Team:Guelph/Composite Part

Here are our composite parts. To see them in action visit our Results Page!

BioBrick	Type	Name	Description	Length
BBa_K3189005	Composite	Tet-Regulated VioC	A DNA construct that has the necessary components to express and detect VioC under the control of tetracycline. Components include a TetO promoter, RBS, VioC, a terminator and an s-tag.	2306
BBa_K3189006	Composite	Tet-Regulated VioC	The same as BBa_K3189005 however, with additional linker sequences between the tetO->RBS and T7Term->S-Tag	2354
BBa_K3189013	Composite	Lac-regulated VioABE	This composite part puts VioABE under the control of the Lac operon. When induced with IPTG this metabolizes tryptophan into tryptophan into protodeoxy-violaceinic acid which spontaneously degrades into prodeoxyviolacein, a green pigment.	6605
BBa_K3189014	Composite	Tet Controlled Stable amilCP	This is a tet-regulated stable amilCP based on the E. coli codon optimized BioBrick variant BBa_K2669002. This is the part that was placed in our backbone for our characterization tests.	813
BBa_K3189015	Reporter	Tet Controlled amilCP	This is a tet-regulated amilCP based BioBrick variant BBa_K1343022 that our team improved for the gold requirement. BBa_K1343022 had its amilCP PCR amplfied out and placed into the pJKR backbone to create a version that created amilCP when Tetracycline is present and not when the AHL-luxR dimer is present.	1806

University of Guelph iGEM 2019

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★ ALERT!

This page is used by the judges to evaluate your team for the medal criterion or award listed below.

Delete this box in order to be evaluated for this medal criterion and/or award. See more information at Instructions for Pages for awards.

Composite Parts

A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. BBa_I13507 is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.

New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).

Note

This page should list all the composite parts your team has made during your project and include direct links to your Parts main pages on the Registry. You must add all characterization information for your parts on Parts Main Page on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!

Best Composite Part Special Prize

To be eligible for this award, this part must be well documented on the part's Main Page on the Registry. If you have a part you wish to nominate your team for this special prize, make sure you add your part number to your judging form and delete the alert box at the top of this page.

Please note: Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize.