Team:Hong Kong LFC PC/Collaborations

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PLKLFC_PC

Collaboration



Collaborated with Hong_Kong-CUHK to Host a Meet-up

On 21st September 2019, our team collaborated with Hong_Kong-CUHKto co-organize a regional symposium within Pearl River Delta for ll Hong Kong’s university, Macau University and secondary IGEM teams in CUHK. We provided a splendid platform for all teams to present their ideas and shared each other’s opinions. Through raising questions to other teams, every team, including us, had found our project’s limitations and rooms for improvement. Besides presentation, we had sharing sessions, which were divided to wetlab, drylab and human practice forums. We discussed and brainstormed more constructive ways to improve for other teams. In short, this event had been successfully held and all of us have learnt what to improve in our project.



Collaborated with Greatbay_SZ to Host a Meet-up

We have host a meet up with GreatBay_SZ in Shenzhen. A feasibility study on how to clone a gene in probiotic bacteria was evaluated. GreatBay_SZ also provide us the Nissle 1917 E. coli at October as the host cell for our targeted gene such as uricase and HIU hydrolase.



Collaborated with Hong_Kong_JSS to Troubleshoot Our Projects

On 28th September, we have attend a meaningful conference with all Hong Kong’s secondary school teams. After our first meeting at CUHK, we noticed that each team had done lots of improvements. However, we also discovered and realized more aspects we didn’t conduct well, such as integrated human practice . Moreover, we had suggested some limitations (vessel value) of JSS Team’s project which was investigating the use of copper 2+ ion. Furthermore, we have provided competent cell DH5α, which was stored at -80 °C, as they do not have the -80 °C refrigerator, which resulted in the problem of inefficient replication of the competent cells. We have troubleshot the problems of such inefficiency and offered them our refrigerator for replication of competent cells. In return, JSS Team had given us the Gibson Assembly kit and taught us how to use it for combining two long fragments together, in order to complete a new recombinant plasmid with gene of uricase.