Utilizing bacterial cells for their capacity to serve as computationalrelating to or using computers or memory-like devices has potential applications in fields such as healthcare and biotechnology. Synthetic Cellular Recorders Integrating Biological Events (SCRIBE) uses a reverse transcriptasean enzyme that catalyzes the formation of DNA from an RNA template in reverse transcription enzyme to produce single stranded DNA which can be incorporated into the host-genome during DNA replication using the Beta Recombinasecatalyzes site-specific recombination protein which results in a mutation within the bacterial chromosome. This SCRIBE system can be applied to a large population of cells in order to measure the amount of a stimulus by sequencingdetermining the order of nucleotides in the DNA DNA to quantify the number of times the mutations occur in relation to the entire population. The UF iGEM team seeks to couple the SCRIBE system with the DNA nucleasean enzyme that cleaves the chains of nucleotides in nucleic acids into smaller units activity of the Cas9 protein to cut and kill the chromosome of the bacteria with wild type DNAtypical/normal form as it occurs in nature, thereby killing the cells without mutations.

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