Method-Protocol
- Characterization
- Cloning Protocol E.coli—Transformation
- Cytotoxicity of Yeast to Ni2+
- Fabrication Method of Ni2+
- Molecular Cloning Plasmid extraction DNA electrophoresis DNA agarose gel—extraction
- Molecular Cloning Protocol DNA—Recombination
- Standard Curves of OD600 and Cell Dry—Weight
- Yeast Cell—Immobilization
- Yeast Transformation—Protocol
- Seamless Cloning
2019.5
We synthesized:
BBa_K3126001 (promoter TEF1)
BBa_K3126002 (promoter GPM1)
BBa_K3126003 (promoter TDH3)
BBa_K3126004 (promoter TEF2)
BBa_K3126005 (hexa-His gene)
BBa_K3126006 (nixA gene)
BBa_K3126007 (TgMTP1t2 gene)
BBa_K3126008 (kanR gene)
BBa_K3126010 (terminator CYC1)
BBa_K3126011 (terminator TDH1)
BBa_K3126012 (terminator PGK1)
BBa_K3126013 (terminator ENO2)
pTEF1-tCYC1
GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCG
pGPM1-tTDH1
GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCAG
pTDH3-tPGK1
GAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCAG
pTEF2-tENO2
GAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCAG
BBa_K3126005(hexa-His)
GGTCTCAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGAGACC
BBa_K3126006(nixA)
GGTCTCACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGAGAGACC
BBa_K3126007(TgMTP1t2)
GGTCTCAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGAGAGACC
BBa_K3126008(kanR)
GGTCTCACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTAGAGACC
2019.6.1 ~ 6.10
Construction plasmids:
pSB1C3-pTEF1/tCYC1
The sequence of pSB1C3-pTEF1/tCYC1
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-pGPM1/tTDH1
The sequence of pSB1C3-pGPM1/tTDH1
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-pTDH3/tPGK1
The sequence of pSB1C3-pTDH3/tPGK1
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-pTEF2/tENO2
The sequence of pSB1C3-pTEF2/tENO2
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
We insert promoter TEF1 and terminator CYC1, promoter GPM1 and terminator TDH1, promoter TDH3 and terminator PGK1, promoter TEF2 and terminator ENO2 into vector pSB1C3 using restriction endonuclease and T4 DNA ligase, respectively.
The pSB1C3 digestion system we used:
pSB1C3 | 15 μL |
EcoRI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 24 μL |
The enzyme system we use:
pSB1C3 | 2 μL |
fragment | 6 μL |
T4 DNA Ligase | 0.5 μL |
T4 Buffer | 1 μL |
ddH2O | 0.5 μL |
Verified pictures of pSB1C3-pTEF1/tCYC1 digested by EcoR1 and Pst1:
Verified pictures of pSB1C3-pGPM1/tTDH1 digested by EcoR1 and Pst1:
Verified pictures of pSB1C3-pTDH3/tPGK1 digested by EcoR1 and Pst1:
Verified pictures of pSB1C3-pTEF2/tENO2 digested by EcoR1 and Pst1:
2019.6.10 ~ 6.20
Construction plasmids:
pSB1C3-BBa_K3126014 (pSB1C3-pTEF1/ hexa-His/ tCYC1)
The sequence of pSB1C3-BBa_K3126014
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126015(pSB1C3-pGPM1/nixA/tTDH1/)
The sequence of pSB1C3-BBa_K3126015
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126016(pSB1C3-pTDH3/TgMTP1t2/tPGK1)
The sequence of pSB1C3-BBa_K3126016
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126017 (pSB1C3-pTEF2/kanR/tENO2)
The sequence of pSB1C3-BBa_K3126017
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
Insertion of gene into plasmid carrying promoter and terminator.
pSB1C3-BBa_K3126014 (pSB1C3-pTEF1/ hexa His/ tCYC1):
Enzyme digestion system:
pSB1C3-pTEF1/tCYC1 | 20 μL |
BamHI | 3 μL |
buffer | 5 μL |
ddH2O | 22 μL |
Seamless cloning system:
pSB1C3-pTEF1/tCYC1 | 2 μL |
hexa-His | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K3126015 (pSB1C3-pGPM1/ nixA/tTDH1):
Enzyme digestion system:
pSB1C3-pGPM1/tTDH1 | 20 μL |
BamHI | 3 μL |
buffer | 5 μL |
ddH2O | 22 μL |
Seamless cloning system:
pSB1C3-pGPM1/tTDH1 | 2 μL |
nixA | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K3126016 (pSB1C3-pTDH3/TgMTP1t2/tPGK1):
Enzyme digestion system:
pSB1C3-pTDH3/ tPGK1 | 20 μL |
BamHI | 3 μL |
buffer | 5 μL |
ddH2O | 22 μL |
Seamless cloning system:
pSB1C3-pTDH3/ tPGK1 | 2 μL |
TgMTP1t2 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K3126017 (pSB1C3-pTEF2/kanR/tENO2):
Enzyme digestion system:
pSB1C3-pTEF2/tENO2 | 20 μL |
BamHI | 3 μL |
buffer | 5 μL |
ddH2O | 22 μL |
Seamless cloning system:
pSB1C3-pTEF2/tENO2 | 2 μL |
kanR | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
2019.6.20 ~ 6.30
Construction of plasmids for the translation and expression of genes in S. cerevisiae:
pSB1C3-BBa_K3126018 (pSB1C3- hexa-His/ kanR)
The sequence of pSB1C3-BBa_K3126018
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126019 (pSB1C3-nixA/ kanR)
The sequence of pSB1C3-BBa_K3126019
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126020 (pSB1C3-TgMTP1t2/ kanR)
The sequence of pSB1C3 BBa_K3126020
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
The resistance gene(kanR) was linked to the target gene(hexa-His, nixA, TgMTP1t2) to screen the engineered yeast and the original yeast.
pSB1C3-BBa_K3126018 (pSB1C3-hexa-His/ kanR):
Enzyme digestion system:
pSB1C3-BBa_K3126014 | 25 μL |
SpeI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
pSB1C3-BBa_K3126017 | 25 μL |
XbaI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
The enzyme system we use:
BBa_K3126014 linear plasmid | 2 μL |
kanR part | 6 μL |
T4 DNA Ligase | 0.5 μL |
T4Buffer | 1 μL |
ddH2O | 0.5 μL |
pSB1C3-BBa_K3126019 (pSB1C3-nixA/ kanR)
Enzyme digestion system:
pSB1C3-BBa_K3126015 | 25 μL |
SpeI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
pSB1C3-BBa_K3126017 | 25 μL |
XbaI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
The enzyme system we use:
pSB1C3-BBa_K3126015 linear plasmid | 2 μL |
kanR part | 4 μL |
T4 DNA Ligase | 0.5 μL |
T4Buffer | 1 μL |
ddH2O | 0.5 μL |
pSB1C3-BBa_K3126020 (pSB1C3-TgMTP1t2/ kanR)
Enzyme digestion system:
pSB1C3-BBa_K3126016 | 25 μL |
SpeI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
pSB1C3-BBa_K3126017 | 25 μL |
XbaI | 3 μL |
PstI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 14 μL |
The enzyme system we use:
pSB1C3-BBa_K3126016 linear plasmid | 2 μL |
kanR part | 6 μL |
T4 DNA Ligase | 0.5 μL |
T4Buffer | 1 μL |
ddH2O | 0.5 μL |
2019.7.1 ~ 7.10
Construction of plasmids for the translation and expression of genes in S. cerevisiae:
pSB1C3-BBa_K3126021 (pSB1C3- hexa-His/ nixA/ kanR)
The sequence of pSB1C3-BBa_K3126021
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126022 (pSB1C3- nixA/ TgMTP1t2/ kanR)
The sequence of pSB1C3-BBa_K3126022
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
pSB1C3-BBa_K3126023 (pSB1C3- hexa-His/ nixA/ TgMTP1t2/ kanR)
The sequence of pSB1C3-BBa_K3126023
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGGTCTCATGTCTATCCCAGAAACTCAAAAAGGTGTTATCTTCTACGAATCCCCCTTGCCAACAGGGAGTTCTTCAGAGACATGGAGGCTCAAAACGAAATTATTGACAGCCTAGACATCAATAGTCATACAACAGAAAGCGACCACCCAACTTTGGCTGATAATAGCGTATAAACAATGCATACTTTGTACGTTCAAAATACAATGCAGTAGATATATTTATGCATATTACATATAATACATATCACATAGGAAGCAACAGGCGCGTTGGACTTTTAATTTTCGAGGACCGCGAATCCTTACATCACACCCAATCCCCCACAAGTGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTCCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGACACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAAATTTTTTTTTTTGATTTTTTTCTCTTTCGATGACCTCCCATTGATATTTAAGTTAATAAACGGTCATCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGCTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGTTTTAATTACAAAGGATCCATGAGATTCCCTAGTATCTTCACCGCCGTCTTGTTCGCTGCTAGTTCTGCTTTGGCTGCTCCAGTCAACACTACCACCGAAGACGAAACCGCTCAGATCCCAGCTGAAGCCGTCATCGGTTACCTTGACCTTGAGGGTGACTTCGATGTCGCCGTCCTTCCATTCTCTAACAGTACCAACAACGGTCTTTTGTTCATTAACACCACCATTGCTTCTATCGCCGCTAAGGAGGAGGGTGTCTCTCTTGACAAGAGACATCACCACCACCATCACAGTGCTAAGTCTAGTTTCATCTCTACCACCACTACCGACCTTACCTCTATCAACACCAGTGCCTACAGTACCGGTTCTATCTCTACCGTCGAAACCGGAAACAGAACCACCTCTGAGGTCATCTCTCATGTCGTCACCACCTCTACCAAGCTTAGTCCTACCGCCACCACCAGTCTTACCATCGCTCAAACTTCTATTTATAGTACCGACTCTAATATTACCGTCGGTACCGACATCCACACCACCAGTGAAGTCATCTCTGACGTCGAAACCATCTCTAGGGAAACCGCCTCTACCGTTGTTGCCGCCCCAACTTCTACCACTGGTTGGACCGGTGCCATGAACACTTACATCTCTCAATTCACTTCTTCTAGTTTTGCTACTATTAACTCTACCCCTATCATCTCTTCTAGTGCCGTCTTCGAAACCAGTGACGCCTCTATCGTCAACGTCCATACCGAGAACATCACCAACACTGCTGCCGTTCCATCTGAGGAGCCTACCTTCGTCAACGCCACTAGAAACTCTCTTAATTCTTTTTGTTCTTCTAAACAACCATCTTCTCCTAGTTCTTATACTTCTTCTCCTTTGGTTTCTTCTTTGTCTGTTTCTAAGACCTTGTTGTCTACCTCTTTCACCCCTTCTGTCCCAACTTCTAATACTTATATTAAGACCAAGAACACTGGTTACTTCGAGCACACTGCCTTGACCACCTCTAGTGTTGGATTGAATAGTTTCTCTGAAACCGCCGTCTCTAGTCAAGGTACCAAGATTGACACCTTTTTGGTTTCTAGTTTGATCGCCTACCCATCTTCTGCCTCTGGTTCTCAGCTTAGTGGTATCCAGCAAAATTTTACCTCTACTTCTTTGATGATCAGTACCTACGAGGGTAAGGCCAGTATCTTCTTCTCTGCCGAGTTGGGTTCTATTATTTTTTTGTTGCTTAGTTATTTGTTGTTTTAAAACGGGTACTGTACAGTTAGTACATTGAGTCGAAATATACGAAATTATTGTTCATAATTTTCATCCTGGCTCTTTTTTTCTTCAACCATAGTTAAATGGACAGTTCATATCTTAACTCTAATAATACTTTTCTAGTTCTTATCCTTTTCCGTCTCACCGCAGATTTTATCATAGTATTAAATTTATATTTTGTTCGTAAAAAGAAAAATTTGTGAGCGTTACCGCTCGTTTCATTACCCGAAGGCTGTTTCAGTAGACCACTGATTAAGTAAGTAGATGAAAAAATTTCATCACCATGAAAGAGTTCGATGAGAGCTACTTTTTCAAATGCTTAACAGCTAACCGCCATTCAATAATGTTACGCTCTCTTCATTCTGCGGCTACGTTATCTAACAAGAGGTTTTACTCTCTCATATCTCATTCAAATAGAAAGAACATAATCAAAAGGATCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACACATGCAGTGATGCACGCGCGATGGTGCTAAGTTACATATATATATATATATATATATATATATATATATAGCCATAGTGATGTCTAAGTAACCTTTATGGTATATTTCTTAATGTGGAAAGATACTAGCGCGCGCACCCACACACAAGCTTCGTCTTTTCTTGAAGAAAAGAGGAAGCTCGCTAAATGGGATTCCACTTTCCGTTCCCTGCCAGCTGATGGAAAAAGGTTAGTGGAACGATGAAGAATAAAAAGAGAGATCCACTGAGGTGAAATTTCAGCTGACAGCGAGTTTCATGATCGTGATGAACAATGGTAACGAGTTGTGGCTGTTGCCAGGGAGGGTGGTTCTCAACTTTTAATGTATGGCCAAATCGCTACTTGGGTTTGTTATATAACAAAGAAGAAATAATGAACTGATTCTCTTCCTCCTTCTTGTCCTTTCTTAATTCTGTTGTAATTACCTTCCTTTGTAATTTTTTTTGTAATTATTCTTCTTAATAATCCAAACAAACACACATATTACAATAGGATCCATGATTAAGTACCATGTCATGAAAGAAGGTTACATCTTGACTGTTTTTAAAAATGAAAGGTTGAGTTGGTTGCCATATATCGCCATCGTCATTTTGTTGCACGTTATTGGTTTCTCTTTCTTGTGGATCGCCGGTAAGGACCATCACATTTTGTTCGGTATGGGTATCCTTGCCTACACTCTTGGTTTGAGGCATGCCTTTGATGCCGACCATATCGCCGCTATCGACAACACCGTTAGAAAGTTGTTGCAGCAGAGAAAGGACCCATCTGGTGTCGGTTTCTACTTCTCTATTGGACATTCTTCTGTCGTCTTCTTGATGGCCGTTTTCCTTGGTGTCTCTGTCAAGTGGGCCAAGGACGAACTTCCTCACTTCCAAGATATCGGAGGTACCATTGGAACCCTTGTTTCTGGTTTTTTCTTGGTTCTTATTGGAGTTCTTAATCTTATTATTCTTATTTCTTTGATTAACTTGTTCGCCAAGTTGAGGAGGGAACACATCGAAGAAGCTGAGGTCGATGCCTTGTTGGAGTCTAGGGGTCTTGTCTCTAGGTTCGTCGGACCATACTTTAAGTTGATCACCAGAAGTTGGCACGTCTTGCCTCTTGGATTCCTTTTCGGATTGGGATTCGACACCGCTTCTGAGATCGCCTTGCTTGCCCTTAGTAGTGGAGCCTCTCAGCAAGCTATCTCTTTCATTGGTATTTTGTCTTTGCCTATCTTGTTCGCCAGTGGTATGAGTCTTCTTGACACCCTTGACGGTGTCGTCATGAAGTACGCCTACAACTGGGCCTTCTTCAACCCTATCAGAAAAATCTATTACAACATTACCATTACCGCCATCTCTGTTATGGCCGCCCTTGTTATCGGTATGATCGAATTGCTTCAAATTCTTGCCGACAAACTTGACTTGCATGGAGCCTTCTGGGCCTTCATTGGAAGTATCGAGTTCGATTATTTGGGTTATATCTTGGTTGCCTTGTTTCTTATTACTTGGTTGATCAGTTCTTTGATTTGGAAATTTGGAAGAATTGAACACAAATGGAGTAGATAAGGATCCATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGAGGCAGTTCGAGTTTATCATTATCAATACTGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAAGAACTTAGTTTCGAATAAACACACATAAACAAACAAAGGATCCATGGAGAGTTCTTCTCATATTATTGAAGTCAACGGAGGTAGATCTGACGAGGAAAGAAGGGCTGTCGCTAGTAAGGTCTGCGGAGAAGCTCCTTGCGGTTTCAGTGACGCTAAGAACGCTTCTGGTGACGCCCAAGAAAGGAACGCCTCTATGAGGAAATTGTGCATCGCCGTCGTCTTGTGCCTTGTCTTCATGTCTGTCGAGGTCGTTGGTGGTGTCAAGGCCAACAGTTTGGCCATCATGACCGATGCCGCTCATCTTTTGAGTGACGTTGCCGCTTTCGCCATCTCTTTGTTCAGTCTTTGGGCTGCCGGTTGGGAGGCTACCCCAAGGCACACCTACGGATTCTTCAGAATCGAAATTCTTGGAGCTCTTGTTTCTATCCAATTGATTTGGTTGCTTACTGGTATCCTTGTTTACGAGGCCATCATTAGACTTTTGACCGAAACCTCTGAGGTCAACGGTTTCTTGATGTTCGCTGTCGCCACTTTCGGTTTGCTTGTCAACATTATCATGGCTGTCATGCTTGGTCACGACCATGACAATGGTCACGGTCATAGTCACGGTGAGGACAAACACGATGAAGCCCATGGTGACGTCACTGAACAGCTTCTTGAAAAGCCAAAACAGCAGAAGGAAAAAAAGAAGAGGAACATCAACGCCCAAGGTGCCTATTTGCACGTCTTGGGTGACTCTATCCAGTCTGTCGGAGTCATGATCGGTGGTGCTGCTATCTGGTACAATCCAAAGTGGAAGATCATTGACTTGATCTGCACCTTGGTCTTCTCTGTCATCGTCTTGGGAACCACCATCAATATGATCAGAAGTATTCTTGAGGTCTTGATGGAGAGTACCCCAAGAGAAATCGACGCCACCAAGCTTGAGAAGGGTCTTCTTGAGATGGAGGAGGTCGTCGCTGTTCACGAATTGCACATCTGGGCCTCTACCGTTGGTAAGGTCTTGCTTGCTTGCCATGTCAACGCCAGACCAGATGCCGATGCCGATATGGTCCTTAACAAAGTCGTTGACTATATTAGGAGAGAATATAACATTTCTCATGTTACTATCCAAATTGAAAGATAAGGATCCATTGAATTGAATTGAAATCGATAGATCAATTTTTTTCTTTTCTCTTTCCCCATCCTTTACGCTAAAATAATAGTTTATTTTATTTTTTGAATATTTTTTATTTATATACGTATATATAGACTATTATTTATCTTTTAATGATTATTAAGATTTTTATTAAAAAAAAATTCGCTCCTCTTTTAATGCCTTTATGCAGTTTTTTTTTCCCATTCGATATTTCTATGTAGGCCTTACTAGAGTTGATAGGTCAAGATCAATGTAAACAATTACTTTGTTATGTAGAGTTTTTTTAGCTACCTATATTCCACCATAACATCAATCATGCGGTTGCTGGTGTATTTACCAATAATGTTTAATGTATATATATATATATATATATGGGGCCGTATACTTACATATAGTAGATGTCAAGCGTAGGCGCTTCCCCTGCCGGCTGTGAGGGCGCCATAACCAAGGTATCTATAGACCGCCAATCAGCAAACTACCTCCGTACATTCATGTTGCACCCACACATTTATACACCCAGACCGCGACAAATTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAACGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATATGGGAAAGGAGAAGACCCACGTCAGTAGACCAAGGCTTAACAGTAACATGGACGCCGACTTGTACGGATACAAGTGGGCCAGAGACAACGTCGGTCAGTCTGGAGCTACCATCTATAGACTTTACGGAAAGCCAGACGCCCCAGAGTTGTTCCTTAAGCACGGTAAGGGATCTGTTGCCAACGACGTCACCGATGAGATGGTTAGACTTAACTGGCTTACCGAGTTCATGCCACTTCCAACCATCAAGCACTTCATTAGAACCCCAGATGACGCTTGGTTGTTGACCACTGCCATCCCCGGTAAGACCGCCTTTCAAGTTTTGGAAGAGTACCCAGACTCTGGAGAGAACATCGTTGACGCCTTGGCCGTCTTCCTTAGGAGGTTGCACTCTATCCCAGTTTGCAACTGCCCATTCAATAGTGATAGAGTCTTCAGACTTGCCCAAGCCCAGTCTAGGATGAACAACGGATTGGTCGATGCCTCTGACTTCGACGACGAGAGAAATGGATGGCCAGTCGAGCAAGTTTGGAAGGAGATGCATAAGCTTTTGCCTTTTTCTCCAGACAGTGTTGTCACCCACGGTGACTTTAGTTTGGACAACCTTATTTTCGACGAGGGTAAATTGATCGGTTGCATCGACGTCGGTAGAGTTGGTATCGCCGATAGATACCAAGACTTGGCCATCTTGTGGAACTGCTTGGGTGAGTTCTCTCCAAGTCTTCAAAAAAGATTGTTCCAAAAATATGGTATTGACAACCCAGATATGAATAAATTGCAATTTCATTTGATGTTGGATGAGTTTTTCTAAATAACTTCGTATAATGTATGCTATACGAAGTTATGGATCCAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATAAAGGGTCAAATCGTTGGTAGATACGTTGTTGACACTTCTAAATAAGAGACCTACTAGTAGCGGCCGCTGCA
We obtained the fragments to be inserted by PCR, then combine several target genes by seamless cloning.
pSB1C3-BBa_K3126021 (pSB1C3- hexa-His/ nixA/ kanR):
The PCR system of pGPM1-nixA-tTDH1:
(P-nixA-T_F
CGAAGGCTTTAATTTGCAGGGCCAAACTTTTCGGTTAACA
P-nixA-T_R
ACCTATCAACTCTAGTAAGGCCTCGTTCAGGGTAATATATTTT)
pSB1C3-BBa_K3126015/SpeⅠ PstⅠ | 1 μL |
P-nixA-T_F | 2 μL |
P-nixA-T_R | 2 μL |
2×PCR mix | 20 μL |
ddH2O | 15 μL |
Enzyme digestion system:
pSB1C3-BBa_K3126018 | 15 μL |
StuI | 3 μL |
buffer | 5 μL |
ddH2O | 27 μL |
Seamless cloning system:
pSB1C3-BBa_K3126018/StuⅠ | 2 μL |
pGPM1-nixA-tTDH1 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K3126022 (pSB1C3- nixA/ TgMTP1t2/ kanR)
The PCR system of pTDH3-TgMTP1t2-tPGK1
pSB1C3-BBa_K3126016/SpeⅠ PstⅠ | 1 μL |
P-TgMTP1t2-T_F | 2 μL |
P-TgMTP1t2-T_R | 2 μL |
2×PCR mix | 20 μL |
ddH2O | 15 μL |
Enzyme digestion system:
pSB1C3-BBa_K3126021 | 15 μL |
StuI | 3 μL |
buffer | 5 μL |
ddH2O | 27 μL |
Seamless cloning system:
pSB1C3-BBa_K3126021/StuⅠ | 2 μL |
pTDH3-TgMTP1t2-tPGK1 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K3126023 (pSB1C3- hexa-His/ nixA/ TgMTP1t2/ kanR)
Enzyme digestion system:
pSB1C3-BBa_K3126021 | 15 μL |
StuI | 3 μL |
buffer | 5 μL |
ddH2O | 27 μL |
Seamless cloning system:
pSB1C3-BBa_K3126021/StuⅠ | 2 μL |
pTDH3-TgMTP1t2-tPGK1 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
2019.7.20 ~ 7.25
The plasmid is digested with Bsa1 to get the fragment to be integrated.
pSB1C3- hexa-His- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126018 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
pSB1C3-nixA- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126019 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
pSB1C3-TgMTP1t2- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126020 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
pSB1C3-hexa-His-nixA- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126021 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
pSB1C3-nixA-TgMTP1t2- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126022 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
pSB1C3-hexa-His-nixA-TgMTP1t2- kanR/ BsaⅠ
Enzyme digestion system:
pSB1C3-BBa_K3126023 | 25μL |
BsaI | 3 μL |
buffer | 5 μL |
ddH2O | 17 μL |
2019.7.25 ~ 8.5
The fragments were transformed into S. cerevisiae to express the corresponding protein.
2019.8.25 ~ 9.10
Adsorption of Nickel ion by S. cerevisiae
1.Culture yeast for a period of time, measure its OD600
2. Convert the required dry weight volume according to OD600
3.Add the corresponding volume of yeast solution into 50mL centrifuge tube, centrifugate at 8000 rpm for 5 min, discard the supernatant and enrich the cells
4.Prepare 21 bottles of 50mL 15mg / L nickel ion solution and 21 bottles of 50mL 200mg / L nickel ion solution, put the put the six kinds of engineered yeast and original yeast into the solution respectively, and culture them in a shaker at 30℃
5.Take the engineered yeast solution every interval to measure the nickel ion concentration
6.After about half an hour of adsorption, the equilibrium is reached
7.Draw the curve about the enrichment of Ni2+ by yeast.
Improvement
2019.9.20 ~ 2019.9.30
[Protocols]
1.Digested the pSB1C3-BBa_K2652001 circular plasmid with BamHI.
2.Amplified our target gene BBa_K3126026 (His-Tag-nikABCDE- His-Tag) using PCR technology.
3.Recovered the digested part and inserted BBa_K3126026 (His-Tag-nikABCDE-His-Tag) into pSB1C3-BBa_K2652001 by seamless cloning, and the mixed system was reacted at 37℃ for 30 minutes.
4.converted all of the products of the above seamless cloning system to DH5α.
5.Applied DH5α to the plasmid to a plate containing chloramphenicol resistance.
[Enzyme Digestion System]
pSB1C3-BBa_K2652001 circular plasmid | 20 μL |
BamHI | 3 μL |
Buffer | 5 μL |
ddH2O | 27 μL |
[PCR System]
Template | 1 μL |
Primer-F | 2 μL |
Primer-R | 2 μL |
2×Rapid Taq Master Mix | 20 μL |
ddH2O | 27 μL |
[PCR System]
1 | 95℃ | 30s |
2 | 95℃ | 8s |
3 | 60℃ | 30s |
4 | 72℃ | 2min30s |
5 | 72℃ | 5min |
6 | 4℃ | ∞ |
(2、3、4-thirty cycles)
[Seamless cloning system]
pSB1C3-BBa_K2652001 circular plasmid/ BamHⅠ | 3 μL |
His-Tag-nikABCDE- His-Tag | 4 μL |
Buffer | 2 μL |
ExnaseⅡ | 1 μL |
Enzyme Digestion picture:
[Adsorption Experiment of E.coli]
1. Culture E.coli for a period of time, measure its OD600
2. Convert the required dry weight volume according to OD600
3.Add the corresponding volume of bacterial solution into 50mL centrifuge tube, centrifugate at 8000 rpm for 12 min, discard the supernatant and enrich the cells (more than 30mL is divided into two centrifuges);
4.Prepare 6 bottles of 50mL 30mg / L nickel ion solution, put the E.coli into the solution respectively, and culture them in a shaker at 37℃.
5.Take 1.5mL solution every 2 min in the centrifuge tube, take 5 times, and then take 3 times every 5min;
6.Using dimethylglyoxime spectrophotometry to measure the nickel ion concentration of the above samples;
7. Draw the relation curve about time and nickel ion concentration.
The sequence of BBa_K2652001
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCAGGAAGAAAC
The sequence of BBa_K3126026
CATCATCATCATCATCACATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAGAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATGGCACCCAGCAGGTCGCCGACACCCTGTTTGCCCCTTCTGTGCCCTACGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGTTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGTCGTTTTGGCATGATTTTCCACCGCACCTGGGGCGCGCCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCGACCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACATCCCCTACGCGCCGATCGCCACCGAAATTCCGTTCGAACAGATTAAACCGGTGAAACCTTAAATGTTGCGTTACGTATTACGCCGCTTTCTGCTGCTGATCCCGATGGTGCTCGCCGCCTCGGTGATCATTTTTCTGATGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCTCACTTCCGTACCGCTCGGTATCTGGGCGGCGCGCCATCGCGACCGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTACGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCTGCGCGGCCTGAACGACAAACAGACCGAACGTCGCCACATCCTGCGCAATGCCTCGCTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGTGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAGTGAACTTTTTCCTCTCTTCCCGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGACCCAGCCGCTGCAAATGTTCTGGCCGGGGCTGGCGCTGTTTATCAGCGTGATGGCCTTTAACCTGGTGGGTGACGCACTGCGCGATCATCTGGACCCTCATCTGGTGACGGAGCACGCACACTAAATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGAATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGTGGATTTAACGGAAAACATCAACATCAGGCGGTGCTGAATAACGTTTCCCTGACCCTGAAAAGCGGCGAAACCGTCGCCCTGCTGGGGCGCAGCGGCTGCGGGAAAAGTACCCTCGCGCGGTTGCTGGTGGGTTTAGAATCGCCCGCGCAGGGGAATATTAGCTGGCGTGGCGAACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTCACCACCACCACCACCACTAA
The sequence of pSB1C3-BBa_K3126026
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCCATCATCATCATCATCACATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGTGGCGTTTGAAACTGGCGATATCGACCTGCTGTACGGAAACGAAGGGTTATTACCGCTCGATACCTTCGCCCGCTTTAGCCAGAATCCGGCTTACCACACCCAACTGTCACAGCCGATCGAAACCGTGATGCTGGCGCTCAATACCGCCAAAGCCCCCACCAACGAGCTGGCAGTACGTGAAGCTCTTAATTACGCGGTAAACAAAAAATCGCTGATTGATAACGCGTTGTATGGCACCCAGCAGGTCGCCGACACCCTGTTTGCCCCTTCTGTGCCCTACGCCAACCTCGGCCTGAAACCGAGCCAGTACGATCCGCAAAAAGCGAAAGCGTTGCTGGAAAAAGCCGGTTGGACGCTGCCTGCGGGCAAAGACATCCGCGAGAAAAATGGTCAGCCGCTGCGCATTGAACTTTCGTTCATCGGCACCGATGCGTTAAGCAAATCGATGGCGGAAATCATTCAGGCTGATATGCGCCAGATTGGCGCAGATGTCTCGCTGATTGGCGAAGAAGAGAGCAGTATCTATGCTCGTCAGCGCGACGGTCGTTTTGGCATGATTTTCCACCGCACCTGGGGCGCGCCATATGATCCACACGCCTTCCTCAGTTCAATGCGCGTACCGTCACACGCTGACTTCCAGGCACAGCAAGGATTAGCCGACAAACCGCTGATTGATAAAGAGATCGGCGAAGTGCTGGCGACCCATGACGAAACGCAACGTCAGGCGCTGTATCGCGACATTCTGACCCGTCTGCATGACGAGGCGGTTTATCTGCCTATCAGTTACATCTCAATGATGGTGGTATCAAAACCGGAGCTGGGTAACATCCCCTACGCGCCGATCGCCACCGAAATTCCGTTCGAACAGATTAAACCGGTGAAACCTTAAATGTTGCGTTACGTATTACGCCGCTTTCTGCTGCTGATCCCGATGGTGCTCGCCGCCTCGGTGATCATTTTTCTGATGCTGCGCCTCGGTACCGGCGACCCGGCGCTCGATTATTTGCGTCTGTCTAACCTGCCGCCGACGCCGGAGATGCTGGCCTCTACCCGCACCATGCTGGGACTGGATCAGCCGCTGTACGTCCAGTACGGCACCTGGTTGTGGAAGGCGCTGCATCTTGACTTTGGTATCTCATTCGCCAGCCAACGCCCGGTACTGGACGATATGCTGAACTTCCTGCCCGCCACGCTGGAACTTGCAGGTGCGGCGCTGGTATTAATTCTGCTCACTTCCGTACCGCTCGGTATCTGGGCGGCGCGCCATCGCGACCGTCTGCCGGATTTCGCCGTACGTTTCATCGCGTTTCTTGGCGTGTCGATGCCTAACTTCTGGCTGGCGTTTTTACTGGTGATGGCGTTTTCGGTGTATCTGCAATGGCTACCCGCGATGGGTTACGGCGGCTGGCAGCACATCATTTTGCCTGCGGTTTCCATTGCCTTTATGTCGCTGGCGATTAACGCGCGTTTACTGCGCGCCAGTATGCTGGACGTCGCCGGTCAGCGTCACGTCACCTGGGCGCGTCTGCGCGGCCTGAACGACAAACAGACCGAACGTCGCCACATCCTGCGCAATGCCTCGCTGCCGATGATCACCGCCGTGGGGATGCATATCGGCGAACTGATTGGCGGGACGATGATTATCGAAAACATCTTTGCCTGGCCGGGCGTCGGGCGCTATGCGGTGTCGGCGATTTTTAACCGTGACTATCCGGTGATCCAGTGCTTTACGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGTCGTCATGAAGGAGCGCACGCGTGAGTGAACTTTTTCCTCTCTTCCCGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGCGGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTGGCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGACCCAGCCGCTGCAAATGTTCTGGCCGGGGCTGGCGCTGTTTATCAGCGTGATGGCCTTTAACCTGGTGGGTGACGCACTGCGCGATCATCTGGACCCTCATCTGGTGACGGAGCACGCACACTAAATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGATGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTGGTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGAATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGTGGATTTAACGGAAAACATCAACATCAGGCGGTGCTGAATAACGTTTCCCTGACCCTGAAAAGCGGCGAAACCGTCGCCCTGCTGGGGCGCAGCGGCTGCGGGAAAAGTACCCTCGCGCGGTTGCTGGTGGGTTTAGAATCGCCCGCGCAGGGGAATATTAGCTGGCGTGGCGAACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACGCTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAATTAACCTTTTCCTCTGACGCCGGACGTGTGCTACAAAACGCGGTATTACCCGCATTCCCCGTGCGCCGTCGCACCACAGAAAAGGTTCACCACCACCACCACCACTAAGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCA
Characterization
2019.9.10 ~ 2019.9.20
We used the pSB1C3-BBa_K2652001 from last year and digested with BamH I enzyme.
The pSB1C3 digestion system we used:
pSB1C3-BBa_K2652001 | 15μL |
BamHI | 3 μL |
Cutsmart Buffer | 5 μL |
ddH2O | 27 μL |
pSB1C3-BBa_K3126028(pSB1C3-BBa_K2652001/ BBa_E0032)
pSB1C3-BBa_K3126029(pSB1C3-BBa_K2652001/ BBa_E0034)
pSB1C3-BBa_K3126030(pSB1C3-BBa_K2652001/GFP)
We obtained the fragments to be inserted by PCR, then combine several target genes by seamless cloning.
The PCR system of BBa_E0032 and BBa_E0034:
BBa_E0032_F
AGGGAGCTTTAAATCGGATCCatggtgagcaagggcgagga
BBa_E0032_R
TGCCTGGCTCTAGTAGGATCCTTATTAAGCTACTAAAGCGT
pSB1C3- BBa_E0032/ BamHI | 1μL |
BBa_E0032_F | 2 μL |
BBa_E0032_R | 2 μL |
2×PCR mix | 20 μL |
ddH2O | 27 μL |
BBa_E0034_F
AGGGAGCTTTAAATCGGATCCatggtgagcaagggcgagga
BBa_E0034_R
TGCCTGGCTCTAGTAGGATCCTTATTAAACAGCAGCAGCGT
pSB1C3- BBa_E0034/ BamHI | 1μL |
BBa_E0034_F | 2 μL |
BBa_E0034_R | 2 μL |
2×PCR mix | 20 μL |
ddH2O | 15 μL |
pSB1C3-GFP | 1μL |
GFP_F | 2 μL |
GFP_R | 2 μL |
2×PCR mix | 20 μL |
ddH2O | 15 μL |
Seamless cloning system:
pSB1C3-BBa_K2652001/BamHI | 2μL |
BBa_E0032 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K2652001/BamHI | 2μL |
BBa_E0034 | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
pSB1C3-BBa_K2652001/BamHI | 2μL |
GFP | 4 μL |
5×CEⅡ Buffer | 2 μL |
Exnase Ⅱ | 1 μL |
ddH2O | 1 μL |
The sequence of BBa_K2652001
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCAGGAAGAAAC
The sequence of BBa_E0032
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
The sequence of BBa_K3126028
TTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataaGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
The sequence of pSB1C3-BBa_K3126028
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataaGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCA
The sequence of BBa_E0034
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataa
The sequence of BBa_K3126029
TTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataaGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
The sequence of pSB1C3-BBa_K3126029
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataaGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCA
The sequence of GFP
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
The sequence of BBa_K3126030
TTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAGGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
The sequence of pSB1C3-BBa_K3126030
GTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGCCCTAGGTATTATGCTAGCACATTTCCAACACTAACCCAAGGGAGCTTTAAATCGGATCC ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA GGATCCtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgCTCGAGTACTAGTAGCGGCCGCTGCA
option date
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Method-Protocol -
Notebook -
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Improvement-notebook -
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2019.9.20 ~ 2019.9.30
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Characterization-notebook -
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2019.9.10 ~ 2019.9.20
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