*1% amylase solution (+ control) added on the top, clone of BBa_K23006 in the bottom
Graph 1: The diameter of clear zone of α-amylase and 1% amylase
The assays lasted for 3 days, results for the iodine test is collected every day.We took the results from 5 α-amylase clone and 1% Amylase. In 3 days, a growth in clear zone and halos around the bacteria with malS clone is observed under iodine solution , which showed malS positive in the degradation of starch. From the graph above, 1% amylase showed a greater starting starch degradation amount with a longer clear zone diameter.Yet, it has a constant diameter of clear zone which seemingly levels off in day 3, and suggests that the degradation rate slows down with time. Compared to 1% amylase , the clones of α-amylase showed a steeper increase in the diameter of clear zone from , thus we can conclude that the effect of α-amylase on starch is proportional to the time taken.
α-amylase is preferred in our project as it continuously produces α-amylase to degrade starch. Following the trend in the graph, it can degrade more starch in the long run. As the process of anaerobic digestion lasts for a long period, continuous increase in degrading effect fits better to our aim.