Team:Gaston Day School/Contribution
Characterization
BBa_K174015
When combined with an RBS (B0036), RFP (E1010), and a double terminator (B0014), the Gaston Day School 2019 team has shown that this promoter is sensitive to cadmium down to 0.25M (p<0.01). Work by earlier Gaston Day School teams had shown that the promoter did work, but the data was not clean and the minimum level of detection was significantly higher (approximately 10mM). Cultures were grown for 24 hours in the presence of 0, 0.25, or 0.75 mM cadmium. Absorbance was measured using a Vernier SpectroVis Plus and data was collected and analyzed using Microsoft Excel’s t-test function. Results are reported as absorbance at 517 nm normalized using absorbance at 700 nm.
Characterization
| BBa_K174015 |
|---|
When combined with an RBS (B0036), RFP (E1010), and a double terminator (B0014), the Gaston Day School 2019 team has shown that this promoter is sensitive to cadmium down to 0.25M (p<0.01). Work by earlier Gaston Day School teams had shown that the promoter did work, but the data was not clean and the minimum level of detection was significantly higher (approximately 10mM). Cultures were grown for 24 hours in the presence of 0, 0.25, or 0.75 mM cadmium. Absorbance was measured using a Vernier SpectroVis Plus and data was collected and analyzed using Microsoft Excel’s t-test function. Results are reported as absorbance at 517 nm normalized using absorbance at 700 nm.