Team:Guelph/Parts

Here is a comprehensive list of all of the BioBricks we created this year!

BioBrick	Type	Name	Description	Length
BBa_K3189000	Coding	VioC	The gene VioC produces Violacein synthase required for the metabolism of tryptophan into a pink or violet (if VioD is present) pigment.	1290
BBa_K3189001	Regulatory	Phage Lambda-TetO	A phage lambda promoter sequence that has a binding site that allows tetR to bind and repress transcription.	74
BBa_K3189002	Tag	S-Tag	An oligopeptide tag that can allow for the detection of the gene directly upstream of this sequence.	90
BBa_K3189003	Terminator	T7 Terminator	Wild type terminator from T7 bacteriophage.	48
BBa_K3189004	Regulatory	TetR	Tetracycline repressor that can bind to a TetO sequence to inhibit transcription of a desired gene.	624
BBa_K3189005	Composite	Tet-Regulated VioC	A DNA construct that has the necessary components to express and detect VioC under the control of tetracycline. Components include a TetO promoter, RBS, VioC, a terminator and an s-tag.	2306
BBa_K3189006	Composite	Tet-Regulated VioC	The same as BBa_K3189005 however, with additional linker sequences between the tetO->RBS and T7Term->S-Tag	2354
BBa_K3189007	RBS	Shine-Delgarno Sequence	A sequence that allows the ribosome to bind and initiate protein translation.	36
BBa_K3189008	Regulatory	LacI + Promoter	The repressor sequence and its promoter that control the transcription of genes that govern the Lac system.	1161
BBa_K3189009	Regulatory	Lac Operator	The regulatory sequence that can be bound to Lac I to inhibit expression of a given gene.	40
BBa_K3189010	Coding	VioA	The gene VioA produces flavin-dependent L-tryptophan oxidase. VioA along with VioB and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.	1290
BBa_K3189011	Coding	VioB	The gene VioB produces 2-imino-3-(indol-3-yl)-propanoate dimerase. VioB along with VioA and VioE are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.	3030
BBa_K3189012	Coding	VioE	The gene VioE produces a tryptophan oxidase. VioE along with VioA and VioB are all required for the metabolism of tryptophan into protodeoxy-violaceinic acid, the precursor to the violacein pigment.	606
BBa_K3189013	Composite	Lac-regulated VioABE	This composite part puts VioABE under the control of the Lac operon. When induced with IPTG this metabolizes tryptophan into tryptophan into protodeoxy-violaceinic acid which spontaneously degrades into prodeoxyviolacein, a green pigment.	6605
BBa_K3189014	Composite	Tet Controlled Stable amilCP	This is a tet-regulated stable amilCP based on the E. coli codon optimized BioBrick variant BBa_K2669002. This is the part that was placed in our backbone for our characterization tests.	813
BBa_K3189015	Reporter	Tet Controlled amilCP	This is a tet-regulated amilCP based BioBrick variant BBa_K1343022 that our team improved for the gold requirement. BBa_K1343022 had its amilCP PCR amplfied out and placed into the pJKR backbone to create a version that created amilCP when Tetracycline is present and not when the AHL-luxR dimer is present.	1806