Team:Freiburg/Attributions

Attributions

We want to thank everybody who helped us through every stage of our project. We did not only receive great advice or material for our project by diverse people but also strong support, which helped us to grow through every single challenge. Without these people, our project would not have been only half as successful. Thank you very much for this opportunity!

Student-Team

Tahira Aslan:

For the work on the In vitro, DAAO, inteins and Human Practice parts of the project.

Fabian Bäzner:

For the work on the intein expression system, BioBricks and Human Practice parts of the project.

Helena Engel:

For the work on the MIPD, in silico, functional assays and proteasome inhibition.

Felix Guischard:

For the work on chemical synthesis, in silico, Pymol and team-building activities.

Salomé Hahne:

For the work on MIPD, Human Practice and the intein expression system.

Paulina Kaas:

For the work in vivo, organising our collaborations, keeping our social media updated and especially on the beautiful figures for our wiki.

Fabian Krause:

For the work on in silico, software and for designing and implementing our wiki.

Janina Nandy:

For the work on chemical synthesis, in silico, functional assays, DAAO and collaborations.

Carolin Ruckes:

For the work on MIPD, the organization of tasks and reminding us on important deadlines.

Joshua Weygant:

For the work on sponsoring, human practice and entrepreneurship.

Alisa Zinina:

For the work on in vivo, racemase and for sponsoring efforts.

Principal Investigators

Prof. Barbara Di Ventura:

For her great input and keeping our focus on the wide range of our project, giving us supervision whenever we needed it and provided us with labspace and material.

Dr. Nicole Gensch:

For always being there for us, answering all our questions and for managing our finances.

Supervisors

Niklas Vesper:

For always reminding us how important negative controls are.

Philipp Franke:

For giving us advice on chemical synthesis.

Klara Lesch:

For helping us to find sponsors and solving our gel troubles.

Dennis Dombrovskij:

For troubleshooting our clonings.

Emir Bora Akmeric:

For the introduction of benchling for primer design and cloning, and for providing us with food, coffee and emotional support.

Enoch Boasiako Antwi:

For eternal weekend hours, for giving us a slot on the confocal, for the sharing his best primer design techniques and for the best theories and his great advice on our in vivo project.

Daniel Weis:

For always spreading optimism and useful knowledge as well as answering all our questions and sharing his big expertise in cloning.

Dr. Mehmet Ali Öztürk:

For giving us great advice on programs to use and the computational part of our project.

General Support

Dr. Tim Kunkel:

For managing the access to the buildings/rooms so that we were able to work 24/7.

Dr. Johannes Kaiser, University of Freiburg:

For managing the access to our lab and the BIOSS Signalhaus.

Dr. Jens Krüger from the BinAC in Tübingen:

For his advice on in silico and helping us with access to the BinAC computing cluster.

Dr. Günter Roth and Normann Kilb:

For measuring BLI with us and helping with the analysis of the results.

Project Advice

Dr. Hanna Wagner (Prof. Weber group, University of Freiburg):

For helping establishing our phage display, especially handling and amplifying the phages.

Dr. Steffen Wolf (Prof. Stock group, University of Freiburg):

For the realisation of the silico subproject, especially the handling of GROMACS and its forcefields - locally and on the BinAC.

Dr. Pavel Salavei (Core Facility Signalling Factory, University of Freiburg):

For giving us advice on the secretion of inteins, on expression and purification.

Dr. Anna Morath (Prof. Schamel group, University of Freiburg):

For giving us advice on T-Cell signaling FACS measurement protocols.

Dr. Susan Lauw (Core Facility Signalling Factory and Robotics Facility, University of Freiburg):

For helping us with the BLI measurement on the OctetRED and analyzing the results.

Vincent Idstein (Prof. Schamel group, University of Freiburg):

For providing phytochrome B to establish phage display and showing us how to do the calcium release measurement.

Nico Höfflin (Prof. Köhn group, University of Freiburg):

For advice on chemical synthesis and purification of L- and D-peptides.

Dominik Bezold (Prof. Jessen group, University of Freiburg):

For giving us guidance with the HPLC to analyse our self-synthesized peptides.

Navaneethan Palanisamy (Prof. Di Ventura group, University of Freiburg):

For giving us advice on optimizing diverse protocols.

Prof. Jan Münch and Ulm Peptide Therapeutics Centre:

For introducing us into the world of peptide therapeutics and organizing various talks to other experts.

Prof. Dieter Willbold, University of Düsseldorf:

For sharing his precious knowledge on D-Peptide therapeutics.

Dr. Frank Rosenau, University of Ulm:

For giving us an example of successful MIPD.

Dr. Joachim Boldt, University of Freiburg:

For discussing ethical aspects of synthetic biology and inspiring us to engage in public education.

Dr. Mary Williams:

For discussing with us how and why to engage the public about science.

Prof. Jeffrey Bode:

For advice on chemical synthesis and ligation methods.

Prof. Stephen Kent:

For advice on chemical synthesis and ligation methods.

Prof. Michael Kay:

For giving us advice on D-proteins and providing D-IZN.

Ahmed Mahmoud Abdellatef:

For his great support while writing the wiki.

Lab Equipment

Prof. Michael Kay, University of Utah:

For providing the D- and L-peptide IZN.

Dr. Stefan Schiller (esp. Lara Stühn), University of Freiburg:

For providing us with insights in the research of membraneless organelles as well as providing us with some of his constructs.

Prof. Gerald Radziwill, University of Freiburg:

For giving us access to his photospectrometer and also for providing us potential droplet forming proteins.

Edoardo Romano (Prof. Di Ventura group, University of Freiburg):

For providing us endless pBAD backbones, tryptone broth, induction protocols and advice.

Dr. Pierre Wehler (Prof. Di Ventura group, University of Freiburg):

For advice on lab equipments and for letting us use his protein purification buffers.

BinAC computing cluster Tübingen

Core Facility Signalling Factory and Robotics Facility, University of Freiburg (Shqipe Doroci, Dr. Pavel Salavei, Dr. Susan Lauw)

Prof. Köhn group, University of Freiburg

Prof. Römer group, University of Freiburg

Prof. Weber group, University of Freiburg

Prof. Schamel group, University of Freiburg