To help make the project a reality, our team has designed several new as well as composite parts to be used in our assays.
Below is a full list of our parts:
Part number | Category | Title |
BBa_K3017001 | RNA | CRISPRi sgRNA for gfp DNA binding - transcription template |
BBa_K3017002 | RNA | CRISPRi sgRNA for rfp DNA binding - transcription template |
BBa_K3017003 | RNA | CRISPRi antisense RNA for derepressing gfp - transcription template |
BBa_K3017004 | RNA | CRISPRi antisense RNA for derepressing rfp - transcription template |
BBa_K3017005 | Terminator | T0 Terminator |
BBa_K3017011 | Coding sequence | dCas9-6XHis |
BBa_K3017060 | Composite part | CRISPRi antisense RNA for derepressing gfp - transcription template with terminator |
BBa_K3017061 | Composite part | CRISPRi sgRNA for gfp DNA binding - transcription template with terminator |
BBa_K3017062 | Composite part | CRISPRi sgRNA for rfp DNA binding - transcription template with terminator |
BBa_K3017064 | Composite part | CRISPRi antisense RNA for derepressing rfp - transcription template with terminator |
BBa_K3017065 | Composite part | Construct for testing CRISPRi sgRNA suppression on fluorescence protein production |
BBa_K3017066 | Composite part | Construct for testing CRISPRi sgRNA de-suppression of CRISPRi sgRNA |
BBa_K3017067 | Composite part | Construct for testing CRISPRi sgRNA cross-talk with non-target DNA |
BBa_K3017068 | Composite part | Construct for testing CRISPRi asRNA cross-talk with non-target DNA |
BBa_K3017069 | Composite part | dCas9-6XHis (medium promoter, medium RBS and double terminator) |
BBa_K3017070 | Composite part | Combined CRISPRi and antisense RNA Toggle Switch - Model circuit |
BBa_K3017081 | Composite part | rfp with 2 strong promoters |
BBa_K3017082 | Composite part | rfp with 3 strong promoters |