the cause and the situation of the problem, brainstormed various ways to resolve it, and came out with an outline.
Week 8-9 (31/12-13/1)
Open Day preparation
Week 19 (18/3-24/3)
Discussed a Complete Conception for the Project
Including the rough structure and features of the product
Assigned Attributions and Groups (experiment, prototype, parts design group)
Week 20 (25/3-31/3)
We listed the events to be done and finalize the project progress and deadlines.
1st experiment drafting
Design the 1st prototype
Week 21 (1/4-7/4)
1st Experiment according to protocolot
Week 22-24 (8/4-28/4)
1st Experiment Result Taking
Paper reading about food waste, understanding the problem
Week 25 (29/4-5/5)
2nd and 3rd experiment drafting
Design the 2nd prototype
Week 26-27 (6/5-19/5)
We discussed on the page allocation and division of Work on the Project Powerpoint
2nd and 3rd Experiment according to Protocol
Week 28 (20/5-26/5)
1st Project Presentation in front of students
Week 29 (27/5-2/6)
Prototype Team meeting on improving 2nd Prototype
We discussed the Division of Work on Wiki Page Content
Week 30 (3/6-9/6)
Senior Form Student’s Lecture on Part Design
2nd and 3rd experiment final result taking
Week 33 (24/6-30/6)
1st experiment final result taking
Week 34 (1/7-7/7)
Coding Class for Wiki
Visit to 香港有機資源教育中心 (Hong Kong Organic Resources Education Centre) and Peninsula Life
Week 36 (15/7-21/7)
4th Experiment Drafting + Data analysis
5th experiment drafting + recent experiment data analysis
Testing and Recording the function of 2nd Prototype
Design the 3rd Prototype
We discussed about the further Project focus and Work Planning in summer
Week 37 (22/7-28/7)
4th Experiment according to Protocol
Recent experiment data analysis
Design the 3rd Prototype
Progress check and modification on 3rd Prototype
Week 38 (29/7-3/8)
3rd Prototype in progress
We start of by having the 3rd Project Presentation. We then did a group reflection on the parts to be improved, then discussed a new and detailed Project PowerPoint guideline. Senior students also gave us a lesson on powerpoint making skills. We then did separate group works.
Wet lab practice, including pipette skills training, spread plate training, Sterile Space training.
Senior form students also taught us the whole process of Genetic Modifying, Central Dogma, and taught us Biotech techniques.
Week 39 (4/8-10/8)
We join the Igem camp organised by Team:Hong_Kong_JSS to practice Biotech lab techniques and exchange opinions on the projects with other schools.
Week 40 (11/8-17/8)
5th Experiment according to protocol
4th Experiment final result taking
Progress check, comparing the past prototypes to their original idea to check effectiveness, Finishing the 3rd Prototype
Week 41 (18/8-24/8)
Preparation for 28/8 presentation
Experiment and prototype team did progress report to each other, then we went through the 28/8 Project presentation work distribution together.
Data analysis
Design the 4th Prototype
Week 42 (25/8-31/8)
Data analysis
We had the 4th Project Presentation. We then did a group reflection with senior students on the parts to be improved.
Week 43 (1/9-6/9)
We have a briefing from Mr. Shek on the improvement of wiki and Powerpoint Presentation, then we went to work on wiki with the given guidelines.
After a brief meeting on evaluating the working progress of each individual, we continue to work on the Wiki Pages according to work distribution, to include graphics, photos and details of our project. We end will a small peer evaluation to check on each other.
5th experiment result taking.
Week 44 (7/9-13/9)
We have the 5th Project Presentation in front of Mr. Shek. Mr. Shek and the Alumni gave us specific suggestions on our presentation skills and content to be focused. We then set the deadline to improve the presentation and do it again next Monday 16/9.
5th experiment final result taking
Meeting on data analysis
With some help from Team:Hong_Kong_UST:
Clone and transform Alpha amylase
Verification of alpha amylase by RE digestion
Week 45 (14/9-20/9)
We again did evaluation and advice-taking on the project presentation. Then we did a progress checking on several events, including hoodies design, wiki design, and collaboration with other iGEM teams to make sure everything is going well. The Human Practice team then gave a report on the recent visit and interview with Eco-greeny. Referring to the advice and inspirations given, we discussed on ways to improve our project, especially exchanging views from the Prototype and Experiment Team.
7th Experiment Drafting according to advice from Eco-greenery.
7th Experiment according to Protocol
With some help from Team:Hong_Kong_UST:
Transform lip8
Colony PCR of lip8
Week 46 (21/9-28/9)
Group meeting to check on progress
Worked on Judging Form
Participated in the HK secondary school iGEM symposium, had our 7th and 8th Project Presentation
With some help from Team:Hong_Kong_UST:
Iodine assay for Alpha amylase (characterisation)
Week 47 (29/9-5/10)
Paper reading and discussion on parts design
Design on 5th Prototype
With some help from Team:Hong_Kong_UST:
Assay 1 and Assay 2 of lip8
Week 48 (6/10-13/10)
We did a progress update and created a schedule for the final few weeks before flying.
Experiment 7 final result taking
Finalize design for 5th prototype
Week 49 (13/10-20/10)
Progress update, senior students show us how to complete the Characterization and Parts Registry section.
Assay 1 according to protocol and result taking.
With some help from Team:Hong_Kong_UST:
Transformation of LipIAF5-2
Wiki Freeze... (21/10)