Composite parts
For the construction of our composite BioBricks, we used the 3A assembly method of cloning. The fusion protein mCherry which is a basic part (
BBa_J06504) already available in the registry is used to create composite parts of P1-mCherry (
BBa_K3171172) and
E. coli optimized pTet-mCherry (
BBa_K3171175). The reporter mCherry was chosen because of its higher suitability in
V. natriegens.
| # |
Name |
Type |
Description |
Designer |
| 1 |
BBa_K3171172 |
Composite |
V. natriegens native promoter P1-mCherry |
RUG, 2019 |
| 2 |
BBa_K3171175 |
Composite |
pTet-mCherry optimized in E. coli |
RUG, 2019 |
Table 1. BioBrick Parts submitted by University of Groningen iGEM 2019 team. All BioBricks were constructed by the team members engaged in the wet lab; Sander de Weerd, Michelle Scharte, Nurul Wirusanti, Yashika Venkatesh, Dasha Vedernikova, Sophie Schertlen, Lieke van Iersel.
