Team:HUBU-WUHAN/Collaborations
Collaborations
CAU_China
In Conference of China iGEMer Community (CCiC) we knew that their project is similar to ours. Two projects all involved in the cellulose degradation pathway, and both had the same problem that the enzyme activities were very low or even cannot be detected. To solve this problem, we kept in touch and exchanged our references and protocols to find solutions. They also provided suggestions on experimental design.
ECUST_China
We knew their project in the CCiC guide book which is quite helpful for ours. So, we would like to find help about enzyme activity detection and extracellular protein secretion system hoping they could help us detect secretion efficiency of extracellular proteins by using SDS-PAGE and Western Blot because they were good at it.
Figure 1. SDS-PAGE of intracellular and extracellular proteins in CelA-PhoD, CelA-SP, Cel9D-PhoD.
Figure 2. Western Blot of intracellular and extracellular proteins in CelA-PhoD, CelA-SP, Cel9D-PhoD.
We had already detected some EG enzyme (1,4-β-D-glucan glucanohydrolase) activities by DNS at that time which CtCel9D has the highest enzyme activity. They told us that they would like to know the differences about the enzyme activity (CtCel9D) in different chassis strains to explore the influence. So, we sent our bacterium and shared our enzyme activities’ data to them to show the differences. Besides, we exchanged our protocols.
XMU-China
We first met them in front of their poster in CCiC. We heard their wonderful introduction and were interested in their design. We knew that they want to explore the relationships among species and their project involved in expressing cellulase in cells, thinking that may be helpful to our cellulase expression. So, we had a talk in the meeting place about cellulase. They provided us lots of wonderful suggestions.
Later, we faced problems of the cellulase activity determination and contacted them for help. We knew that they had already expressed cellulase. However, their CBH enzyme (1,4-β-D-glucan cellobilhydrolase) had some problems which cannot work effectively. In order to provide more choices for them, we provided two types of CBH enzyme (1,4-β-D-glucan cellobilhydrolase). In return, they provided their bacterium for us making it a positive control to see if there are some problems of our detecting enzyme activity processes. Their bacterium also supplemented our lack of BGL enzyme (β-1,4- glucosidase).